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Article

Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK

  • Authors:
    • Juan Zhou
    • Yinghua Li
    • Lina Yang
    • Yougen Wu
    • Yunjiao Zhou
    • Yunqing Cui
    • Gong Yang
    • Yang Hong
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China
  • Pages: 5653-5659
    |
    Published online on: November 16, 2016
       https://doi.org/10.3892/mmr.2016.5951
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Abstract

Mammalian stanniocalcin 2 (STC2) is a glycoprotein hormone with multiple functions. The present study determined the importance of STC2 in osteoblast differentiation. It was revealed that the expression of STC2 was increased during the differentiation of MC3T3-E1 cells to osteoblasts and that knockdown of STC2 reduced osteoblast differentiation and mineralization, whereas STC2 overexpression increased differentiation and mineralization. Knockdown of STC2 downregulated the expression of osteoblast-associated genes, including runt‑related transcription factor 2, collagen type I α 1 chain, osterix and osteocalcin. Overexpression of STC2 upregulated the expression of these osteoblastic genes. In addition, overexpression of STC2 enhanced the phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK1/2), whereas inhibition of ERK phosphorylation reduced osteoblast differentiation of MC3T3‑E1 cells overexpressing STC2. These findings indicated that STC2 may promote osteoblast differentiation and mineralization by regulating ERK activation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Li Y, Yang L, Wu Y, Zhou Y, Cui Y, Yang G and Hong Y: Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK. Mol Med Rep 14: 5653-5659, 2016.
APA
Zhou, J., Li, Y., Yang, L., Wu, Y., Zhou, Y., Cui, Y. ... Hong, Y. (2016). Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK. Molecular Medicine Reports, 14, 5653-5659. https://doi.org/10.3892/mmr.2016.5951
MLA
Zhou, J., Li, Y., Yang, L., Wu, Y., Zhou, Y., Cui, Y., Yang, G., Hong, Y."Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK". Molecular Medicine Reports 14.6 (2016): 5653-5659.
Chicago
Zhou, J., Li, Y., Yang, L., Wu, Y., Zhou, Y., Cui, Y., Yang, G., Hong, Y."Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK". Molecular Medicine Reports 14, no. 6 (2016): 5653-5659. https://doi.org/10.3892/mmr.2016.5951
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Li Y, Yang L, Wu Y, Zhou Y, Cui Y, Yang G and Hong Y: Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK. Mol Med Rep 14: 5653-5659, 2016.
APA
Zhou, J., Li, Y., Yang, L., Wu, Y., Zhou, Y., Cui, Y. ... Hong, Y. (2016). Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK. Molecular Medicine Reports, 14, 5653-5659. https://doi.org/10.3892/mmr.2016.5951
MLA
Zhou, J., Li, Y., Yang, L., Wu, Y., Zhou, Y., Cui, Y., Yang, G., Hong, Y."Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK". Molecular Medicine Reports 14.6 (2016): 5653-5659.
Chicago
Zhou, J., Li, Y., Yang, L., Wu, Y., Zhou, Y., Cui, Y., Yang, G., Hong, Y."Stanniocalcin 2 improved osteoblast differentiation via phosphorylation of ERK". Molecular Medicine Reports 14, no. 6 (2016): 5653-5659. https://doi.org/10.3892/mmr.2016.5951
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