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Article Open Access

Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48

  • Authors:
    • Shuying Li
    • Zhanjun Liu
    • Ji Li
    • Aihua Liu
    • Lihua Zhu
    • Kui Yu
    • Ke Zhang
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, North China University of Science and Technology (Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases), Tangshan, Hebei 063000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 763-770
    |
    Published online on: November 6, 2017
       https://doi.org/10.3892/mmr.2017.7986
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Abstract

The present study aimed to explore the effects of a stabilizing ligand, Shield‑1, on the replication of recombinant varicella‑zoster virus (VZV) containing FK506 binding protein (FKPB) tags in essential open reading frames (ORF) 4 and 48. A specific galactokinase (galK) selection method was conducted, following the addition of galK labels to VZV ORF4 and 48, using a SW102 VZV bacterial artificial chromosome (BAC) system. Subsequently, recombinant VZV containing FKPB tags in ORF4 and 48 was constructed by counterselection and homologous recombination. Recombinant viral plasmids containing FKPB‑tagged VZV ORF4 and 48 were extracted and transfected into human acute retinal pigment epithelial ARPE‑19 cells. The results demonstrated that the FKPB‑tagged viral protein was rapidly degraded by proteases in recombinant virus‑infected ARPE‑19 cells. In addition, the recombinant VZVORF4‑FKBP‑ORF48‑FKBP virus could not grow if a synthetic ligand of FKBP, Shield1, was not added to the ARPE‑19 cell culture medium; however, the degradation of FKPB‑tagged viral protein was prevented if Shield1 was added to the ARPE‑19 cell culture medium, thereby allowing viral replication in ARPE‑19 cells. These results indicated that Shield1 may regulate replication of recombinant VZVORF4‑FKBP‑ORF48‑FKBP following transfection into human epithelial cells.
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View References

1 

Visalli MA, House BL, Selariu A, Zhu H and Visalli RJ: The varicella-zoster virus portal protein is essential for cleavage and packaging of viral DNA. J Virol. 88:7973–7986. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Khalil MI, Arvin A, Jones J and Ruyechan WT: A sequence within the varicella-zoster virus (VZV) OriS is a negative regulator of DNA replication and is bound by a protein complex containing the VZV ORF29 protein. J Virol. 85:12188–12200. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Berarducci B, Rajamani J, Zerboni L, Che X, Sommer M and Arvin AM: Functions of the unique N-terminal region of glycoprotein E in the pathogenesis of varicella-zoster virus infection. Proc Natl Acad Sci USA. 107:282–287. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zhang Z, Selariu A, Warden C, Huang G, Huang Y, Zaccheus O, Cheng T, Xia N and Zhu H: Genome-wide mutagenesis reveals that ORF7 is a novel VZV skin-tropic factor. PLoS Pathog. 6:e10009712010. View Article : Google Scholar : PubMed/NCBI

5 

Zhang Z, Huang Y and Zhu H: An efficient protocol for VZV BAC-based mutagenesis. Methods Mol Biol. 634:75–86. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Z, Huang Y and Zhu H: A highly efficient protocol of generating and analyzing VZV ORF deletion mutants based on a newly developed luciferase VZV BAC system. J Virol Methods. 148:197–204. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Zhang Z, Rowe J, Wang W, Sommer M, Arvin A, Moffat J and Zhu H: Genetic analysis of varicella-zoster virus ORF0 to ORF4 by use of a novel luciferase bacterial artificial chromosome system. J Virol. 81:9024–9033. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Takahashi M, Asano Y, Kamiya H, Baba K, Ozaki T, Otsuka T and Yamanishi K: Development of varicella vaccine. J Infect Dis. 197 Suppl 2:S41–S44. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Arvin AM: Varicella-zoster virus. Clin Microbiol Rev. 9:361–381. 1996.PubMed/NCBI

10 

Cohen JI: Varicella-zoster vaccine virus: Evolution in action. Proc Natl Acad Sci USA. 104:7–8. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Centers for Disease Control and Prevention (CDC): Decline in annual incidence of varicella-selected states, 1990–2001. MMWR Morb Mortal Wkly Rep. 52:884–885. 2003.PubMed/NCBI

12 

Agopian A, Lopez A, Wilson D, Peralta V, El Amin AN and Bialek S: Varicella hospitalizations in Los Angeles during the varicella vaccination era, 2003–2011: Are they preventable? Vaccine. 32:5353–5356. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Baxter R, Tran TN, Ray P, Lewis E, Fireman B, Black S, Shinefield HR, Coplan PM and Saddier P: Impact of vaccination on the epidemiology of varicella: 1995–2009. Pediatrics. 134:24–30. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Galil K, Lee B, Strine T, Carraher C, Baughman AL, Eaton M, Montero J and Seward J: Outbreak of varicella at a day-care center despite vaccination. N Engl J Med. 347:1909–1915. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Gan L, Wang M, Yang S, Gershon AA and Chen JJ: Transmission of varicella vaccine virus to a non-family member in China. Vaccine. 29:2015–2017. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Oxman MN, Levin MJ, Johnson GR, Schmader KE, Straus SE, Gelb LD, Arbeit RD, Simberkoff MS, Gershon AA, Davis LE, et al: A vaccine to prevent herpes zoster and postherpetic neuralgia in older adults. N Engl J Med. 352:2271–2284. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Das S, Ortiz DA, Gurczynski SJ, Khan F and Pellett PE: Identification of human cytomegalovirus genes important for biogenesis of the cytoplasmic virion assembly complex. J Virol. 88:9086–9099. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Maetzig T, Kuehle J, Schwarzer A, Turan S, Rothe M, Chaturvedi A, Morgan M, Ha TC, Heuser M, Hammerschmidt W, et al: All-in-One inducible lentiviral vector systems based on drug controlled FLP recombinase. Biomaterials. 35:4345–4356. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Perng YC, Qian Z, Fehr AR, Xuan B and Yu D: The human cytomegalovirus gene UL79 is required for the accumulation of late viral transcripts. J Virol. 85:4841–4852. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG and Wandless TJ: A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell. 126:995–1004. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Murphy E, Yu D, Grimwood J, Schmutz J, Dickson M, Jarvis MA, Hahn G, Nelson JA, Myers RM and Shenk TE: Coding potential of laboratory and clinical strains of human cytomegalovirus. Proc Natl Acad Sci USA. 100:14976–14981. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Warden C, Tang Q and Zhu H: Herpesvirus BACs: Past, present, and future. J Biomed Biotechnol. 2011:1245952011. View Article : Google Scholar : PubMed/NCBI

23 

Yu D, Silva MC and Shenk T: Functional map of human cytomegalovirus AD169 defined by global mutational analysis. Proc Natl Acad Sci USA. 100:12396–12401. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Bonger KM, Chen LC, Liu CW and Wandless TJ: Small molecule displacement of a cryptic degron causes conditional protein degradation. Nat Chem Biol. 7:531–537. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Banaszynski LA, Sellmyer MA, Contag CH, Wandless TJ and Thorne SH: Chemical control of protein stability and function in living mice. Nat Med. 14:1123–1127. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Sellmyer MA, Thorne SH, Banaszynski LA, Contag CH and Wandless TJ: A general method for conditional regulation of protein stability in living animals. Cold Spring Harb Protoc. 2009:pdb.prot51732009. View Article : Google Scholar : PubMed/NCBI

27 

Ma YF, Weiss LM and Huang H: A method for rapid regulation of protein expression in Trypanosoma cruzi. Int J Parasitol. 42:33–37. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li S, Liu Z, Li J, Liu A, Zhu L, Yu K and Zhang K: Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48. Mol Med Rep 17: 763-770, 2018.
APA
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., & Zhang, K. (2018). Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48. Molecular Medicine Reports, 17, 763-770. https://doi.org/10.3892/mmr.2017.7986
MLA
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., Zhang, K."Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48". Molecular Medicine Reports 17.1 (2018): 763-770.
Chicago
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., Zhang, K."Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48". Molecular Medicine Reports 17, no. 1 (2018): 763-770. https://doi.org/10.3892/mmr.2017.7986
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Liu Z, Li J, Liu A, Zhu L, Yu K and Zhang K: Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48. Mol Med Rep 17: 763-770, 2018.
APA
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., & Zhang, K. (2018). Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48. Molecular Medicine Reports, 17, 763-770. https://doi.org/10.3892/mmr.2017.7986
MLA
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., Zhang, K."Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48". Molecular Medicine Reports 17.1 (2018): 763-770.
Chicago
Li, S., Liu, Z., Li, J., Liu, A., Zhu, L., Yu, K., Zhang, K."Effects of Shield1 on the viral replication of varicella‑zoster virus containing FKBP‑tagged ORF4 and 48". Molecular Medicine Reports 17, no. 1 (2018): 763-770. https://doi.org/10.3892/mmr.2017.7986
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