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Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats

  • Authors:
    • Yuzhuo Yang
    • Zhe Zhang
    • Hongliang Zhang
    • Kai Hong
    • Wenhao Tang
    • Lianming Zhao
    • Haocheng Lin
    • Defeng Liu
    • Jiaming Mao
    • Han Wu
    • Hui Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Peking University Third Hospital, Beijing 100191, P.R. China, Reproductive Medicine Center, Peking University Third Hospital, Beijing 100191, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 491-498
    |
    Published online on: May 25, 2017
       https://doi.org/10.3892/mmr.2017.6624
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Abstract

Acrolein has been reported to have diverse toxic effects on various organs, including the reproductive system. However, little is known regarding the effects of maternal acrolein exposure on testicular steroidogenesis in male offspring. The present study investigated the effects of acrolein on fetal testosterone production and associated genes. Pregnant Sprague‑Dawley rats were intraperitoneally injected with vehicle (normal saline) or 1, 2 or 5 mg/kg acrolein from gestational day (GD) 14‑20, and fetal testes were examined on GD 21. Fetal body and testicular weights were markedly reduced in pups following exposure to high doses of acrolein (5 mg/kg) in late pregnancy. Notably, in utero exposure of 5 mg/kg acrolein significantly decreased the testicular testosterone level and downregulated the expression levels of steroidogenic acute regulatory protein (StAR) and 3β‑hydroxysteroid dehydrogenase (3β‑HSD), whereas the levels of other steroidogenic enzymes, including scavenger receptor class B, cholesterol side‑chain cleavage enzyme and steroid 17 alpha‑hydroxylase/17,20 lyase, were unaffected. Furthermore, the 3β‑HSD immunoreactive area in the interstitial region of the fetal testes was reduced at a 5 mg/kg dose, whereas the protein expression levels of 4‑hydroxynonenalwere dose‑dependently increased following maternal exposure to acrolein. mRNA expression levels of insulin‑like factor 3, a critical gene involved in testicular descent, were unaltered following maternal acrolein exposure. Taken together, the results of the present study suggested that maternal exposure to high doses of acrolein inhibited fetal testosterone synthesis, and abnormal expression of StAR and 3β‑HSD may be associated with impairment of the steroidogenic capacity.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Zhang Z, Zhang H, Hong K, Tang W, Zhao L, Lin H, Liu D, Mao J, Wu H, Wu H, et al: Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats. Mol Med Rep 16: 491-498, 2017.
APA
Yang, Y., Zhang, Z., Zhang, H., Hong, K., Tang, W., Zhao, L. ... Jiang, H. (2017). Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats. Molecular Medicine Reports, 16, 491-498. https://doi.org/10.3892/mmr.2017.6624
MLA
Yang, Y., Zhang, Z., Zhang, H., Hong, K., Tang, W., Zhao, L., Lin, H., Liu, D., Mao, J., Wu, H., Jiang, H."Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats". Molecular Medicine Reports 16.1 (2017): 491-498.
Chicago
Yang, Y., Zhang, Z., Zhang, H., Hong, K., Tang, W., Zhao, L., Lin, H., Liu, D., Mao, J., Wu, H., Jiang, H."Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats". Molecular Medicine Reports 16, no. 1 (2017): 491-498. https://doi.org/10.3892/mmr.2017.6624
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Zhang Z, Zhang H, Hong K, Tang W, Zhao L, Lin H, Liu D, Mao J, Wu H, Wu H, et al: Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats. Mol Med Rep 16: 491-498, 2017.
APA
Yang, Y., Zhang, Z., Zhang, H., Hong, K., Tang, W., Zhao, L. ... Jiang, H. (2017). Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats. Molecular Medicine Reports, 16, 491-498. https://doi.org/10.3892/mmr.2017.6624
MLA
Yang, Y., Zhang, Z., Zhang, H., Hong, K., Tang, W., Zhao, L., Lin, H., Liu, D., Mao, J., Wu, H., Jiang, H."Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats". Molecular Medicine Reports 16.1 (2017): 491-498.
Chicago
Yang, Y., Zhang, Z., Zhang, H., Hong, K., Tang, W., Zhao, L., Lin, H., Liu, D., Mao, J., Wu, H., Jiang, H."Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats". Molecular Medicine Reports 16, no. 1 (2017): 491-498. https://doi.org/10.3892/mmr.2017.6624
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