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Article

Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells

  • Authors:
    • Jing Zhao
    • Mei Zhang
    • Pengcheng He
    • Junjie Zhao
    • Ying Chen
    • Jun Qi
    • Yuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Institute of Xi'an Blood Bank, Shaanxi Blood Center, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 1807-1815
    |
    Published online on: February 17, 2017
       https://doi.org/10.3892/mmr.2017.6213
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Abstract

Oridonin is a diterpenoid compound isolated from the medicinal herb Rabdosia rubescens, and has shown marked antitumor effects against different types of cancer. However, the definitive systematic molecular mechanism underlying the antitumor activity of oridonin in multiple myeloma remains to be elucidated. In the present study, cell viability and cytotoxicity were examined to determine the appropriate concentration for proteomic investigation. In addition, cell apoptosis was evaluated using flow cytometry and transmission electron microscopy. A proteomic investigation using a two‑dimensional electrophoresis system and mass spectrometry was performed to identify and characterize the global proteome of the apoptosis induced by oridonin. Of the proteins identified, seven were involved in the anticancer effects of oridonin. Regulation of the expression and function of target proteins, stathmin, dihydrofolate reductase and pyruvate dehydrogenase E1β, may be potential, therapeutic strategies to effectively treat multiple myeloma. These findings provide novel information on the molecular mechanisms underlying the anticancer properties of oridonin in multiple myeloma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Zhang M, He P, Zhao J, Chen Y, Qi J and Wang Y: Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells. Mol Med Rep 15: 1807-1815, 2017.
APA
Zhao, J., Zhang, M., He, P., Zhao, J., Chen, Y., Qi, J., & Wang, Y. (2017). Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells. Molecular Medicine Reports, 15, 1807-1815. https://doi.org/10.3892/mmr.2017.6213
MLA
Zhao, J., Zhang, M., He, P., Zhao, J., Chen, Y., Qi, J., Wang, Y."Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells". Molecular Medicine Reports 15.4 (2017): 1807-1815.
Chicago
Zhao, J., Zhang, M., He, P., Zhao, J., Chen, Y., Qi, J., Wang, Y."Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells". Molecular Medicine Reports 15, no. 4 (2017): 1807-1815. https://doi.org/10.3892/mmr.2017.6213
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Zhang M, He P, Zhao J, Chen Y, Qi J and Wang Y: Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells. Mol Med Rep 15: 1807-1815, 2017.
APA
Zhao, J., Zhang, M., He, P., Zhao, J., Chen, Y., Qi, J., & Wang, Y. (2017). Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells. Molecular Medicine Reports, 15, 1807-1815. https://doi.org/10.3892/mmr.2017.6213
MLA
Zhao, J., Zhang, M., He, P., Zhao, J., Chen, Y., Qi, J., Wang, Y."Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells". Molecular Medicine Reports 15.4 (2017): 1807-1815.
Chicago
Zhao, J., Zhang, M., He, P., Zhao, J., Chen, Y., Qi, J., Wang, Y."Proteomic analysis of oridonin-induced apoptosis in multiple myeloma cells". Molecular Medicine Reports 15, no. 4 (2017): 1807-1815. https://doi.org/10.3892/mmr.2017.6213
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