Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation

  • Authors:
    • Mingxing Zheng
    • Zhibing Zhu
    • Yongzhao Zhao
    • Da Yao
    • Maoqing Wu
    • Gengyun Sun
  • View Affiliations

  • Published online on: December 8, 2016     https://doi.org/10.3892/mmr.2016.6008
  • Pages: 375-379
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Abstract

Previous studies have demonstrated that oridonin, a tetracyclic diterpenoid compound extracted from Rabdosia rubescens, inhibits proliferation and induces apoptosis in several tumor cell lines. However, the mechanism by which oridonin inhibits the cell cycle remains poorly understood. In the present study, possible mechanisms by which oridonin affects cell cycle progression were explored in A549 lung cancer cells. Flow cytometry analysis indicated that oridonin inhibited the proliferation of A549 cells by inducing G2/M cell cycle arrest in a dose‑dependent manner. Western blot analysis revealed that in oridonin treated cells, phosphorylated (p‑)ATM serine/threonine kinase (S1981), p‑checkpoint kinase 2 (CHK2) (T68), p‑p53, and phosphorylated H2A histone family member X protein levels were visibly increased, indicating that oridonin promoted G2/M arrest in A549 cells through the ATM‑p53‑CHK2 pathway. This data suggests that oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation, which is likely a common mechanism in other tumor cell types when using this drug for cancer treatment.
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January-2017
Volume 15 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zheng M, Zhu Z, Zhao Y, Yao D, Wu M and Sun G: Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation. Mol Med Rep 15: 375-379, 2017
APA
Zheng, M., Zhu, Z., Zhao, Y., Yao, D., Wu, M., & Sun, G. (2017). Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation. Molecular Medicine Reports, 15, 375-379. https://doi.org/10.3892/mmr.2016.6008
MLA
Zheng, M., Zhu, Z., Zhao, Y., Yao, D., Wu, M., Sun, G."Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation". Molecular Medicine Reports 15.1 (2017): 375-379.
Chicago
Zheng, M., Zhu, Z., Zhao, Y., Yao, D., Wu, M., Sun, G."Oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation". Molecular Medicine Reports 15, no. 1 (2017): 375-379. https://doi.org/10.3892/mmr.2016.6008