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Article

Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells

  • Authors:
    • Ye Chan Sim
    • Jong Seok Lee
    • Sarah Lee
    • Youn Kyoung Son
    • Jung‑Eun Park
    • Jeong Eun Song
    • Suk‑Jin Ha
    • Eock Kee Hong
  • View Affiliations / Copyright

    Affiliations: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, National Institute of Biological Resources, Incheon 22689, Republic of Korea
  • Pages: 4263-4270
    |
    Published online on: September 22, 2016
       https://doi.org/10.3892/mmr.2016.5763
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Abstract

The purpose of the present study was to elucidate the cytoprotective effects of polysaccharides isolated from Inonotus obliquus. The polysaccharides were extracted from the fruiting body of I. obliquus (PFIO) and the liquid culture broth of I. obliquus (PLIO). The effects of PFIO and PLIO on hydrogen peroxide (H2O2)‑induced oxidative damage of RINm5F pancreatic β‑cells were comparatively investigated using an MTT assay, immunofluorescent staining, flow cytometry, and western blot analyses in vitro. The results of the present study demonstrated that treatment with PFIO and PLIO decreased DNA fragmentation and the rate of apoptosis. In addition, pretreatment of cells with PFIO and PLIO prior to H2O2 exposure resulted in increased insulin secretion and scavenging activity for intracellular reactive oxygen species, as compared with treatment with H2O2 alone. The results of the present study suggested that PFIO and PLIO may exert protective effects against H2O2‑induced oxidative stress via the regulation of mitogen‑activated protein kinases, nuclear factor‑κB and apoptotic proteins. Therefore, PFIO and PLIO may have potential merit as a medicinal food for the prevention of diabetes.
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Copy and paste a formatted citation
Spandidos Publications style
Sim YC, Lee JS, Lee S, Son YK, Park JE, Song JE, Ha SJ and Hong EK: Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells. Mol Med Rep 14: 4263-4270, 2016.
APA
Sim, Y.C., Lee, J.S., Lee, S., Son, Y.K., Park, J., Song, J.E. ... Hong, E.K. (2016). Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells. Molecular Medicine Reports, 14, 4263-4270. https://doi.org/10.3892/mmr.2016.5763
MLA
Sim, Y. C., Lee, J. S., Lee, S., Son, Y. K., Park, J., Song, J. E., Ha, S., Hong, E. K."Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells". Molecular Medicine Reports 14.5 (2016): 4263-4270.
Chicago
Sim, Y. C., Lee, J. S., Lee, S., Son, Y. K., Park, J., Song, J. E., Ha, S., Hong, E. K."Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells". Molecular Medicine Reports 14, no. 5 (2016): 4263-4270. https://doi.org/10.3892/mmr.2016.5763
Copy and paste a formatted citation
x
Spandidos Publications style
Sim YC, Lee JS, Lee S, Son YK, Park JE, Song JE, Ha SJ and Hong EK: Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells. Mol Med Rep 14: 4263-4270, 2016.
APA
Sim, Y.C., Lee, J.S., Lee, S., Son, Y.K., Park, J., Song, J.E. ... Hong, E.K. (2016). Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells. Molecular Medicine Reports, 14, 4263-4270. https://doi.org/10.3892/mmr.2016.5763
MLA
Sim, Y. C., Lee, J. S., Lee, S., Son, Y. K., Park, J., Song, J. E., Ha, S., Hong, E. K."Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells". Molecular Medicine Reports 14.5 (2016): 4263-4270.
Chicago
Sim, Y. C., Lee, J. S., Lee, S., Son, Y. K., Park, J., Song, J. E., Ha, S., Hong, E. K."Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells". Molecular Medicine Reports 14, no. 5 (2016): 4263-4270. https://doi.org/10.3892/mmr.2016.5763
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