Open Access

miR‑21 attenuates contrast‑induced renal cell apoptosis by targeting PDCD4

  • Authors:
    • Kun Wang
    • Wei‑Jie Bei
    • Yuan‑Hui Liu
    • Hua‑Long Li
    • Shi‑Qun Chen
    • Kai‑Yang Lin
    • Zhi‑Ling Zhou
    • Ji‑Yan Chen
    • Yong Liu
    • Ning Tan
  • View Affiliations

  • Published online on: September 5, 2017     https://doi.org/10.3892/mmr.2017.7426
  • Pages: 6757-6763
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Contrast medium (CM) is widely used in cardiac catheterization; however, it may induce acute kidney injury or renal failure, although the underlying mechanism remains to be elucidated. MicroRNA‑21 (miR‑21) is involved in renal disease and has been indicated to regulate cellular apoptosis and fibrosis, although its role in CM‑induced renal cell injury is unknown. The present study examined the expression and potential targets of miR‑21 in human renal proximal tubular epithelial (HK‑2) cells following CM treatment. CM induced renal cell apoptosis and decreased miR‑21 expression. The expression level of the apoptosis regulator protein, B‑cell lymphoma 2 (Bcl‑2) was upregulated, whereas that of the apoptosis regulator, Bcl‑2‑associated X protein (Bax) was downregulated upon transfection of miR‑21 mimics; miR‑21 overexpression additionally directly inhibited the expression of programmed cell death protein 4 (PDCD4), as determined by a dual luciferase reporter assay, and PDCD4 silencing reduced the rate of HK‑2 cell apoptosis. The results of the present study indicated that miR‑21 protected renal cells against CM‑induced apoptosis by regulating PDCD4 expression.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang K, Bei WJ, Liu YH, Li HL, Chen SQ, Lin KY, Zhou ZL, Chen JY, Liu Y, Tan N, Tan N, et al: miR‑21 attenuates contrast‑induced renal cell apoptosis by targeting PDCD4. Mol Med Rep 16: 6757-6763, 2017
APA
Wang, K., Bei, W., Liu, Y., Li, H., Chen, S., Lin, K. ... Tan, N. (2017). miR‑21 attenuates contrast‑induced renal cell apoptosis by targeting PDCD4. Molecular Medicine Reports, 16, 6757-6763. https://doi.org/10.3892/mmr.2017.7426
MLA
Wang, K., Bei, W., Liu, Y., Li, H., Chen, S., Lin, K., Zhou, Z., Chen, J., Liu, Y., Tan, N."miR‑21 attenuates contrast‑induced renal cell apoptosis by targeting PDCD4". Molecular Medicine Reports 16.5 (2017): 6757-6763.
Chicago
Wang, K., Bei, W., Liu, Y., Li, H., Chen, S., Lin, K., Zhou, Z., Chen, J., Liu, Y., Tan, N."miR‑21 attenuates contrast‑induced renal cell apoptosis by targeting PDCD4". Molecular Medicine Reports 16, no. 5 (2017): 6757-6763. https://doi.org/10.3892/mmr.2017.7426