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Article Open Access

Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine

  • Authors:
    • Shan Jiang
    • Song Zhang
  • View Affiliations / Copyright

    Affiliations: Cardiovascular Department, Xin Hua Hospital, Affiliated Hospital of Shanghai Jiaotong University, Shanghai 200092, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5887-5893
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/mmr.2017.7373
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Abstract

As a novel type of seed cell, amniotic fluid‑derived mesenchymal stem cells (AFMSCs) are promising for the regeneration of myocardial cells. A focus of cardiovascular regenerative medicine is to improve the efficiency of AFMSC differentiation. The present study replaced the traditional method of AFMSC differentiation with a combined induction method, in order to improve the efficiency of directional differentiation. AFMSCs were obtained from rabbit amniotic fluid samples, and western blot analysis was performed to analyze the expression of octamer‑binding transcription factor 4 (OCT4), and tumorigenicity experiments were conducted. AFMSCs were divided into the following 4 groups: Induction with transforming growth factor β1 (TGFβ1); induction with 5‑azacytidine (5Aza); induction with TGFβ1 and 5Aza combined; and untreated controls. Reverse transcription‑quantitative polymerase chain reaction was performed to analyze the expression of cardiac‑specific GATA binding protein 4 (GATA4), and immunofluorescence was employed to analyze the expression of cardiac troponin T (cTnT). In addition, western blotting was performed to analyze the expression of connexin 43, and transmission electron microscopy was used to observe the ultrastructure of the differentiated cells. AFMSCs exhibited positive OCT4 expression and were not observed to induce tumor development in nude mice. The expression levels of GATA4, cTnT, and connexin 43 in the combined induction group were markedly higher when compared with the remaining groups. Transmission electron microscopy analysis revealed that differentiated cells exhibited myocardial cell characteristics. In conclusion, AFMSCs are multipotent, non‑tumorigenic cells that are capable of differentiating into cardiomyocyte‑like cells. This combined induction method may improve the efficiency of directed differentiation.
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1 

Zhao L and Hantash BM: TGF-β1 regulates differentiation of bone marrow mesenchymal stem cells. Vitam Horm. 87:127–141. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Mohanty S, Bose S, Jain KG, Bhargava B and Airan B: TGFβ1 contributes to cardiomyogenic-like differentiation of human bone marrow mesenchymal stem cells. Int J Cardiol. 163:93–99. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Zhang S, Liu X, Goldstein S, Li Y, Ge J, He B, Fei X, Wang Z and Ruiz G: Role of the JAK/STAT signaling pathway in the pathogenesis of acute myocardial infarction in rats and its effect on NF-κB expression. Mol Med Rep. 7:93–98. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Chiavegato A, Bollini S, Pozzobon M, Callegari A, Gasparotto L, Taiani J, Piccoli M, Lenzini E, Gerosa G, Vendramin I, et al: Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat. J Mol Cell Cardiol. 42:746–759. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Benthien JP and Behrens P: Reviewing subchondral cartilage surgery: Considerations for standardised and outcome predictable cartilage remodelling: A technical note. Int Orthop. 37:2139–2145. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Fei X, Jiang S, Zhang S, Li Y, Ge J, He B, Goldstein S and Ruiz G: Isolation, culture and identification of amniotic fluid-derived mesenchymal stem cells. Cell Biochem Biophys. 67:689–694. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, et al: Bone marrow cells regenerate infarcted myocardium. Nature. 410:701–705. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Dubuis C, May L, Alonso F, Luca L, Mylonaki I, Meda P, Delie F, Jordan O, Déglise S, Corpataux JM, et al: Atorvastatin-loaded hydrogel affects the smooth muscle cells of human veins. J Pharmacol Exp Ther. 347:574–581. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Guan X, Delo DM, Atala A and Soker S: In vitro cardiomyogenic potential of human amniotic fluid stem cells. J Tissue Eng Regen Med. 5:220–228. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Aseem O, Barth JL, Klatt SC, Smith BT and Argraves WS: Cubilin expression is monoallelic and epigenetically augmented via PPARs. BMC Genomics. 14:4052013. View Article : Google Scholar : PubMed/NCBI

12 

Xing Y, Lv A, Wang L and Yan X: The combination of angiotensin II and 5-azacytidine promotes cardiomyocyte differentiation of rat bone marrow mesenchymal stem cells. Mol Cell Biochem. 360:279–287. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Balana B, Nicoletti C, Zahanich I, Graf EM, Christ T, Boxberger S and Ravens U: 5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells. Cell Res. 16:949–960. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Zhang Y, Chu Y, Shen W and Dou Z: Effect of 5-azacytidine induction duration on differentiation of human first-trimester fetal mesenchymal stem cells towards cardiomyocyte-like cells. Interact Cardiovasc Thorac Surg. 9:943–946. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Kim YM, Kim J, Heo SC, Shin SH, Do EK, Suh DS, Kim KH, Yoon MS, Lee TG and Kim JH: Proteomic identification of ADAM12 as a regulator for TGF-β1-induced differentiation of human mesenchymal stem cells to smooth muscle cells. PLoS One. 7:e408202012. View Article : Google Scholar : PubMed/NCBI

16 

Chen LX, Yang K, Sun M, Chen Q, Wang ZH, Hu GY and Tao LJ: Fluorofenidone inhibits transforming growth factor-beta1-induced cardiac myofibroblast differentiation. Pharmazie. 67:452–456. 2012.PubMed/NCBI

17 

Huntgeburth M, Tiemann K, Shahverdyan R, Schlüter KD, Schreckenberg R, Gross ML, Mödersheim S, Caglayan E, Müller-Ehmsen J, Ghanem A, et al: Transforming growth factor β1 oppositely regulates the hypertrophic and contractile response to β-adrenergic stimulation in the heart. PLoS One. 6:e266282011. View Article : Google Scholar : PubMed/NCBI

18 

Wan Safwani WK, Makpol S, Sathapan S and Chua KH: 5-Azacytidine is insufficient for cardiogenesis in human adipose-derived stem cells. J Negat Results Biomed. 11:32012. View Article : Google Scholar : PubMed/NCBI

19 

Rosca AM and Burlacu A: Effect of 5-azacytidine: Evidence for alteration of the multipotent ability of mesenchymal stem cells. Stem Cells Dev. 20:1213–1221. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Zhang S, Zhang M, Goldstein S, Li Y, Ge J, He B and Ruiz G: The effect of c-fos on acute myocardial infarction and the significance of metoprolol intervention in a rat model. Cell Biochem Biophys. 65:249–255. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Xu M, Millard RW and Ashraf M: Role of GATA-4 in differentiation and survival of bone marrow mesenchymal stem cells. Prog Mol Biol Transl Sci. 111:217–241. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Wada R, Muraoka N, Inagawa K, Yamakawa H, Miyamoto K, Sadahiro T, Umei T, Kaneda R, Suzuki T, Kamiya K, et al: Induction of human cardiomyocyte-like cells from fibroblasts by defined factors. Proc Natl Acad Sci USA. 110:12667–12672. 2013; View Article : Google Scholar : PubMed/NCBI

23 

de Lemos JA: Increasingly sensitive assays for cardiac troponins: A review. JAMA. 309:2262–2269. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Wildi K, Reichlin T, Twerenbold R, Mäder F, Zellweger C, Moehring B, Stallone F, Minners J, Gimenez M Rubini, et al: Serial changes in high-sensitivity cardiac troponin I in the early diagnosis of acute myocardial infarction. Int J Cardiol. 168:4103–4110. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Delmar M and Makita N: Cardiac connexins, mutations and arrhythmias. Curr Opin Cardiol. 27:236–241. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Thimm J, Mechler A, Lin H, Rhee S and Lal R: Calcium-dependent open/closed conformations and interfacial energy maps of reconstituted hemichannels. J Biol Chem. 280:10646–10654. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Antunes E, Borrecho G, Oliveira P, Brito J, Águas A and dos Santos J Martins: Immunohistochemical evaluation of cardiac connexin43 in rats exposed to low-frequency noise. Int J Clin Exp Pathol. 6:1874–1879. 2013.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jiang S and Zhang S: Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine. Mol Med Rep 16: 5887-5893, 2017.
APA
Jiang, S., & Zhang, S. (2017). Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine. Molecular Medicine Reports, 16, 5887-5893. https://doi.org/10.3892/mmr.2017.7373
MLA
Jiang, S., Zhang, S."Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine". Molecular Medicine Reports 16.5 (2017): 5887-5893.
Chicago
Jiang, S., Zhang, S."Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine". Molecular Medicine Reports 16, no. 5 (2017): 5887-5893. https://doi.org/10.3892/mmr.2017.7373
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang S and Zhang S: Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine. Mol Med Rep 16: 5887-5893, 2017.
APA
Jiang, S., & Zhang, S. (2017). Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine. Molecular Medicine Reports, 16, 5887-5893. https://doi.org/10.3892/mmr.2017.7373
MLA
Jiang, S., Zhang, S."Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine". Molecular Medicine Reports 16.5 (2017): 5887-5893.
Chicago
Jiang, S., Zhang, S."Differentiation of cardiomyocytes from amniotic fluid‑derived mesenchymal stem cells by combined induction with transforming growth factor β1 and 5‑azacytidine". Molecular Medicine Reports 16, no. 5 (2017): 5887-5893. https://doi.org/10.3892/mmr.2017.7373
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