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Article

Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones

  • Authors:
    • Xiao Du
    • Yanhui Cai
    • Wenjin Xi
    • Rui Zhang
    • Lintao Jia
    • Angang Yang
    • Jing Zhao
    • Bo Yan
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 307-314
    |
    Published online on: October 20, 2017
       https://doi.org/10.3892/mmr.2017.7854
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Abstract

The functional influence of microRNA (miRNA)backbone selection remains unclear with respect to multiplexing miRNA‑based short hairpin RNAs (shRNAmiRs), due to a lack of comparative studies. To this end, a pair of shRNAmiR tetramers were designed in the present study that targeted four genes with a shared miR30a backbone (homo‑BB) or four miRNA backbones (hetero‑BB). A PBLT+ 293A cell line overexpressing four targets was established, which permitted simultaneous dissection of individual gene knockdown. Multi‑target inhibition was confirmed by a decrease in positive cell populations of the relative gene and mean fluorescence intensities, with almost comparable activities of homo‑ and hetero‑BB tetramers. Of note, this multi‑inhibition was sustained over a 1‑month period, with no notable difference, particularly in the late‑phased inhibitory effects between homo‑ and hetero‑BB tetra‑shRNA miRs. These preliminary data may indicate little influence of scaffold substitution in the functionalities of multiplexed shRNAmiRs and little recombination‑depleted risk of repetitively adopting the same miRNA backbone in this artificial in vitro system. More comparative studies are further required to explore extended repertoires of scaffold‑paralleled multi‑shRNAmiRs in more physiologically relevant models.
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Copy and paste a formatted citation
Spandidos Publications style
Du X, Cai Y, Xi W, Zhang R, Jia L, Yang A, Zhao J and Yan B: Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones. Mol Med Rep 17: 307-314, 2018.
APA
Du, X., Cai, Y., Xi, W., Zhang, R., Jia, L., Yang, A. ... Yan, B. (2018). Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones. Molecular Medicine Reports, 17, 307-314. https://doi.org/10.3892/mmr.2017.7854
MLA
Du, X., Cai, Y., Xi, W., Zhang, R., Jia, L., Yang, A., Zhao, J., Yan, B."Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones". Molecular Medicine Reports 17.1 (2018): 307-314.
Chicago
Du, X., Cai, Y., Xi, W., Zhang, R., Jia, L., Yang, A., Zhao, J., Yan, B."Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones". Molecular Medicine Reports 17, no. 1 (2018): 307-314. https://doi.org/10.3892/mmr.2017.7854
Copy and paste a formatted citation
x
Spandidos Publications style
Du X, Cai Y, Xi W, Zhang R, Jia L, Yang A, Zhao J and Yan B: Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones. Mol Med Rep 17: 307-314, 2018.
APA
Du, X., Cai, Y., Xi, W., Zhang, R., Jia, L., Yang, A. ... Yan, B. (2018). Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones. Molecular Medicine Reports, 17, 307-314. https://doi.org/10.3892/mmr.2017.7854
MLA
Du, X., Cai, Y., Xi, W., Zhang, R., Jia, L., Yang, A., Zhao, J., Yan, B."Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones". Molecular Medicine Reports 17.1 (2018): 307-314.
Chicago
Du, X., Cai, Y., Xi, W., Zhang, R., Jia, L., Yang, A., Zhao, J., Yan, B."Multi‑target inhibition by four tandem shRNAs embedded in homo‑ or hetero‑miRNA backbones". Molecular Medicine Reports 17, no. 1 (2018): 307-314. https://doi.org/10.3892/mmr.2017.7854
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