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Article Open Access

ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer

  • Authors:
    • Shuixiang Tao
    • Shuai Meng
    • Xiangyi Zheng
    • Liping Xie
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1143-1148
    |
    Published online on: January 24, 2017
       https://doi.org/10.3892/mmr.2017.6141
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Abstract

Ellipticine, an alkaloid isolated from Apocyanaceae plants, has been demonstrated to exhibit antitumor activity in several cancers. However, the effect and the mechanisms underlying its action have not been investigated in human bladder cancer cells. The aim of the present study was to investigate the effect and mechanism of ellipticine on the behavior of T‑24 bladder cancer cells. T‑24 cells were treated with varying concentrations and durations of ellipticine. Cell viability was evaluated by Cell Counting Kit‑8 assay. Cell motility was analyzed by Transwell migration assay. Flow cytometry, reverse transcription‑quantitative polymerase chain reaction and western blot analyses were performed to detect the cell cycle and signaling pathways involved. The results demonstrated that ellipticine suppressed proliferation and inhibited the migration ability of T‑24 bladder cancer cells in a dose‑ and time‑dependent manner, and resulted in G2/M cell cycle arrest. The mechanism of this action was demonstrated to be due to ellipticine‑triggered activation of the ATM serine/threonine kinase pathway. These data therefore suggest that ellipticine may be effective towards treating human bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tao S, Meng S, Zheng X and Xie L: ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer. Mol Med Rep 15: 1143-1148, 2017.
APA
Tao, S., Meng, S., Zheng, X., & Xie, L. (2017). ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer. Molecular Medicine Reports, 15, 1143-1148. https://doi.org/10.3892/mmr.2017.6141
MLA
Tao, S., Meng, S., Zheng, X., Xie, L."ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer". Molecular Medicine Reports 15.3 (2017): 1143-1148.
Chicago
Tao, S., Meng, S., Zheng, X., Xie, L."ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer". Molecular Medicine Reports 15, no. 3 (2017): 1143-1148. https://doi.org/10.3892/mmr.2017.6141
Copy and paste a formatted citation
x
Spandidos Publications style
Tao S, Meng S, Zheng X and Xie L: ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer. Mol Med Rep 15: 1143-1148, 2017.
APA
Tao, S., Meng, S., Zheng, X., & Xie, L. (2017). ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer. Molecular Medicine Reports, 15, 1143-1148. https://doi.org/10.3892/mmr.2017.6141
MLA
Tao, S., Meng, S., Zheng, X., Xie, L."ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer". Molecular Medicine Reports 15.3 (2017): 1143-1148.
Chicago
Tao, S., Meng, S., Zheng, X., Xie, L."ATM participates in the regulation of viability and cell cycle via ellipticine in bladder cancer". Molecular Medicine Reports 15, no. 3 (2017): 1143-1148. https://doi.org/10.3892/mmr.2017.6141
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