Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells

Zhu,Jingling; Meng,Pingping; Wang,Qian; Wang,Haiyan; Zhang,Jinli; Li,Yuanyuan; Li,Dongzheng; Tan,Xiaohua; Yang,Lei; Huang,Jin

doi:10.3892/mmr.2017.6987

Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells

Authors:
- Jingling Zhu
- Pingping Meng
- Qian Wang
- Haiyan Wang
- Jinli Zhang
- Yuanyuan Li
- Dongzheng Li
- Xiaohua Tan
- Lei Yang
- Jin Huang
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Affiliations: The Key Laboratory of Xinjiang Endemic and Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China, Occupational and Environmental Health, Department of Preventive Medicine, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang 310036, P.R. China
Published online on: July 14, 2017 https://doi.org/10.3892/mmr.2017.6987
Pages: 3201-3207
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

While the neurotrophic factor neuritin is known to be involved in neurodevelopment, the effects of this compound on cell differentiation remain unclear. The present study demonstrated that neuritin treatment induced the differentiation of rat bone marrow‑derived mesenchymal stem cells (rBM‑MSCs) into neuron‑like (NL) cells. For these analyses, rBM‑MSCs were incubated with 0.5 µg/ml neuritin for 24 h. Following induction, 27% of the rBM‑MSCs exhibited typical NL cell morphologies. Subsequently, NL cells were characterized by examining the expression of neuronal markers and by analysis of cell functions. The findings demonstrated that the NL cells produced by neuritin treatment expressed the neuronal markers neuron‑specific enolase and microtubule associate protein 2, and secreted the neurotransmitter 5‑hydroxytryptamine. Furthermore, the NL cells exhibited certain partial neural‑electrophysiological functions. In conclusion, neuritin treatment may be an effective method for inducing the differentiation of BM‑MSCs towards NL cells. This may provide an alternative, potentially complementary tool for disease modeling and the development of cell‑based therapies.

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