Anti‑inflammatory effect of Migri‑Heal® in an in vitro inflammatory model of primary mixed glial cells

Hassani,Mahmoud; Sabouni,Farzaneh; Ansari,Mohammad; Emamgholipour,Solaleh; Fallah,Mohammad‑Sadegh; Abbasi,Shah‑Sanam; Ansari Majd,Saeed

doi:10.3892/mmr.2017.8027

Anti‑inflammatory effect of Migri‑Heal® in an in vitro inflammatory model of primary mixed glial cells

Authors:
- Mahmoud Hassani
- Farzaneh Sabouni
- Mohammad Ansari
- Solaleh Emamgholipour
- Mohammad‑Sadegh Fallah
- Shah‑Sanam Abbasi
- Saeed Ansari Majd
View Affiliations

Affiliations: Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran, Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran 1497716316, Iran, Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran 1417613151, Iran, Kawsar Human Genetics Research Center, Tehran 1595645513, Iran
Published online on: November 13, 2017 https://doi.org/10.3892/mmr.2017.8027
Pages: 1901-1906

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Migri‑Heal®, is a novel herbal remedy that was introduced for the treatment of migraine headaches. Previous studies revealed that this drug may reduce nitric oxide (NO) in an in vitro inflammatory model. The aim of the present study was to investigate the anti‑inflammatory effect of Migri‑Heal® on primary mix glial cells stimulated with LPS. In the current study, neonatal rat primary mix glial cells were isolated from the mixed glial cultures via shaking, and cultured in Dulbecco's' modified Eagle's medium supplemented with 10% fetal bovine serum. Following pretreatment with Migri‑Heal® (25, 75, 100, 150, 200 and 300 µg/ml) and cells were treated with LPS (10 µg/ml) for 1 h, and incubated for 48 h. The present study determined that 150 µg/ml Migri‑Heal® signiﬁcantly reduced the production of NO in rat mix glial cells stimulated with 10 µg/ml LPS. Migri‑Heal® also suppressed mRNA expression level of LPS‑induced inducible nitric oxide synthase and tumor necrosis factor α, which was accompanied by inhibition of the transcription factor nuclear factor‑κB. Additionally, MTT assay determined that Migri‑Heal® was not cytotoxic, suggesting that the anti‑inflammatory effects of Migri‑Heal® observed were not due to cell death. In conclusion, the findings of the present study demonstrated that Migri‑Heal® may be useful as a potential anti‑inflammatory agent in inflammatory diseases. However, additional studies are required to confirm these findings.

View Figures

View References

1	Allan SM and Rothwell NJ: Cytokines and acute neurodegeneration. Nat Rev Neurosci. 2:734–744. 2001. View Article : Google Scholar
2	Nguyen MD, Julien JP and Rivest S: Innate immunity: The missing link in neuroprotection and neurodegeneration? Nat Rev Neurosci. 3:216–227. 2002. View Article : Google Scholar
3	Shibakawa YS, Sasaki Y, Goshima Y, Echigo N, Kamiya Y, Kurahashi K, Yamada Y and Andoh T: Effects of ketamine and propofol on inflammatory responses of primary glial cell cultures stimulated with lipopolysaccharide. Br J Anaesth. 95:803–810. 2005. View Article : Google Scholar
4	Polazzi E and Monti B: Microglia and neuroprotection: From in vitro studies to therapeutic applications. Prog Neurobiol. 92:293–315. 2010. View Article : Google Scholar
5	Hirsch EC, Hunot S, Damier P and Faucheux B: Glial cells and inflammation in Parkinson's disease: A role in neurodegeneration? Ann Neurol. 44 3 Suppl 1:S115–S120. 1998. View Article : Google Scholar
6	Miller DW, Cookson MR and Dickson DW: Glial cell inclusions and the pathogenesis of neurodegenerative diseases. Neuron Glia Biol. 1:13–21. 2004. View Article : Google Scholar :
7	Chang RC, Hudson P, Wilson B, Haddon L and Hong JS: Influence of neurons on lipopolysaccharide-stimulated production of nitric oxide and tumor necrosis factor-alpha by cultured glia. Brain Res. 853:236–244. 2000. View Article : Google Scholar
8	Tikka T, Fiebich BL, Goldsteins G, Keinanen R and Koistinaho J: Minocycline, a tetracycline derivative, is neuroprotective against excitotoxicity by inhibiting activation and proliferation of microglia. J Neurosci. 21:2580–2588. 2001.
9	Wu DC, Jackson-Lewis V, Vila M, Tieu K, Teismann P, Vadseth C, Choi DK, Ischiropoulos H and Przedborski S: Blockade of microglial activation is neuroprotective in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson disease. J Neurosci. 22:1763–1771. 2002.
10	Ansari M, Rafiee K, Emamgholipour S and Fallah MS: Migraine: Molecular basis and herbal medicine. Chen KS: Advanced Topics in Neurological Disorders: InTech. 185–214. 2012.
11	Rafiee K, Ansaria M, Mahdian R, Paknejad M, Fallah MS and Azizi M: In vitro effects of a herbal remedy for migraine treatment, migriHeal^®, on basal and LPS-induced nitric oxide. J Basic Appl Sci Res. 3:206–211. 2013.
12	Obuchowicz E, Kowalski J, Labuzek K, Krysiak R, Pendzich J and Herman ZS: Amitriptyline and nortriptyline inhibit interleukin-1 release by rat mixed glial and microglial cell cultures. Int J Neuropsychopharmacol. 9:27–35. 2006. View Article : Google Scholar
13	Zanassi P, Paolillo M, Montecucco A, Avvedimento EV and Schinelli S: Pharmacological and molecular evidence for dopamine D(1) receptor expression by striatal astrocytes in culture. J Neurosci Res. 58:544–552. 1999. View Article : Google Scholar
14	Cao L, Fei L, Chang TT and DeLeo JA: Induction of interleukin-1beta by interleukin-4 in lipopolysaccharide-treated mixed glial cultures: Microglial-dependent effects. J Neurochem. 102:408–419. 2007. View Article : Google Scholar
15	Amiraslani B, Sabouni F, Abbasi S, Nazem H and Sabet M: Recognition of betaine as an inhibitor of lipopolysaccharide-induced nitric oxide production in activated microglial cells. Iran Biomed J. 16:84–89. 2012.
16	Javadian S, Sabouni F and Haghbeen K: O riganum V ulgare L.: Extracts versus thymol: An anti-inflammatory study on activated microglial and mixed glial cells. J Food Biochem. 40:100–108. 2016. View Article : Google Scholar
17	Habashi Abbasi S, Sabouni F, Moghimi A and Majd Ansari S: Modulation of lipopolysaccharide stimulated nuclear factor kappa B mediated iNOS/NO production by bromelain in rat primary microglial cells. Iran Biomed J. 20:33–40. 2016.
18	Mossman BT, Jean L and Landesman JM: Studies using lectins to determine mineral interactions with cellular membranes. Environ Health Perspect. 51:23–25. 1983. View Article : Google Scholar :
19	Smale ST: Selective transcription in response to an inflammatory stimulus. Cell. 140:833–844. 2010. View Article : Google Scholar :
20	Doherty GH: Nitric oxide in neurodegeneration: Potential benefits of non-steroidal anti-inflammatories. Neurosci Bull. 27:366–382. 2011. View Article : Google Scholar :
21	Craft JM, Watterson DM, Frautschy SA and Van Eldik LJ: Aminopyridazines inhibit beta-amyloid-induced glial activation and neuronal damage in vivo. Neurobiol Aging. 25:1283–1292. 2004. View Article : Google Scholar
22	Bogdan C: Nitric oxide and the immune response. Nat Immunol. 2:907–916. 2001. View Article : Google Scholar
23	MacMicking J, Xie QW and Nathan C: Nitric oxide and macrophage function. Annu Rev Immunol. 15:323–350. 1997. View Article : Google Scholar
24	Sarchielli P, Floridi A, Mancini ML, Rossi C, Coppola F, Baldi A, Pini LA and Calabresi P: NF-kappaB activity and iNOS expression in monocytes from internal jugular blood of migraine without aura patients during attacks. Cephalalgia. 26:1071–1079. 2006. View Article : Google Scholar
25	Stirparo G, Zicari A, Favilla M, Lipari M and Martelletti P: Linked activation of nitric oxide synthase and cyclooxygenase in peripheral monocytes of asymptomatic migraine without aura patients. Cephalalgia. 20:100–106. 2000. View Article : Google Scholar
26	Hassani M, Sabouni F, Emamgholipour S, Rafiee MH, Fallah MS, Abbasi SS and Ansari M: The effect of Migri-Heal^® on nitric oxide production in an in vitro inflammatory model of primary microglial cells. Arch Med Lab Sci. 2:54–61. 2016.
27	Ansari M, Rafiee Kh, Yasa N, Vardasbi S, Naimi SM and Nowrouzi A: Measurement of melatonin in alcoholic and hot water extracts of Tanacetum parthenium, Tripleurospermum disciforme and Viola odorata. Daru. 18:173–178. 2010.
28	Gilad E, Wong HR, Zingarelli B, Virág L, O'Connor M, Salzman AL and Szabó C: Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: Role of inhibition of NFkappaB activation. FASEB J. 12:685–693. 1998.
29	Peres MF, Masruha MR, Zukerman E, Moreira-Filho CA and Cavalheiro EA: Potential therapeutic use of melatonin in migraine and other headache disorders. Expert Opin Investig Drugs. 15:367–375. 2006. View Article : Google Scholar
30	Fooladsaz K, Ansari M and Rasaie MJ: Evaluation and comparison of serum melatonin determination in normal individuals and migraine patients. Tehran Univ Med J. 62:37–43. 2004.
31	Kavoosi G, da Silva Teixeira JA and Saharkhiz MJ: Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide-stimulated macrophages. J Pharm Pharmacol. 64:1491–1500. 2012. View Article : Google Scholar
32	Alemi M, Sabouni F, Sanjarian F, Haghbeen K and Ansari S: Anti-inflammatory effect of seeds and callus of Nigella sativa L. extracts on mix glial cells with regard to their thymoquinone content. AAPS Pharm Sci Tech. 14:160–167. 2013. View Article : Google Scholar
33	Saijo K, Winner B, Carson CT, Collier JG, Boyer L, Rosenfeld MG, Gage FH and Glass CK: A Nurr1/CoREST pathway in microglia and astrocytes protects dopaminergic neurons from inflammation-induced death. Cell. 137:47–59. 2009. View Article : Google Scholar :