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Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis

  • Authors:
    • Lorena Coretti
    • Mariella Cuomo
    • Ermanno Florio
    • Domenico Palumbo
    • Simona Keller
    • Raffaela Pero
    • Lorenzo Chiariotti
    • Francesca Lembo
    • Carlo Cafiero
  • View Affiliations / Copyright

    Affiliations: Institute of Experimental Endocrinology and Oncology, National Research Council, I‑80131 Naples, Italy, Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, I‑80131 Naples, Italy, Department of Pharmacy, University of Naples Federico II, I‑80131 Naples, Italy, Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, I‑80131 Naples, Italy
    Copyright: © Coretti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2007-2014
    |
    Published online on: March 1, 2017
       https://doi.org/10.3892/mmr.2017.6269
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Abstract

Periodontitis is one of the most common oral inflammatory diseases, and results in connective tissue degradation and gradual tooth loss. It manifests with formation of periodontal pockets, in which anaerobic and Gram‑negative bacteria proliferate rapidly. Consequently, alteration of the subgingival microbiota is considered the primary etiologic agent of periodontitis. Previous studies have reported that smokers are at increased risk of periodontal disease, in both prevalence and severity, indicating that smoking is a risk factor for the onset and progression of the pathology. In the present study, 16S rRNA sequencing was employed to assess the subgingival microbiota in 6 smoker patients with chronic periodontitis, 6 non‑smoker patients with chronic periodontitis and 8 healthy controls. The results demonstrated significant alterations in the microbial structure of periodontitis patients. High relative abundance of Parvimonans, Desulfubulbus, Paludibacter, Haemophilus, and Sphaerochaeta genera characterized subgingival microbiota of periodontitis patients, both smokers and non‑smokers. Due to the high precision and sensitivity of the 16S rRNA sequencing method, analysis for low‑abundant genera (including Pedobacter, Granulicatella, Paracoccus, Atopobium, Bifidobacterium, Coprococcus, Oridobacteriu, Peptococcus, Oscillospira and Akkermansia) was feasible, and revealed novel phylotypes associated with periodontitis. Of note, a major microbial community alteration was evident in smoker patients, suggesting an association between smoking and severity of subgingival dysbiosis. The present study confirmed that chronic periodontitis is a polymicrobial disease where changes in the equilibrium of subgingival microbiota contribute to severity of pathology.
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1 

Avila M, Ojcius DM and Yilmaz O: The oral microbiota: Living with a permanent guest. DNA Cell Biol. 28:405–411. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Cho I and Blaser MJ: The human microbiome: At the interface of health and disease. Nat Rev Genet. 13:260–270. 2012.PubMed/NCBI

3 

Haffajee AD, Teles RP and Socransky SS: The effect of periodontal therapy on the composition of the subgingival microbiota. Periodontol 2000. 42:219–258. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Dentino A, Lee S, Mailhot J and Hefti AF: Principles of periodontology. Periodontol 2000. 61:16–53. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Al Harthi LS, Cullinan MP, Leichter JW and Thomson WM: The impact of periodontitis on oral health-related quality of life: A review of the evidence from observational studies. Aust Dent J. 58:274–277; quiz 384. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Seymour GJ, Ford PJ, Cullinan MP, Leishman S and Yamazaki K: Relationship between periodontal infections and systemic disease. Clin Microbiol Infect. 13 Suppl 4:S3–S10. 2007. View Article : Google Scholar

7 

Armitage CG: Comparison of the microbiological features of chronic and aggressive periodontitis. Periodontol 2000. 53:70–88. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Socransky SS, Haffajee AD, Cugini MA, Smith C and Kent RL Jr: Microbial complexes in subgingival plaque. J Clin Periodontol. 25:134–44. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Page RC and Kornman KS: The pathogenesis of human periodontitis: An introduction. Periodontol 2000. 14:9–11. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Lee J, Taneja V and Vassallo R: Cigarette smoking and inflammation: Cellular and molecular mechanisms. J Dent Res. 91:142–149. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Labriola A, Needleman I and Moles DR: Systematic review of the effect of smoking on nonsurgical periodontal therapy. Periodontol 2000. 37:124–137. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Haffajee AD and Socransky SS: Relationship of cigarette smoking to the subgingival microbiota. J Clin Periodontol. 28:377–88. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Preber H, Bergström J and Linder LE: Occurrence of periopathogens in smoker and non-smoker patients. J Clin Periodontol. 19:667–671. 1992. View Article : Google Scholar : PubMed/NCBI

14 

Van der Velden U, Varoufaki A, Hutter JW, Xu L, Timmerman MF, Van Winkelhoff AJ and Loos BG: Effect of smoking and periodontal treatment on the subgingival microflora. J Clin Periodontol. 30:603–610. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner AC, Yu WH, Lakshmanan A and Wade WG: The human oral microbiome. J Bacteriol. 192:5002–5017. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Sammartino G, Tia M, Gentile E, Marenzi G and Claudio PP: Platelet-rich plasma and resorbable membrane for prevention of periodontal defects after deeply impacted lower third molar extraction. J Oral Maxillofac Surg. 67:2369–2373. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Ramfjord SP: Present status of the modified Widman flap procedure. J Periodontol. 48:558–565. 1977. View Article : Google Scholar : PubMed/NCBI

18 

Illumina. 16S Metagenomic Sequencing Library Preparation. 2013.http://support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html

19 

Mizrahi-Man O, Davenport ER and Gilad Y: Taxonomic classification of bacterial 16S rRNA genes using short sequencing reads: Evaluation of effective study designs. PLoS One. 8:e536082013. View Article : Google Scholar : PubMed/NCBI

20 

Illumina. MiSeq System Denature and Dilute Libraries Guide. 2016.http://support.illumina.com/downloads/prepare_libraries_for_sequencing_miseq_15039740.html

21 

Zhang J, Kobert K, Flouri T and Stamatakis A: PEAR: A fast and accurate Illumina Paired-End reAd mergeR. Bioinformatics. 30:614–620. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Schmieder R and Edwards R: Quality control and preprocessing of metagenomic datasets. Bioinformatics. 27:863–864. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, et al: QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 7:335–336. 2010. View Article : Google Scholar : PubMed/NCBI

24 

DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P and Andersen GL: Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol. 72:5069–5072. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Clarke KR: Non-parametric multivariate analyses of changes in community structure. Aust J Ecol. 18:117–143. 1993. View Article : Google Scholar

26 

Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS and Huttenhower C: Metagenomic biomarker discovery and explanation. Genome Biol. 12:R602011. View Article : Google Scholar : PubMed/NCBI

27 

Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes J, Clemente JC, Burkepile DE, Thurber RL Vega, Knight R, et al: Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 31:814–821. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Kanehisa M and Goto S: KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28:27–30. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Kim M, Morrison M and Yu Z: Evaluation of different partial 16S rRNA gene sequence regions for phylogenetic analysis of microbiomes. J Microbiol Methods. 84:81–87. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Hug LA, Baker BJ, Anantharaman K, Brown CT, Probst AJ, Castelle CJ, Butterfield CN, Hernsdorf AW, Amano Y, Ise K, et al: A new view of the tree of life. Nat Microbiol. 1:160482016. View Article : Google Scholar : PubMed/NCBI

31 

Wang J, Qi J, Zhao H, He S, Zhang Y, Wei S and Zhao F: Metagenomic sequencing reveals microbiota and its functional potential associated with periodontal disease. Sci Rep. 3:18432013.PubMed/NCBI

32 

Kirst ME, Li EC, Alfant B, Chi YY, Walker C, Magnusson I and Wang GP: Dysbiosis and alterations in predicted functions of the subgingival microbiome in chronic periodontitis. Appl Environ Microbiol. 81:783–793. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Arora N, Mishra A and Chugh S: Microbial role in periodontitis: Have we reached the top? Some unsung bacteria other than red complex. J Indian Soc Periodontol. 18:9–13. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Bizzarro S, Loos BG, Laine ML, Crielaard W and Zaura E: Subgingival microbiome in smokers and non-smokers in periodontitis: An exploratory study using traditional targeted techniques and a next-generation sequencing. J Clin Periodontol. 40:483–492. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, Socransky SS, Hasturk H, Van Dyke TE, Dewhirst F and Paster BJ: Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol. 80:1421–1432. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Gupta RS, Mahmood S and Adeolu M: Erratum: A phylogenomic and molecular signature based approach for characterization of the Phylum Spirochaetes and its major clades: Proposal for a taxonomic revision of the phylum. Front Microbiol. 4:3222013. View Article : Google Scholar : PubMed/NCBI

37 

Bergeron MG, Simard P and Provencher P: Influence of growth medium and supplement on growth of Haemophilus influenzae and on antibacterial activity of several antibiotics. J Clin Microbiol. 25:650–655. 1987.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Coretti L, Cuomo M, Florio E, Palumbo D, Keller S, Pero R, Chiariotti L, Lembo F and Cafiero C: Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis. Mol Med Rep 15: 2007-2014, 2017.
APA
Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R. ... Cafiero, C. (2017). Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis. Molecular Medicine Reports, 15, 2007-2014. https://doi.org/10.3892/mmr.2017.6269
MLA
Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R., Chiariotti, L., Lembo, F., Cafiero, C."Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis". Molecular Medicine Reports 15.4 (2017): 2007-2014.
Chicago
Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R., Chiariotti, L., Lembo, F., Cafiero, C."Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis". Molecular Medicine Reports 15, no. 4 (2017): 2007-2014. https://doi.org/10.3892/mmr.2017.6269
Copy and paste a formatted citation
x
Spandidos Publications style
Coretti L, Cuomo M, Florio E, Palumbo D, Keller S, Pero R, Chiariotti L, Lembo F and Cafiero C: Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis. Mol Med Rep 15: 2007-2014, 2017.
APA
Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R. ... Cafiero, C. (2017). Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis. Molecular Medicine Reports, 15, 2007-2014. https://doi.org/10.3892/mmr.2017.6269
MLA
Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R., Chiariotti, L., Lembo, F., Cafiero, C."Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis". Molecular Medicine Reports 15.4 (2017): 2007-2014.
Chicago
Coretti, L., Cuomo, M., Florio, E., Palumbo, D., Keller, S., Pero, R., Chiariotti, L., Lembo, F., Cafiero, C."Subgingival dysbiosis in smoker and non‑smoker patients with chronic periodontitis". Molecular Medicine Reports 15, no. 4 (2017): 2007-2014. https://doi.org/10.3892/mmr.2017.6269
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