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Article Open Access

Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress

  • Authors:
    • Yujie Liu
    • Xizhou Chen
    • Jie Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Tongchuan Kuangwuju Central Hospital, Shaanxi Coal and Chemical Industry Group, Tongchuan, Shaanxi 727000, P.R. China, Department of General Medicine, Baoji Central Hospital, Baoji, Shaanxi 721008, P.R. China, Department of Anesthesiology, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2457-2464
    |
    Published online on: March 9, 2017
       https://doi.org/10.3892/mmr.2017.6304
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Abstract

Resveratrol, a natural phytochemical found in grapes and red wine, has been found to possess protective effects against endothelial cell apoptosis and oxidative damage. Oxidized‑low density lipoprotein (ox‑LDL) can induce apoptosis of endothelial cells, which is an important initial event in several cardiovascular diseases. However, the effect of resveratrol on ox‑LDL‑induced apoptosis and oxidative damage, and the possible associated mechanisms remain to be elucidated. In the present study, following exposure to ox‑LDL, human umbilical vein endothelial cells (HUVECs) were treated with or without resveratrol. Cell viability was examined using Cell Counting Kit‑8 and 5‑bromo‑2'‑deoxyuridine uptake assays, respectively. Cell apoptosis was determined by flow cytometry. Apoptosis‑associated markers were detected using western blot analysis. Oxidative stress was analyzed using molecular and biochemical approaches. Resveratrol restored ox‑LDL‑induced HUVEC injury and apoptosis in a dose‑dependent manner. In addition, compared with the control group, ox‑LDL treatment decreased the B cell lymphoma‑2 (Bcl‑2)/Bcl‑2‑associated X protein ratio, mitochondrial membrane potential and activation of superoxide dismutase, and enhanced the release of mitochondrial cytochrome c into the cytoplasm, the activation of caspase and lipid peroxidation. All these alterations were significantly inhibited following treatment with resveratrol. The results demonstrated that resveratrol prevented HUVEC apoptosis through inhibiting mitochondria‑derived oxidative damage. These findings may provide a novel mechanism by which resveratrol prevents against endothelial cell apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Chen X and Li J: Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress. Mol Med Rep 15: 2457-2464, 2017.
APA
Liu, Y., Chen, X., & Li, J. (2017). Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress. Molecular Medicine Reports, 15, 2457-2464. https://doi.org/10.3892/mmr.2017.6304
MLA
Liu, Y., Chen, X., Li, J."Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress". Molecular Medicine Reports 15.5 (2017): 2457-2464.
Chicago
Liu, Y., Chen, X., Li, J."Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress". Molecular Medicine Reports 15, no. 5 (2017): 2457-2464. https://doi.org/10.3892/mmr.2017.6304
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Chen X and Li J: Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress. Mol Med Rep 15: 2457-2464, 2017.
APA
Liu, Y., Chen, X., & Li, J. (2017). Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress. Molecular Medicine Reports, 15, 2457-2464. https://doi.org/10.3892/mmr.2017.6304
MLA
Liu, Y., Chen, X., Li, J."Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress". Molecular Medicine Reports 15.5 (2017): 2457-2464.
Chicago
Liu, Y., Chen, X., Li, J."Resveratrol protects against oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cell apoptosis via inhibition of mitochondrial‑derived oxidative stress". Molecular Medicine Reports 15, no. 5 (2017): 2457-2464. https://doi.org/10.3892/mmr.2017.6304
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