Gallic acid inhibits the growth of calf pulmonary arterial endothelial cells through cell death and glutathione depletion

  • Authors:
    • Woo Hyun Park
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  • Published online on: September 21, 2017     https://doi.org/10.3892/mmr.2017.7585
  • Pages: 7805-7812
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Abstract

Gallic acid (GA) exhibits a number of cellular effects, including apoptosis, which is associated with oxidative stress. The present study investigated the effects of GA on calf pulmonary arterial endothelial cell (CPAEC) growth and death, along with the levels of reactive oxygen species (ROS) and glutathione (GSH). GA treatment inhibited the growth of CPAECs at 24 h, and the half‑maximal inhibitory concentration (IC50) value of GA was ~30 µM. GA treatment also induced cell death, which was accompanied by a loss of mitochondrial membrane potential (ΔѰm). GA treatment in CPAECs resulted in decreased ROS levels, including O2•‑, whereas the number of GSH‑depleted cells increased. Neither a pan‑caspase inhibitor (benzyloxycarbonyl‑Val‑Ala‑Asp‑fluoromethylketone) nor buthionine sulfoximine treatment affected GA‑induced cell growth inhibition, cell death, ROS and GSH levels in CPAECs, whereas co‑treatment with N‑acetyl‑cysteine (NAC) resulted in enhanced cell growth inhibition, cell death and ΔѰm loss in these cells. Although NAC treatment did not significantly influence ROS levels in GA‑treated CPAECs, it significantly enhanced GSH depletion in these cells. In conclusion, GA inhibited the growth of CPAECs via cell death, which was associated with GSH depletion rather than alterations to ROS levels.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Park WH: Gallic acid inhibits the growth of calf pulmonary arterial endothelial cells through cell death and glutathione depletion. Mol Med Rep 16: 7805-7812, 2017
APA
Park, W.H. (2017). Gallic acid inhibits the growth of calf pulmonary arterial endothelial cells through cell death and glutathione depletion. Molecular Medicine Reports, 16, 7805-7812. https://doi.org/10.3892/mmr.2017.7585
MLA
Park, W. H."Gallic acid inhibits the growth of calf pulmonary arterial endothelial cells through cell death and glutathione depletion". Molecular Medicine Reports 16.5 (2017): 7805-7812.
Chicago
Park, W. H."Gallic acid inhibits the growth of calf pulmonary arterial endothelial cells through cell death and glutathione depletion". Molecular Medicine Reports 16, no. 5 (2017): 7805-7812. https://doi.org/10.3892/mmr.2017.7585