Inhibitory effect of Angelica gigas on cold‑induced RhoA activation in vascular cells

Lee,Kangwook; Chae,Min Soo; Cho,Sung‑Gook; Go,Ho Yeon; Sun,Seung‑Ho; Jang,Junbock; Jung,Ki‑Yong; Choi,You‑Kyung; Song,Yun‑Kyung; Sim,Sung Yong; Lee,Hye Lim; Kang,Mi Suk; Jeon,Chan‑Yong; Ko,Seong Gyu

doi:10.3892/mmr.2017.6404

May-2017 Volume 15 Issue 5

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May-2017 Volume 15 Issue 5

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Article

Inhibitory effect of Angelica gigas on cold‑induced RhoA activation in vascular cells

Authors:
- Kangwook Lee
- Min Soo Chae
- Sung‑Gook Cho
- Ho Yeon Go
- Seung‑Ho Sun
- Junbock Jang
- Ki‑Yong Jung
- You‑Kyung Choi
- Yun‑Kyung Song
- Sung Yong Sim
- Hye Lim Lee
- Mi Suk Kang
- Chan‑Yong Jeon
- Seong Gyu Ko
View Affiliations / Copyright

Affiliations: Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02477, Republic of Korea, Department of Korean Medicine Obstetrics & Gynecology, Graduate School, Kyung Hee University, Seoul 02477, Republic of Korea, Department of Biotechnology, Korea National University of Transportation, Cheongju, Chungbuk 27469, Republic of Korea, Department of Korean Internal Medicine, College of Korean Medicine, Semyung University, Chungju, Chungbuk 27136, Republic of Korea, Department of Oriental Internal Medicine, College of Korean Medicine, Sangji University, Wonju, Gangwon 23339, Republic of Korea, Department of Korean Medicine Obstetrics and Gynecology, College of Korean Medicine, Kyung Hee University, Seoul 02477, Republic of Korea, Department of Korean Internal Medicine, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 13120, Republic of Korea, Department of Korean Rehabilitation Medicine, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 461701, Republic of Korea, Department of Korean Ophthalmology & Otolaryngology, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 461701, Republic of Korea, Department of Korean Pediatrics, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 461701, Republic of Korea, Department of Acupuncture and Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 461701, Republic of Korea, Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul 02453, Republic of Korea
Pages: 3143-3146
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Published online on: March 28, 2017

https://doi.org/10.3892/mmr.2017.6404
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Abstract

The herbal extract Angelica gigas (AG) has been applied as a vasodilating agent for patients suffering from vascular diseases for many years; however, the underlying mechanism has not been fully elucidated. The present study hypothesized that the anti‑vasoconstrictive effect of AG may be effective in the treatment of abnormal cold‑mediated vasospasms that occur in Raynaud's phenomenon (RP). The effect of AG on the activity of ras homolog gene family member A (RhoA) was investigated in cold‑exposed vascular cells. Vascular cells were pretreated to AG, followed by a warm (37˚C) or cold (25˚C) incubation for 30 min and investigated with western blotting, ELISA and confocal microscopy. Cold treatment induced the activation of RhoA in pericytes and vascular endothelial cells, however this was reduced by treatment with AG. Furthermore, AG treatment reduced the endothelin‑1 (ET‑1)‑mediated RhoA activation in pericytes; however, cold‑induced ET‑1 production by vascular endothelial cells was not affected by treatment with AG. In addition, AG treatment suppressed the formation of stress fibers and focal adhesion complexes, and the cold‑induced phosphorylation of focal adhesion kinase, proto‑oncogene tyrosine‑protein kinase Src and extracellular signal‑related kinase. Therefore, AG treatment demonstrated an ability to reduce cold‑induced RhoA activation in pericytes and vascular endothelial cells, and attenuated ET‑1‑mediated RhoA activation in pericytes. In conclusion, the present study indicated that AG may be useful for the treatment of RP.

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