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Article

Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro

  • Authors:
    • Mingming Xue
    • Yuru Zhou
    • Xiaoyu Liu
    • Dongsheng Ni
    • Yanxia Hu
    • Yaoshui Long
    • Pan Ju
    • Qin Zhou
  • View Affiliations / Copyright

    Affiliations: Division of Molecular Nephrology and The Creative Training Center for Undergraduates, M.O.E. Key Laboratory of Laboratory Medical Diagnostics, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China, The Seventh Class of 2012 Year Entry, Third Clinical College, Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 4315-4320
    |
    Published online on: September 22, 2016
       https://doi.org/10.3892/mmr.2016.5762
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Abstract

To seek out the potential microRNAs (miRNAs) that target Wilms' tumor suppressor 1 (WT1), a transcription factor required for progenitor proliferation as well as normal development of the kidney, and to clarify the effects of the miRNAs on WT1, the 3'-untranslated region (3'‑UTR) of WT1 was initially analyzed and miR‑743a, a seldom‑reported miRNA, was identified. In the present paper, luciferase reporter assays were performed to confirm that miR‑743a is able to directly target the 3'‑UTR of WT1. Subsequently, reverse transcription‑quantitative polymerase chain reaction, combined with western blotting analyses, were performed, and the results revealed a significant inhibition of WT1 at the mRNA and the protein levels. Furthermore, a 5‑ethynyl‑2'‑deoxyuridine (EdU) cell proliferation assay, coupled with a WT1 rescue strategy, demonstrated that miR‑743a inhibited the proliferation of metanephric mesenchymal (MM) cells, in part by targeting WT1. In conclusion, by targeting WT1, miR‑743a suppresses the proliferation of MM cells in vitro, and probably possesses vital functions in kidney development and kidney‑associated diseases.
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1 

Dressler GR: The cellular basis of kidney development. Annu Rev Cell Dev Biol. 22:509–529. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Little MH and McMahon AP: Mammalian kidney development: Principles, progress, and projections. Cold Spring Harb Perspect Biol. 4(pii): a0083002012.PubMed/NCBI

3 

Lindström NO, Lawrence ML, Burn SF, Johansson JA, Bakker ER, Ridgway RA, Chang CH, Karolak MJ, Oxburgh L, Headon DJ, et al: Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron. Elife. 3:e040002015.PubMed/NCBI

4 

Motamedi FJ, Badro DA, Clarkson M, Lecca MR, Bradford ST, Buske FA, Saar K, Hübner N, Brändli AW and Schedl A: WT1 controls antagonistic FGF and BMP-pSMAD pathways in early renal progenitors. Nat Commun. 5:44442014. View Article : Google Scholar : PubMed/NCBI

5 

Armstrong JF, Pritchard-Jones K, Bickmore WA, Hastie ND and Bard JB: The expression of the Wilms' tumour gene, WT1, in the developing mammalian embryo. Mech Dev. 40:85–97. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Pritchard-Jones K, Fleming S, Davidson D, Bickmore W, Porteous D, Gosden C, Bard J, Buckler A, Pelletier J, Housman D, et al: The candidate Wilms' tumour gene is involved in genitourinary development. Nature. 346:194–197. 1990. View Article : Google Scholar : PubMed/NCBI

7 

Berry RL, Ozdemir DD, Aronow B, Lindström NO, Dudnakova T, Thornburn A, Perry P, Baldock R, Armit C, Joshi A, et al: Deducing the stage of origin of Wilms' tumours from a developmental series of Wt1-mutant mice. Dis Model Mech. 8:903–917. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Kirschner KM, Braun JF, Jacobi CL, Rudigier LJ, Persson AB and Scholz H: Amine oxidase copper-containing 1 (AOC1) is a downstream target gene of the Wilms tumor protein, WT1, during kidney development. J Biol Chem. 289:24452–24462. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Donovan MJ, Natoli TA, Sainio K, Amstutz A, Jaenisch R, Sariola H and Kreidberg JA: Initial differentiation of the metanephric mesenchyme is independent of WT1 and the ureteric bud. Dev Genet. 24:252–262. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Cannell IG, Kong YW and Bushell M: How do microRNAs regulate gene expression? Biochem Soc Trans. 36:1224–1231. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Wang Z: miRNA in the regulation of ion channel/transporter expression. Compr Physiol. 3:599–653. 2013.PubMed/NCBI

12 

Alvarez-Garcia I and Miska EA: MicroRNA functions in animal development and human disease. Development. 132:4653–4662. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Ho J and Kreidberg JA: The long and short of microRNAs in the kidney. J Am Soc Nephrol. 23:400–404. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Lyu Z, Mao Z, Wang H, Fang Y, Chen T, Wan Q, Wang M, Wang N, Xiao J, Wei H, et al: MiR-181b targets Six2 and inhibits the proliferation of metanephric mesenchymal cells in vitro. Biochem Biophys Res Commun. 440:495–501. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Lv X, Mao Z, Lyu Z, Zhang P, Zhan A, Wang J, Yang H, Li M, Wang H, Wan Q, et al: miR181c promotes apoptosis and suppresses proliferation of metanephric mesenchyme cells by targeting Six2 in vitro. Cell Biochem Funct. 32:571–579. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Yang X, Wang X, Nie F, Liu T, Yu X, Wang H, Li Q, Peng R, Mao Z, Zhou Q and Li G: miR-135 family members mediate podocyte injury through the activation of Wnt/β-catenin signaling. Int J Mol Med. 36:669–677. 2015.PubMed/NCBI

17 

Sun H, Li QW, Lv XY, Ai JZ, Yang QT, Duan JJ, Bian GH, Xiao Y, Wang YD, Zhang Z, et al: MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation. Mol Biol Rep. 37:2951–2958. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Ranganathan P, Jayakumar C, Tang Y, Park KM, Teoh JP, Su H, Li J, Kim IM and Ramesh G: MicroRNA-150 deletion in mice protects kidney from myocardial infarction-induced acute kidney injury. Am J Physiol Renal Physiol. 309:F551–F558. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Hastie ND: The genetics of Wilms' tumor - a case of disrupted development. Annu Rev Genet. 28:523–558. 1994. View Article : Google Scholar : PubMed/NCBI

21 

Dong L, Pietsch S and Englert C: Towards an understanding of kidney diseases associated with WT1 mutations. Kidney Int. 88:684–690. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Eckardt KU, Coresh J, Devuyst O, Johnson RJ, Köttgen A, Levey AS and Levin A: Evolving importance of kidney disease: From subspecialty to global health burden. Lancet. 382:158–169. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xue M, Zhou Y, Liu X, Ni D, Hu Y, Long Y, Ju P and Zhou Q: Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro. Mol Med Rep 14: 4315-4320, 2016.
APA
Xue, M., Zhou, Y., Liu, X., Ni, D., Hu, Y., Long, Y. ... Zhou, Q. (2016). Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro. Molecular Medicine Reports, 14, 4315-4320. https://doi.org/10.3892/mmr.2016.5762
MLA
Xue, M., Zhou, Y., Liu, X., Ni, D., Hu, Y., Long, Y., Ju, P., Zhou, Q."Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro". Molecular Medicine Reports 14.5 (2016): 4315-4320.
Chicago
Xue, M., Zhou, Y., Liu, X., Ni, D., Hu, Y., Long, Y., Ju, P., Zhou, Q."Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro". Molecular Medicine Reports 14, no. 5 (2016): 4315-4320. https://doi.org/10.3892/mmr.2016.5762
Copy and paste a formatted citation
x
Spandidos Publications style
Xue M, Zhou Y, Liu X, Ni D, Hu Y, Long Y, Ju P and Zhou Q: Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro. Mol Med Rep 14: 4315-4320, 2016.
APA
Xue, M., Zhou, Y., Liu, X., Ni, D., Hu, Y., Long, Y. ... Zhou, Q. (2016). Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro. Molecular Medicine Reports, 14, 4315-4320. https://doi.org/10.3892/mmr.2016.5762
MLA
Xue, M., Zhou, Y., Liu, X., Ni, D., Hu, Y., Long, Y., Ju, P., Zhou, Q."Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro". Molecular Medicine Reports 14.5 (2016): 4315-4320.
Chicago
Xue, M., Zhou, Y., Liu, X., Ni, D., Hu, Y., Long, Y., Ju, P., Zhou, Q."Proliferation of metanephric mesenchymal cells is inhibited by miR-743a-mediated WT1 suppression in vitro". Molecular Medicine Reports 14, no. 5 (2016): 4315-4320. https://doi.org/10.3892/mmr.2016.5762
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