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Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism

  • Authors:
    • Yu Yang
    • Jicheng Tantai
    • Yifeng Sun
    • Chenxi Zhong
    • Zhigang Li
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6483-6488
    |
    Published online on: September 11, 2017
       https://doi.org/10.3892/mmr.2017.7453
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Abstract

Hyperoside (HY) is a major pharmacologically active component from Prunella vulgaris L. and Hypericum perforatum. The present study aimed to determine the anticancer effect of HY and determine the underlying mechanisms involved. Human A549 cells were treated with HY (10, 50 and 100 µM), and cell viability was detected by an MTT assay. Cell apoptosis and mitochondrial membrane potential were determined by flow cytometry. Western blot analysis was used to identify the expression of apoptosis‑associated proteins and phosphorylation of MAPK. The present study demonstrated that HY significantly inhibited the viability of A549 cells in a time‑ and dose‑dependent manner, and enhanced the percentage of apoptotic cells. HY also significantly increased the protein phosphorylation of p38 mitogen‑activated protein kinase (MAPK) and c‑Jun N‑terminal kinase (JNK), disrupted mitochondrial membrane penetrability, and triggered the release of mitochondrial cytochrome c and apoptosis‑inducing factor into the cytosol. Treatment with HY also activated the expression of caspase‑9 and caspase‑3. These results suggested that HY‑induced apoptosis was associated with activation of the p38 MAPK‑ and JNK‑induced mitochondrial death pathway. HY may offer potential for clinical applications in treating human non‑small cell lung cancer and improving cancer chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Tantai J, Sun Y, Zhong C and Li Z: Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism. Mol Med Rep 16: 6483-6488, 2017.
APA
Yang, Y., Tantai, J., Sun, Y., Zhong, C., & Li, Z. (2017). Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism. Molecular Medicine Reports, 16, 6483-6488. https://doi.org/10.3892/mmr.2017.7453
MLA
Yang, Y., Tantai, J., Sun, Y., Zhong, C., Li, Z."Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism". Molecular Medicine Reports 16.5 (2017): 6483-6488.
Chicago
Yang, Y., Tantai, J., Sun, Y., Zhong, C., Li, Z."Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism". Molecular Medicine Reports 16, no. 5 (2017): 6483-6488. https://doi.org/10.3892/mmr.2017.7453
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Tantai J, Sun Y, Zhong C and Li Z: Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism. Mol Med Rep 16: 6483-6488, 2017.
APA
Yang, Y., Tantai, J., Sun, Y., Zhong, C., & Li, Z. (2017). Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism. Molecular Medicine Reports, 16, 6483-6488. https://doi.org/10.3892/mmr.2017.7453
MLA
Yang, Y., Tantai, J., Sun, Y., Zhong, C., Li, Z."Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism". Molecular Medicine Reports 16.5 (2017): 6483-6488.
Chicago
Yang, Y., Tantai, J., Sun, Y., Zhong, C., Li, Z."Effect of hyperoside on the apoptosis of A549 human non‑small cell lung cancer cells and the underlying mechanism". Molecular Medicine Reports 16, no. 5 (2017): 6483-6488. https://doi.org/10.3892/mmr.2017.7453
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