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Article

iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability

  • Authors:
    • Xiangrong Liu
    • Jun Kang
    • Si Sun
    • Yumin Luo
    • Xunming Ji
    • Xianwei Zeng
    • Shangfeng Zhao
  • View Affiliations / Copyright

    Affiliations: Cerebrovascular Diseases Research Institute, Xuanwu Hospital of Capital Medical University, Beijing 100053, P.R. China, Department of Neurosurgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Department of Neurosurgery, The Affiliated Hospital of Weifang Medical College, Weifang, Shandong 261031, P.R. China
  • Pages: 1970-1978
    |
    Published online on: November 15, 2017
       https://doi.org/10.3892/mmr.2017.8097
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Abstract

MicroRNAs (miRNAs) regulate biogenesis and disease development by targeting numerous mRNAs. miRNA (miR)‑124 and its direct target, inhibitor of apoptosis‑stimulating protein of p53 (iASPP), may be involved in tumor development and progression. The aim of the present study was to explore the role of miR‑124‑targeted iASPP in glioma. The results demonstrated that miR‑124 was aberrantly expressed in astrocytic glioma tissue and in the human glioblastoma cell lines U87 and U251. The expression of miR‑124 was lower in astrocytic gliomas compared with normal brain (NB) tissues, with a more reduced expression in higher‑grade tumors. In addition, several miR‑124 loci (including miR‑124‑1, miR‑124‑2 and miR‑124‑3) were revealed to be more highly methylated in U87 cells compared with methylation levels in U251 cells and NB cells. Furthermore, the expression of iASPP was higher in high‑grade astrocytic gliomas compared with low‑grade astrocytic gliomas. miR‑124 overexpression effectively inhibited U87 and U251 cell migration. In addition, miR‑124 regulated cell viability and arrested the cell cycle at the G0/G1 phase in these two cell lines. miR‑124 also reduced the expression levels of the cell cycle related genes iASPP, cyclin‑dependent kinase (CDK)4, CDK6 and cyclin D1. Results from the present study indicated that expression of the miR‑124 target gene iASPP may contribute to glioma development and progression.
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View References

1 

Goodenberger ML and Jenkins RB: Genetics of adult glioma. Cancer Genet. 205:613–621. 2012. View Article : Google Scholar

2 

Dunbar E and Yachnis AT: Glioma diagnosis: Immunohistochemistry and beyond. Adv Anat Pathol. 17:187–201. 2010. View Article : Google Scholar

3 

Shenoy A and Blelloch RH: Regulation of microRNA function in somatic stem cell proliferation and differentiation. Nat Rev Mol Cell Biol. 15:565–576. 2014. View Article : Google Scholar :

4 

Lin S and Gregory RI: MicroRNA biogenesis pathways in cancer. Nat Rev Cancer. 15:321–333. 2015. View Article : Google Scholar :

5 

Garofalo M and Croce CM: Role of microRNAs in maintaining cancer stem cells. Adv Drug Deliv Rev. 81:53–61. 2015. View Article : Google Scholar

6 

Jiang C, Chen X, Alattar M, Wei J and Liu H: MicroRNAs in tumorigenesis, metastasis, diagnosis and prognosis of gastric cancer. Cancer Gene Ther. 22:291–301. 2015. View Article : Google Scholar

7 

Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W and Tuschl T: Identification of tissue-specific microRNAs from mouse. Curr Biol. 12:735–739. 2002. View Article : Google Scholar

8 

Kloosterman WP, Wienholds E, de Bruijn E, Kauppinen S and Plasterk RH: In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat Methods. 3:27–29. 2006. View Article : Google Scholar

9 

Cao X, Pfaff SL and Gage FH: A functional study of miR-124 in the developing neural tube. Genes Dev. 21:531–536. 2007. View Article : Google Scholar :

10 

Cao Q, Li YY, He WF, Zhang ZZ, Zhou Q, Liu X, Shen Y and Huang TT: Interplay between microRNAs and the STAT3 signaling pathway in human cancers. Physiol Genomics. 45:1206–1214. 2013. View Article : Google Scholar

11 

Chen T, Wang XY, Li C and Xu SJ: Downregulation of microRNA-124 predicts poor prognosis in glioma patients. Neurol Sci. 36:131–135. 2015. View Article : Google Scholar

12 

Shi Z, Chen Q, Li C, Wang L, Qian X, Jiang C, Liu X, Wang X, Li H, Kang C, et al: MiR-124 governs glioma growth and angiogenesis and enhances chemosensitivity by targeting R-Ras and N-Ras. Neuro Oncol. 16:1341–1353. 2014. View Article : Google Scholar :

13 

An L, Liu Y, Wu A and Guan Y: microRNA-124 inhibits migration and invasion by down-regulating ROCK1 in glioma. PLoS One. 8:e694782013. View Article : Google Scholar :

14 

Shkumatava A, Stark A, Sive H and Bartel DP: Coherent but overlapping expression of microRNAs and their targets during vertebrate development. Genes Dev. 23:466–481. 2009. View Article : Google Scholar :

15 

Clark AM, Goldstein LD, Tevlin M, Tavaré S, Shaham S and Miska EA: The microRNA miR-124 controls gene expression in the sensory nervous system of Caenorhabditis elegans. Nucleic Acids Res. 38:3780–3793. 2010. View Article : Google Scholar :

16 

Chi SW, Zang JB, Mele A and Darnell RB: Argonaute HITS-CLIP decodes microRNA-mRNA interaction maps. Nature. 460:479–486. 2009.

17 

Pierson J, Hostager B, Fan R and Vibhakar R: Regulation of cyclin dependent kinase 6 by microRNA 124 in medulloblastoma. J Neurooncol. 90:1–7. 2008. View Article : Google Scholar

18 

Liu X, Li F, Zhao S, Luo Y, Kang J, Zhao H, Yan F, Li S and Ji X: MicroRNA-124-mediated regulation of inhibitory member of apoptosis-stimulating protein of p53 family in experimental stroke. Stroke. 44:1973–1980. 2013. View Article : Google Scholar

19 

Lu SH, Jiang XJ, Xiao GL, Liu DY and Yuan XR: miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin. Oncol Rep. 32:2104–2110. 2014. View Article : Google Scholar

20 

Liu Z, Zhang X, Huang D, Liu Y, Zhang X, Liu L, Li G, Dai Y, Tan H, Xiao J and Tian Y: Elevated expression of iASPP in head and neck squamous cell carcinoma and its clinical significance. Med Oncol. 29:3381–3388. 2012. View Article : Google Scholar

21 

Wang LL, Xu Z, Peng Y, Li LC and Wu XL: Downregulation of inhibitor of apoptosis-stimulating protein of p53 inhibits proliferation and promotes apoptosis of gastric cancer cells. Mol Med Rep. 12:1653–1658. 2015. View Article : Google Scholar :

22 

Zhao WH, Wu SQ and Zhang YD: Downregulation of miR-124 promotes the growth and invasiveness of glioblastoma cells involving upregulation of PPP1R13L. Int J Mol Med. 32:101–107. 2013. View Article : Google Scholar

23 

Zhang Y, Zheng L, Huang J, Gao F, Lin X, He L, Li D, Li Z, Ding Y and Chen L: MiR-124 Radiosensitizes human colorectal cancer cells by targeting PRRX1. PLoS One. 9:e939172014. View Article : Google Scholar :

24 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar

25 

Wang D, Zhang H, Li M, Frid MG, Flockton AR, McKeon BA, Yeager ME, Fini MA, Morrell NW, Pullamsetti SS, et al: MicroRNA-124 controls the proliferative, migratory and inflammatory phenotype of pulmonary vascular fibroblasts. Circ Res. 114:67–78. 2014. View Article : Google Scholar

26 

Liu X, Wang L, Zhao S, Ji X, Luo Y and Ling F: β-catenin overexpression in malignant glioma and its role in proliferation and apoptosis in glioblastoma cells. Med Oncol. 28:608–614. 2011. View Article : Google Scholar

27 

Zhou X, Ruan J, Wang G and Zhang W: Characterization and identification of microRNA core promoters in four model species. PLoS Comput Biol. 3:e372007. View Article : Google Scholar :

28 

Sato F, Tsuchiya S, Meltzer SJ and Shimizu K: MicroRNAs and epigenetics. FEBS J. 278:1598–1609. 2011. View Article : Google Scholar

29 

Papagiannakopoulos T and Kosik KS: MicroRNAs: Regulators of oncogenesis and stemness. BMC Med. 6:152008. View Article : Google Scholar :

30 

Toiyama Y, Okugawa Y and Goel A: DNA methylation and microRNA biomarkers for noninvasive detection of gastric and colorectal cancer. Biochem Biophys Res Commun. 455:43–57. 2014. View Article : Google Scholar :

31 

Silber J, Lim DA, Petritsch C, Persson AI, Maunakea AK, Yu M, Vandenberg SR, Ginzinger DG, James CD, Costello JF, et al: miR-124 and miR-137 inhibit proliferation of glioblastoma multiforme cells and induce differentiation of brain tumor stem cells. BMC Med. 6:142008. View Article : Google Scholar :

32 

Li G, Wang R, Gao J, Deng K, Wei J and Wei Y: RNA interference-mediated silencing of iASPP induces cell proliferation inhibition and G0/G1 cell cycle arrest in U251 human glioblastoma cells. Mol Cell Biochem. 350:193–200. 2011. View Article : Google Scholar

33 

Cai Y, Qiu S, Gao X, Gu SZ and Liu ZJ: iASPP inhibits p53-independent apoptosis by inhibiting transcriptional activity of p63/p73 on promoters of proapoptotic genes. Apoptosis. 17:777–783. 2012. View Article : Google Scholar

34 

Chikh A, Sanzà P, Raimondi C, Akinduro O, Warnes G, Chiorino G, Byrne C, Harwood CA and Bergamaschi D: iASPP is a novel autophagy inhibitor in keratinocytes. J Cell Sci. 127:3079–3093. 2014. View Article : Google Scholar

35 

Deng Q, Sheng L, Su D, Zhang L, Liu P, Lu K and Ma S: Genetic polymorphisms in ATM, ERCC1, APE1 and iASPP genes and lung cancer risk in a population of southeast China. Med Oncol. 28:667–672. 2011. View Article : Google Scholar

36 

Zhang X, Wang M, Zhou C, Chen S and Wang J: The expression of iASPP in acute leukemias. Leuk Res. 29:179–183. 2005. View Article : Google Scholar

37 

Lin BL, Xie DY, Xie SB, Xie JQ, Zhang XH, Zhang YF and Gao ZL: Down-regulation of iASPP in human hepatocellular carcinoma cells inhibits cell proliferation and tumor growth. Neoplasma. 58:205–210. 2011. View Article : Google Scholar

38 

Deng X, Ma L, Wu M, Zhang G, Jin C, Guo Y and Liu R: miR-124 radiosensitizes human glioma cells by targeting CDK4. J Neurooncol. 114:263–274. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Kang J, Sun S, Luo Y, Ji X, Zeng X and Zhao S: iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability. Mol Med Rep 17: 1970-1978, 2018.
APA
Liu, X., Kang, J., Sun, S., Luo, Y., Ji, X., Zeng, X., & Zhao, S. (2018). iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability. Molecular Medicine Reports, 17, 1970-1978. https://doi.org/10.3892/mmr.2017.8097
MLA
Liu, X., Kang, J., Sun, S., Luo, Y., Ji, X., Zeng, X., Zhao, S."iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability". Molecular Medicine Reports 17.1 (2018): 1970-1978.
Chicago
Liu, X., Kang, J., Sun, S., Luo, Y., Ji, X., Zeng, X., Zhao, S."iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability". Molecular Medicine Reports 17, no. 1 (2018): 1970-1978. https://doi.org/10.3892/mmr.2017.8097
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Kang J, Sun S, Luo Y, Ji X, Zeng X and Zhao S: iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability. Mol Med Rep 17: 1970-1978, 2018.
APA
Liu, X., Kang, J., Sun, S., Luo, Y., Ji, X., Zeng, X., & Zhao, S. (2018). iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability. Molecular Medicine Reports, 17, 1970-1978. https://doi.org/10.3892/mmr.2017.8097
MLA
Liu, X., Kang, J., Sun, S., Luo, Y., Ji, X., Zeng, X., Zhao, S."iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability". Molecular Medicine Reports 17.1 (2018): 1970-1978.
Chicago
Liu, X., Kang, J., Sun, S., Luo, Y., Ji, X., Zeng, X., Zhao, S."iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates malignant glioma cell migration and viability". Molecular Medicine Reports 17, no. 1 (2018): 1970-1978. https://doi.org/10.3892/mmr.2017.8097
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