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Article

Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells

  • Authors:
    • Zhou Song
    • Bo Wei
    • Canrong Lu
    • Xiaohui Huang
    • Peiyu Li
    • Lin Chen
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, General Hospital of Chinese PLA, Beijing 100853, P.R. China
  • Pages: 1909-1915
    |
    Published online on: February 15, 2017
       https://doi.org/10.3892/mmr.2017.6205
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Abstract

The traditional anti-diabetic drug, metformin, has been found to have anticancer effects. The Sonic hedgehog (Shh) signaling pathway is involved in the on cogenesis of gastric cancer. The aim of the present study was to investigate whether metformin has an effect on the Shh signaling pathway in gastric cancer cells. HGC‑27 and MKN‑45 human gastric cancer cells were treated with metformin at different concentrations and for different durations. Subsequently the mRNA and protein levels of Shh, Smoothened (SMO), and Glioma‑associated oncogene (Gli)‑1, Gli‑2 and Gli‑3 were examined using western blot and reverse transcription‑quantitative polymerase chain reaction analyses. RNA interference was used to detect whether the effects of metformin treatment on the Shh signaling pathway were dependent on AMP‑activated protein kinase (AMPK). The results revealed that the protein and mRNA levels of Shh and Gli‑1 were decreased by metformin treatment in the two cell lines in a dose‑ and time‑dependent manner. Metformin also significantly inhibited the gene and protein expression levels of SMO, Gli‑2 and Gli‑3. The small interfering RNA‑induced depletion of AMPK reversed the suppressive effect of metformin on recombinant human Shh‑induced expression of Gli‑1 in HGC‑27 gastric cancer cells. Therefore, metformin inhibited the Shh signaling pathway in the gastric cancer cell lines and the inhibitory effect of metformin on the Shh pathway was AMPK-dependent.
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Copy and paste a formatted citation
Spandidos Publications style
Song Z, Wei B, Lu C, Huang X, Li P and Chen L: Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells. Mol Med Rep 15: 1909-1915, 2017.
APA
Song, Z., Wei, B., Lu, C., Huang, X., Li, P., & Chen, L. (2017). Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells. Molecular Medicine Reports, 15, 1909-1915. https://doi.org/10.3892/mmr.2017.6205
MLA
Song, Z., Wei, B., Lu, C., Huang, X., Li, P., Chen, L."Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells". Molecular Medicine Reports 15.4 (2017): 1909-1915.
Chicago
Song, Z., Wei, B., Lu, C., Huang, X., Li, P., Chen, L."Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells". Molecular Medicine Reports 15, no. 4 (2017): 1909-1915. https://doi.org/10.3892/mmr.2017.6205
Copy and paste a formatted citation
x
Spandidos Publications style
Song Z, Wei B, Lu C, Huang X, Li P and Chen L: Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells. Mol Med Rep 15: 1909-1915, 2017.
APA
Song, Z., Wei, B., Lu, C., Huang, X., Li, P., & Chen, L. (2017). Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells. Molecular Medicine Reports, 15, 1909-1915. https://doi.org/10.3892/mmr.2017.6205
MLA
Song, Z., Wei, B., Lu, C., Huang, X., Li, P., Chen, L."Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells". Molecular Medicine Reports 15.4 (2017): 1909-1915.
Chicago
Song, Z., Wei, B., Lu, C., Huang, X., Li, P., Chen, L."Metformin suppresses the expression of Sonic hedgehog in gastric cancer cells". Molecular Medicine Reports 15, no. 4 (2017): 1909-1915. https://doi.org/10.3892/mmr.2017.6205
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