MicroRNA‑138‑5p regulates neural stem cell proliferation and differentiation in vitro by targeting TRIP6 expression

Wang,Juan; Li,Jixia; Yang,Jian; Zhang,Lianguo; Gao,Shane; Jiao,Fei; Yi,Maoli; Xu,Jun

doi:10.3892/mmr.2017.7504

November-2017 Volume 16 Issue 5

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November-2017 Volume 16 Issue 5

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Article Open Access

MicroRNA‑138‑5p regulates neural stem cell proliferation and differentiation in vitro by targeting TRIP6 expression

Authors:
- Juan Wang
- Jixia Li
- Jian Yang
- Lianguo Zhang
- Shane Gao
- Fei Jiao
- Maoli Yi
- Jun Xu
View Affiliations / Copyright

Affiliations: Stem Cell Center, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China, Clinical Laboratory, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China, Administration Office, Yantai Blood Station, Yantai, Shandong 264000, P.R. China, Department of Thoracic Surgery, Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong 256603, P.R. China, Department of Biotechnology and Molecular, Binzhou Medical College, Yantai, Shandong 264003, P.R. China, Laboratory of Yantai Yuhuangding Hospital, Yantai, Shandong 264003, P.R. China

Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 7261-7266
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Published online on: September 18, 2017

https://doi.org/10.3892/mmr.2017.7504
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Abstract

Research on neural stem cells (NSCs) has recently focused on microRNAs (miRNAs), a class of small non‑coding RNAs that have crucial roles in regulating NSC proliferation and differentiation. In the present study, a quantitative‑polymerase chain reaction assay revealed that the expression of miRNA (miR)‑138‑5p was significantly decreased during neural differentiation of NSCs in vitro. Overexpression of miR‑138‑5p reduced NSC proliferation and increased NSC differentiation. Furthermore, suppression of miR‑138‑5p via transfection with a miRNA inhibitor enhanced NSC proliferation and attenuated NSC differentiation. Additionally, expression of thyroid hormone receptor interacting protein 6 (TRIP6), a critical regulator of NSCs, was negatively correlated with the miR‑138‑5p level. A luciferase assay demonstrated that miR‑138‑5p regulate TRIP6 by directly binding the 3'‑untranslated region of the mRNA. Additionally, upregulation of TRIP6 rescued the NSC proliferation deficiency induced by miR‑138‑5p and abolished miR‑138‑5p‑promoted NSCs differentiation. By contrast, downregulation of TRIP6 produced the opposite effect on proliferation and differentiation of NSCs transfected with anti‑miR‑138‑5p. Taken together, the data suggest that miR‑138‑5p regulates NSCs proliferation and differentiation, and may be useful in developing novel treatments for neurological disorders via manipulation of miR‑138‑5p in NSCs.

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