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Article

Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

  • Authors:
    • Naoki Nanashima
    • Kayo Horie
    • Mitsuru Chiba
    • Manabu Nakano
    • Hayato Maeda
    • Toshiya Nakamura
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori 036‑8561, Japan
  • Pages: 6134-6141
    |
    Published online on: August 29, 2017
       https://doi.org/10.3892/mmr.2017.7391
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Abstract

Blackcurrants (Ribes nigrum L., Grossulariaceae) possess a high content of anthocyanin polyphenols, which have been demonstrated to exhibit beneficial effects on health due to their antioxidant and anticarcinogenic prope­rties. The present study investigated novel functions of anthocyanin‑rich blackcurrant extracts (BCEs) in a healthy mammary epithelial cell line, MCF10A. The percentages of viable cells were 85, 75, 53 and 31% following exposure to 50, 100, 200 and 400 µg/ml BCE, respectively. The half‑maximal response concentration of BCE was 237.7 µg/ml. Microarray and Ingenuity® Pathway Analysis demonstrated that BCE downregulated cell cycle signaling, including upstream genes with mitotic roles such as polo‑like kinase signaling. BCE increased the number of cells in the G0/G1 phase and decreased the number of cells in the S and G2/M phases. Alkaline comet assays demonstrated that 50 and 100 µg/ml BCE induced DNA damage in a dose‑dependent manner. Cultures treated with 0, 50, and 100 µg/ml BCE contained 4.6, 13.4 and 16.0% apoptotic cells, respectively. As compared with the untreated cultures (1.9%), the number of necrotic cells increased in the 100 µg/ml BCE‑treated cultures (from 1.9 to 4.3%) but not in the 50 µg/ml BCE‑treated cultures. Reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that BCE reduced mRNA expression of the genomic caretaker lysine‑specific demethylas  5B (KDM5B). The results suggested that blackcurrant anthocyanins may act as cell arrest and death inducers via KDM5B downregulation in healthy breast cells.
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1 

Delazar A, Khodaie L, Afshar J, Nahar L and Sarker SD: Isolation and free-radical-scavenging properties of cyanidin 3-O-glycosides from the fruits of Ribes biebersteinii. Berl. Acta Pharm. 60:1–11. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Gopalan A, Reuben SC, Ahmed S, Darvesh AS, Hohmann J and Bishayee A: The health benefits of blackcurrants. Food Funct. 3:795–809. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Määttä KR, Kamal-Eldin A and Törrönen AR: High-performance liquid chromatography (HPLC) analysis of phenolic compounds in berries with diode array and electrospray ionization mass spectrometric (MS) detection: Ribes species. J Agric Food Chem. 51:6736–6744. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Nielsen IL, Haren GR, Magnussen EL, Dragsted LO and Rasmussen SE: Quantification of anthocyanins in commercial black currant juices by simple high-performance liquid chromatography. Investigation of their pH stability and antioxidative potency. J Agric Food Chem. 51:5861–5866. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Matsumoto H, Takenami E, Iwasaki-Kurashige K, Osada T, Katsumura T and Hamaoka T: Effects of blackcurrant anthocyanin intake on peripheral muscle circulation during typing work in humans. Eur J Appl Physiol. 94:36–45. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Zhu Y, Xia M, Yang Y, Liu F, Li Z, Hao Y, Mi M, Jin T and Ling W: Purified anthocyanin supplementation improves endothelial function via NO-cGMP activation in hypercholesterolemic individuals. Clin Chem. 57:1524–1533. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Yamamoto A, Nakashima K, Kawamorita S, Sugiyama A, Miura M, Kamitai Y and Kato Y: Protective effects of raw and cooked blackcurrant extract on DNA damage induced by hydrogen peroxide in human lymphoblastoid cells. Pharm Biol. 52:782–788. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Aiyer HS, Warri AM, Woode DR, Hilakivi-Clarke L and Clarke R: Influence of berry polyphenols on receptor signaling and cell-death pathways: Implications for breast cancer prevention. J Agric Food Chem. 60:5693–5708. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Boivin D, Blanchette M, Barrette S, Moghrabi A and Beliveau R: Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NFkappaB by edible berry juice. Anticancer Res. 27:937–948. 2007.PubMed/NCBI

10 

Olsson ME, Gustavsson KE, Andersson S, Nilsson A and Duan RD: Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels. J Agric Food Chem. 52:7264–7271. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Catchpole S, Spencer-Dene B, Hall D, Santangelo S, Rosewell I, Guenatri M, Beatson R, Scibetta AG, Burchell JM and Taylor-Papadimitriou J: PLU-1/JARID1B/KDM5B is required for embryonic survival and contributes to cell proliferation in the mammary gland and in ER+ breast cancer cells. Int J Oncol. 38:1267–1277. 2011.PubMed/NCBI

12 

Li X, Liu L, Yang S, Song N, Zhou X, Gao J, Yu N, Shan L, Wang Q, Liang J, et al: Histone demethylase KDM5B is a key regulator of genome stability. Proc Natl Acad Sci USA. 111:7096–7101. 2014; View Article : Google Scholar : PubMed/NCBI

13 

Nanashima N, Horie K, Tomisawa T, Chiba M, Nakano M, Fujita T, Maeda H, Kitajima M, Takamagi S, Uchiyama D, et al: Phytoestrogenic activity of blackcurrant (Ribes nigrum) anthocyanins is mediated through estrogen receptor alpha. Mol Nutr Food Res. 59:2419–2431. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Nanashima N, Yamada T, Shimizu T and Tsuchida S: Deletion of phospholipase A2 group IVc induces apoptosis in rat mammary tumour cells by the nuclear factor-κB/lipocalin 2 pathway. Biochem J. 469:315–324. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Lin CJ, Chang YA, Lin YL, Liu SH, Chang CK and Chen RM: Preclinical effects of honokiol on treating glioblastoma multiforme via G1 phase arrest and cell apoptosis. Phytomedicine. 23:517–527. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Lu JJ, Cai YJ and Ding J: Curcumin induces DNA damage and caffeine-insensitive cell cycle arrest in colorectal carcinoma HCT116 cells. Mol Cell Biochem. 354:247–252. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Ouyang G, Yao L, Ruan K, Song G, Mao Y and Bao S: Genistein induces G2/M cell cycle arrest and apoptosis of human ovarian cancer cells via activation of DNA damage checkpoint pathways. Cell Biol Int. 33:1237–1244. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Zhang Z, Wang CZ, Du GJ, Qi LW, Calway T, He TC, Du W and Yuan CS: Genistein induces G2/M cell cycle arrest and apoptosis via ATM/p53-dependent pathway in human colon cancer cells. Int J Oncol. 43:289–296. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Wu QK, Koponen JM, Mykkanen HM and Törrönen AR: Berry phenolic extracts modulate the expression of p21 (WAF1) and Bax but not Bcl-2 in HT-29 colon cancer cells. J Agric Food Chem. 55:1156–1163. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Bishayee A, Haznagy-Radnai E, Mbimba T, Sipos P, Morazzoni P, Darvesh AS, Bhatia D and Hohmann J: Anthocyanin-rich black currant extract suppresses the growth of human hepatocellular carcinoma cells. Nat Prod Commun. 5:1613–1618. 2010.PubMed/NCBI

22 

Strebhardt K: Multifaceted polo-like kinases: Drug targets and antitargets for cancer therapy. Nat Rev Drug Discov. 9:643–660. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Ng WT Weng, Shin JS, Roberts TL, Wang B and Lee CS: Molecular interactions of polo-like kinase 1 in human cancers. J Clin Pathol. 69:557–562. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Asteriti IA, De Mattia F and Guarguaglini G: Cross-talk between AURKA and Plk1 in mitotic entry and spindle assembly. Front Oncol. 5:2832015. View Article : Google Scholar : PubMed/NCBI

25 

Guo D, Wang J, Wang X, Luo H, Zhang H, Cao D, Chen L and Huang N: Double directional adjusting estrogenic effect of naringin from Rhizoma drynariae (Gusuibu). J Ethnopharmacol. 138:451–457. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Lee YM, Kim JB, Bae JH, Lee JS, Kim PS, Jang HH and Kim HR: Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. in MCF-7 cells. BMC Complement Altern Med. 12:2602012. View Article : Google Scholar : PubMed/NCBI

27 

Limer JL and Speirs V: Phyto-oestrogens and breast cancer chemoprevention. Breast Cancer Res. 6:119–127. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Mahmoud AM, Yang W and Bosland MC: Soy isoflavones and prostate cancer: A review of molecular mechanisms. J Steroid Biochem Mol Biol. 140:116–132. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Li T, Zhu J, Guo L, Shi X, Liu Y and Yang X: Differential effects of polyphenols-enriched extracts from hawthorn fruit peels and fleshes on cell cycle and apoptosis in human MCF-7 breast carcinoma cells. Food Chem. 141:1008–1018. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Rice S and Whitehead SA: Phytoestrogens oestrogen synthesis and breast cancer. J Steroid Biochem Mol Biol. 108:186–195. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Seo HS, Ju JH, Jang K and Shin I: Induction of apoptotic cell death by phytoestrogens by up-regulating the levels of phospho-p53 and p21 in normal and malignant estrogen receptor α-negative breast cells. Nutr Res. 31:139–146. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Prasad R and Katiyar SK: Polyphenols from green tea inhibit the growth of melanoma cells through inhibition of class I histone deacetylases and induction of DNA damage. Genes Cancer. 6:49–61. 2015.PubMed/NCBI

33 

Demoulin B, Hermant M, Castrogiovanni C, Staudt C and Dumont P: Resveratrol induces DNA damage in colon cancer cells by poisoning topoisomerase II and activates the ATM kinase to trigger p53-dependent apoptosis. Toxicol In Vitro. 29:1156–1165. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Yamane K, Tateishi K, Klose RJ, Fang J, Fabrizio LA, Erdjument-Bromage H, Taylor-Papadimitriou J, Tempst P and Zhang Y: PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation. Mol Cell. 25:801–812. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Zou MR, Cao J, Liu Z, Huh SJ, Polyak K and Yan Q: Histone demethylase jumonji AT-rich interactive domain 1B (JARID1B) controls mammary gland development by regulating key developmental and lineage specification genes. J Biol Chem. 289:17620–17633. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Barrett KL, Demiranda D and Katula KS: Cyclin b1 promoter activity and functional cdk1 complex formation in G1 phase of human breast cancer cells. Cell Biol Int. 26:19–28. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Chang DC, Xu N and Luo KQ: Degradation of cyclin B is required for the onset of anaphase in Mammalian cells. J Biol Chem. 278:37865–37873. 2003. View Article : Google Scholar : PubMed/NCBI

38 

Ducommun B, Brambilla P, Félix MA, Franza BR Jr, Karsenti E and Draetta G: cdc2 phosphorylation is required for its interaction with cyclin. EMBO J. 10:3311–3319. 1991.PubMed/NCBI

39 

Scibetta AG, Santangelo S, Coleman J, Hall D, Chaplin T, Copier J, Catchpole S, Burchell J and Taylor-Papadimitriou J: Functional analysis of the transcription repressor PLU-1/JARID1B. Mol Cell Biol. 27:7220–7235. 2007. View Article : Google Scholar : PubMed/NCBI

40 

Mitra D, Das PM, Huynh FC and Jones FE: Jumonji/ARID1 B (JARID1B) protein promotes breast tumor cell cycle progression through epigenetic repression of microRNA let-7e. J Biol Chem. 286:40531–40535. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Nanashima N, Horie K, Chiba M, Nakano M, Maeda H and Nakamura T: Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis. Mol Med Rep 16: 6134-6141, 2017.
APA
Nanashima, N., Horie, K., Chiba, M., Nakano, M., Maeda, H., & Nakamura, T. (2017). Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis. Molecular Medicine Reports, 16, 6134-6141. https://doi.org/10.3892/mmr.2017.7391
MLA
Nanashima, N., Horie, K., Chiba, M., Nakano, M., Maeda, H., Nakamura, T."Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis". Molecular Medicine Reports 16.5 (2017): 6134-6141.
Chicago
Nanashima, N., Horie, K., Chiba, M., Nakano, M., Maeda, H., Nakamura, T."Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis". Molecular Medicine Reports 16, no. 5 (2017): 6134-6141. https://doi.org/10.3892/mmr.2017.7391
Copy and paste a formatted citation
x
Spandidos Publications style
Nanashima N, Horie K, Chiba M, Nakano M, Maeda H and Nakamura T: Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis. Mol Med Rep 16: 6134-6141, 2017.
APA
Nanashima, N., Horie, K., Chiba, M., Nakano, M., Maeda, H., & Nakamura, T. (2017). Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis. Molecular Medicine Reports, 16, 6134-6141. https://doi.org/10.3892/mmr.2017.7391
MLA
Nanashima, N., Horie, K., Chiba, M., Nakano, M., Maeda, H., Nakamura, T."Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis". Molecular Medicine Reports 16.5 (2017): 6134-6141.
Chicago
Nanashima, N., Horie, K., Chiba, M., Nakano, M., Maeda, H., Nakamura, T."Anthocyanin‑rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis". Molecular Medicine Reports 16, no. 5 (2017): 6134-6141. https://doi.org/10.3892/mmr.2017.7391
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