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Article

Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3

  • Authors:
    • Yangyang Han
    • Tianxiao Wang
    • Shudong Sun
    • Zhaohui Zhai
    • Shengjian Tang
  • View Affiliations / Copyright

    Affiliations: Plastic Surgery Institute, Weifang Medical University, Weifang, Shandong 261053, P.R. China, Department of Burns, Weifang People's Hospital, Weifang, Shandong 261053, P.R. China
  • Pages: 2856-2862
    |
    Published online on: July 4, 2017
       https://doi.org/10.3892/mmr.2017.6914
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Abstract

Forkhead box L2 (FOXL2) is a transcription factor, which is involved in blepharophimosis, ptosis, and epicanthus in versus syndrome (BPES), premature ovarian failure (POF), as well as almost all stages of ovarian development and function. FOXL2 has various target genes, which are implicated in numerous processes, including sex determination, cell cycle regulation and apoptosis and stress response regulation in mammals. However, studies regarding the upstream regulation of FOXL2 are limited. In the present study, the promoter of FOXL2 was successfully cloned and registered in Gen Bank, and a dual luciferase reporter (DLR) analysis demonstrated that the luciferase activity was significantly induced by the promoter of FOXL2. Subsequently, bioinformatics analysis indicated that FOXL2 may be regulated by STAT3, and this was confirmed by a DLR analysis and western blotting, using STAT3 inhibitors. Further study using real‑time cellular analysis indicated that the viability of He La cells was markedly suppressed by STAT3 inhibitors. The present study demonstrated novel findings regarding the upstream regulation of FOXL2 expression and provide a new perspective for future studies in the field.
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1 

Verdin H and De Baere E: FOXL2 impairment in human disease. Horm Res Paediatr. 77:2–11. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Rosario R, Cohen PA and Shelling AN: The role of FOXL2 in the pathogenesis of adult ovarian granulosa cell tumours. Gynecol Oncol. 133:382–387. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Georges A, Auguste A, Bessière L, Vanet A, Todeschini AL and Veitia RA: FOXL2: A central transcription factor of the ovary. J Mol Endocrinol. 52:R17–R33. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Cocquet J, Pailhoux E, Jaubert F, Servel N, Xia X, Pannetier M, De Baere E, Messiaen L, Cotinot C, Fellous M and Veitia RA: Evolution and expression of FOXL2. J Med Genet. 39:916–921. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Uhlenhaut NH, Jakob S, Anlag K, Eisenberger T, Sekido R, Kress J, Treier AC, Klugmann C, Klasen C, Holter NI, et al: Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. Cell. 139:1130–1142. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Garcia-Ortiz JE, Pelosi E, Omari S, Nedorezov T, Piao Y, Karmazin J, Uda M, Cao A, Cole SW, Forabosco A, et al: Foxl2 functions in sex determination and histogenesis throughout mouse ovary development. BMC Dev Biol. 9:362009. View Article : Google Scholar : PubMed/NCBI

7 

Qian X, Shu A, Qin W, Xing Q, Gao J, Yang J, Feng G and He L: A novel insertion mutation in the FOXL2 gene is detected in a big Chinese family with blepharophimosis-ptosis-epicanthus inversus. Mutat Res. 554:19–22. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Li WX, Wang XK, Sun Y, Wang YL, Lin LX and Tang SJ: A novel mutation in the FOXL2 gene in a Chinese family with blepharophimosis, ptosis, and epicanthus inversus syndrome. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 22:372–375. 2005.PubMed/NCBI

9 

Yu HC, Geiger EA, Medne L, Zackai EH and Shaikh TH: An individual with blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) and additional features expands the phenotype associated with mutations in KAT6B. Am J Med Genet A. 164A:950–957. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Duffin K, Bayne RA, Childs AJ, Collins C and Anderson RA: The forkhead transcription factor FOXL2 is expressed in somatic cells of the human ovary prior to follicle formation. Mol Hum Reprod. 15:771–777. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Caburet S, Georges A, LHôte D, Todeschini AL, Benayoun BA and Veitia RA: The transcription factor FOXL2: At the crossroads of ovarian physiology and pathology. Mol Cell Endocrinol. 356:55–64. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Auguste A, Chassot AA, Grégoire EP, Renault L, Pannetier M, Treier M, Pailhoux E and Chaboissier MC: Loss of R-spondin1 and Foxl2 amplifies female-to-male sex reversal in XX mice. Sex Dev. 5:304–317. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Dai A, Sun H, Fang T, Zhang Q, Wu S, Jiang Y, Ding L, Yan G and Hu Y: MicroRNA-133b stimulates ovarian estradiol synthesis by targeting Foxl2. FEBS Lett. 587:2474–2482. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Luo Y, Wu X, Ling Z, Yuan L, Cheng Y, Chen J and Xiang C: microRNA133a targets Foxl2 and promotes differentiation of C2C12 into myogenic progenitor cells. DNA Cell Biol. 34:29–36. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Eiring AM, Page BD, Kraft IL, Mason CC, Vellore NA, Resetca D, Zabriskie MS, Zhang TY, Khorashad JS, Engar AJ, et al: Combined STAT3 and BCR-ABL1 inhibition induces synthetic lethality in therapy-resistant chronic myeloid leukemia. Leukemia. 29:586–597. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Takeda K, Kaisho T, Yoshida N, Takeda J, Kishimoto T and Akira S: Correction: Stat3 activation is responsible for IL-6-dependent T cell proliferation through preventing apoptosis: Generation and characterization of T cell-specific Stat3-deficient mice. J Immunol. 194:35262015. View Article : Google Scholar : PubMed/NCBI

17 

Lee HJ, Zhuang G, Cao Y, Du P, Kim HJ and Settleman J: Drug resistance via feedback activation of Stat3 in oncogene-addicted cancer cells. Cancer Cell. 26:207–221. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Furqan M, Akinleye A, Mukhi N, Mittal V, Chen Y and Liu D: STAT inhibitors for cancer therapy. J Hematol Oncol. 6:902013. View Article : Google Scholar : PubMed/NCBI

19 

Wang T, Li F and Tang S: MiR-30a upregulates BCL2A1, IER3 and cyclin D2 expression by targeting FOXL2. Oncol Lett. 9:967–971. 2015.PubMed/NCBI

20 

Pisarska MD, Barlow G and Kuo FT: Minireview: Roles of the forkhead transcription factor FOXL2 in granulosa cell biology and pathology. Endocrinology. 152:1199–1208. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Kashimada K, Pelosi E, Chen H, Schlessinger D, Wilhelm D and Koopman P: FOXL2 and BMP2 act cooperatively to regulate follistatin gene expression during ovarian development. Endocrinology. 152:272–280. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Rosario R, Araki H, Print CG and Shelling AN: The transcriptional targets of mutant FOXL2 in granulosa cell tumours. PLoS One. 7:e462702012. View Article : Google Scholar : PubMed/NCBI

23 

Rosario R, Blenkiron C and Shelling AN: Comparative study of microRNA regulation on FOXL2 between adult-type and juvenile-type granulosa cell tumours in vitro. Gynecol Oncol. 129:209–215. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Tran S, Lamba P, Wang Y and Bernard DJ: SMADs and FOXL2 synergistically regulate murine FSHbeta transcription via a conserved proximal promoter element. Mol Endocrinol. 25:1170–1183. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Cheng JC, Klausen C and Leung PC: Overexpression of wild-type but not C134W mutant FOXL2 enhances GnRH-induced cell apoptosis by increasing GnRH receptor expression in human granulosa cell tumors. PLoS One. 8:e550992013. View Article : Google Scholar : PubMed/NCBI

26 

Benayoun BA, Batista F, Auer J, Dipietromaria A, LHôte D, De Baere E and Veitia RA: Positive and negative feedback regulates the transcription factor FOXL2 in response to cell stress: Evidence for a regulatory imbalance induced by disease-causing mutations. Hum Mol Genet. 18:632–644. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Staniszewska AD, Pensa S, Caffarel MM, Anderson LH, Poli V and Watson CJ: Stat3 is required to maintain the full differentiation potential of mammary stem cells and the proliferative potential of mammary luminal progenitors. PLoS One. 7:e526082012. View Article : Google Scholar : PubMed/NCBI

28 

Rokavec M, Öner MG, Li H, Jackstadt R, Jiang L, Lodygin D, Kaller M, Horst D, Ziegler PK, Schwitalla S, et al: IL-6R/STAT3/miR-34a feedback loop promotes EMT-mediated colorectal cancer invasion and metastasis. J Clin Invest. 124:1853–1867. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Iwamaru A, Szymanski S, Iwado E, Aoki H, Yokoyama T, Fokt I, Hess K, Conrad C, Madden T, Sawaya R, et al: A novel inhibitor of the STAT3 pathway induces apoptosis in malignant glioma cells both in vitro and in vivo. Oncogene. 26:2435–2444. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Daniel JM, Dutzmann J, Bielenberg W, Widmer-Teske R, Gündüz D, Hamm CW and Sedding DG: Inhibition of STAT3 signaling prevents vascular smooth muscle cell proliferation and neointima formation. Basic Res Cardiol. 107:2612012. View Article : Google Scholar : PubMed/NCBI

31 

Redell MS, Ruiz MJ, Alonzo TA, Gerbing RB and Tweardy DJ: Stat3 signaling in acute myeloid leukemia: Ligand-dependent and -independent activation and induction of apoptosis by a novel small-molecule Stat3 inhibitor. Blood. 117:5701–5709. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Gad SE and Sullivan DW: Dimethyl Sulfoxide (DMSO)Encyclopedia Toxicol. Wexler P: 3rd. Academic Press/Elsevier; London: pp. 166–168. 2014, View Article : Google Scholar

33 

Galvao J, Davis B, Tilley M, Normando E, Duchen MR and Cordeiro MF: Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J. 28:1317–1330. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Kim JH, Yoon S, Park M, Park HO, Ko JJ, Lee K and Bae J: Differential apoptotic activities of wild-type FOXL2 and the adult-type granulosa cell tumor-associated mutant FOXL2 (C134W). Oncogene. 30:1653–1663. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Han Y, Wang T, Sun S, Zhai Z and Tang S: Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Mol Med Rep 16: 2856-2862, 2017.
APA
Han, Y., Wang, T., Sun, S., Zhai, Z., & Tang, S. (2017). Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Molecular Medicine Reports, 16, 2856-2862. https://doi.org/10.3892/mmr.2017.6914
MLA
Han, Y., Wang, T., Sun, S., Zhai, Z., Tang, S."Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3". Molecular Medicine Reports 16.3 (2017): 2856-2862.
Chicago
Han, Y., Wang, T., Sun, S., Zhai, Z., Tang, S."Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3". Molecular Medicine Reports 16, no. 3 (2017): 2856-2862. https://doi.org/10.3892/mmr.2017.6914
Copy and paste a formatted citation
x
Spandidos Publications style
Han Y, Wang T, Sun S, Zhai Z and Tang S: Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Mol Med Rep 16: 2856-2862, 2017.
APA
Han, Y., Wang, T., Sun, S., Zhai, Z., & Tang, S. (2017). Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3. Molecular Medicine Reports, 16, 2856-2862. https://doi.org/10.3892/mmr.2017.6914
MLA
Han, Y., Wang, T., Sun, S., Zhai, Z., Tang, S."Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3". Molecular Medicine Reports 16.3 (2017): 2856-2862.
Chicago
Han, Y., Wang, T., Sun, S., Zhai, Z., Tang, S."Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3". Molecular Medicine Reports 16, no. 3 (2017): 2856-2862. https://doi.org/10.3892/mmr.2017.6914
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