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Article

MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages

  • Authors:
    • Qingyun Xie
    • Meng Wei
    • Bo Zhang
    • Xia Kang
    • Da Liu
    • Wei Zheng
    • Xianming Pan
    • Yi Quan
    • Dongfa Liao
    • Jun Shen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Chengdu Military General Hospital, Jinniu, Chengdu, Sichuan 610083, P.R. China, Department of Nephrology and Rheumatology, Chengdu Military General Hospital, Jinniu, Chengdu, Sichuan 610083, P.R. China
  • Pages: 3318-3327
    |
    Published online on: December 8, 2017
       https://doi.org/10.3892/mmr.2017.8224
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Abstract

The nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3) inflammasome/interleukin (IL)-1β axis serves an essential role in regulating the development of rheumatoid arthritis (RA). The dysregulation of cellular metabolism, such as mitochondrial dysfunction, results in the activation of the NLRP3 inflammasome. microRNA (miR)‑33 has previously been identified to be a regulator of lipid metabolism and mitochondrial function. However, whether miR‑33 regulates the NLRP3 inflammasome/IL‑1β axis remains unknown. In the present study, it was observed that an miR‑33 mimic or anti‑miR‑33 markedly stimulated or inhibited, respectively, IL‑1β protein expression levels in mouse peritoneal macrophages. Mechanistically, miR‑33 upregulated the expression of NLRP3 mRNA and protein as well as caspase‑1 activity in primary macrophages. In addition, the results demonstrated that miR‑33 impaired mitochondrial oxygen consumption rates, resulting in the accumulation of cellular reactive oxygen species, which stimulated NLRP3 expression, caspase‑1 activity and IL‑1β secretion. The results of the present study demonstrated that miR‑33 levels and NLRP3 inflammasome activity were increased in peripheral blood monocytes from patients with RA patients compared with healthy donors. In conclusion, the present study identified miR‑33 to be a positive regulator of the NLRP3 inflammasome in macrophages. The miR‑33/NLRP3 inflammasome pathway may therefore be involved in RA development.
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View References

1 

Strowig T, Henao-Mejia J, Elinav E and Flavell R: Inflammasomes in health and disease. Nature. 481:278–286. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Eisenbarth SC, Colegio OR, O'Connor W, Sutterwala FS and Flavell RA: Crucial role for the Nalp3 inflammasome in the immunostimulatory properties of aluminium adjuvants. Nature. 453:1122–1126. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Schroder K, Zhou R and Tschopp J: The NLRP3 inflammasome: A sensor for metabolic danger? Science. 327:296–300. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zhou R, Yazdi AS, Menu P and Tschopp J: A role for mitochondria in NLRP3 inflammasome activation. Nature. 469:221–225. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Miao H, Ou J, Ma Y, Guo F, Yang Z, Wiggins M, Liu C, Song W, Han X, Wang M, et al: Macrophage CGI-58 deficiency activates ROS-inflammasome pathway to promote insulin resistance in mice. Cell Rep. 7:223–235. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Siebert S, Tsoukas A, Robertson J and McInnes I: Cytokines as therapeutic targets in rheumatoid arthritis and other inflammatory diseases. Pharmacol Rev. 67:280–309. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Cunnane G, Madigan A, Murphy E, FitzGerald O and Bresnihan B: The effects of treatment with interleukin-1 receptor antagonist on the inflamed synovial membrane in rheumatoid arthritis. Rheumatology (Oxford). 40:62–69. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Ma Z, Wang B, Wang M, Sun X, Tang Y, Li M, Li F and Li X: TL1A increased IL-6 production on fibroblast-like synoviocytes by preferentially activating TNF receptor 2 in rheumatoid arthritis. Cytokine. 83:92–98. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Fontalba A, Martinez-Taboada V, Gutierrez O, Pipaon C, Benito N, Balsa A, Blanco R and Fernandez-Luna JL: Deficiency of the NF-kappaB inhibitor caspase activating and recruitment domain 8 in patients with rheumatoid arthritis is associated with disease severity. J Immunol. 179:4867–4873. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Kastbom A, Verma D, Eriksson P, Skogh T, Wingren G and Söderkvist P: Genetic variation in proteins of the cryopyrin inflammasome influences susceptibility and severity of rheumatoid arthritis (the Swedish TIRA project). Rheumatology (Oxford). 47:415–417. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Ben Hamad M, Cornelis F, Marzouk S, Chabchoub G, Bahloul Z, Rebai A, Fakhfakh F, Ayadi H, Petit-Teixeira E and Maalej A: Association study of CARD8 (p.C10X) and NLRP3 (p.Q705K) variants with rheumatoid arthritis in French and Tunisian populations. Int J Immunogenet. 39:131–136. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Walle LV, Van Opdenbosch N, Jacques P, Fossoul A, Verheugen E, Vogel P, Beyaert R, Elewaut D, Kanneganti TD, van Loo G and Lamkanfi M: Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis. Nature. 512:69–73. 2014.PubMed/NCBI

13 

Wen H, Gris D, Lei Y, Jha S, Zhang L, Huang MT, Brickey WJ and Ting JP: Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling. Nat Immunol. 12:408–415. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Heneka MT, Kummer MP, Stutz A, Delekate A, Schwartz S, Vieira-Saecker A, Griep A, Axt D, Remus A, Tzeng TC, et al: NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. Nature. 493:674–678. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Wree A, Eguchi A, McGeough MD, Pena CA, Johnson CD, Canbay A, Hoffman HM and Feldstein AE: NLRP3 inflammasome activation results in hepatocyte pyroptosis, liver inflammation, and fibrosis in mice. Hepatology. 59:898–910. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Shalgi R, Lieber D, Oren M and Pilpel Y: Global and local architecture of the mammalian microRNA-transcription factor regulatory network. PLoS Comput Biol. 3:e1312007. View Article : Google Scholar : PubMed/NCBI

17 

Ryazansky SS, Gvozdev VA and Berezikov E: Evidence for post-transcriptional regulation of clustered microRNAs in Drosophila. BMC Genomics. 12:3712011. View Article : Google Scholar : PubMed/NCBI

18 

Krützfeldt J and Stoffel M: MicroRNAs: A new class of regulatory genes affecting metabolism. Cell Metab. 4:9–12. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Rayner KJ, Suárez Y, Dávalos A, Parathath S, Fitzgerald ML, Tamehiro N, Fisher EA, Moore KJ and Fernández-Hernando C: MiR-33 contributes to the regulation of cholesterol homeostasis. Science. 328:1570–1573. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Esau CC, Hussain FN, McDaniel AL, Marshall SM, van Gils JM, Ray TD, Sheedy FJ, Goedeke L, Liu X, Khatsenko OG, et al: Inhibition of miR-33a/b in non-human primates raises plasma HDL and lowers VLDL triglycerides. Nature. 478:404–407. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Karunakaran D, Thrush AB, Nguyen MA, Richards L, Geoffrion M, Singaravelu R, Ramphos E, Shangari P, Ouimet M, Pezacki JP, et al: Macrophage mitochondrial energy status regulates cholesterol efflux and is enhanced by Anti-miR33 in atherosclerosis. Circ Res. 117:266–278. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Ouimet M, Ediriweera HN, Gundra UM, Sheedy FJ, Ramkhelawon B, Hutchison SB, Rinehold K, van Solingen C, Fullerton MD, Cecchini K, et al: MicroRNA-33-dependent regulation of macrophage metabolism directs immune cell polarization in atherosclerosis. J Clin Invest. 125:4334–4348. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Eklund KK, Leirisalo-Repo M, Ranta P, Mäki T, Kautiainen H, Hannonen P, Korpela M, Hakala M, Järvinen P and Möttönen T: Serum IL-1beta levels are associated with the presence of erosions in recent onset rheumatoid arthritis. Clin Exp Rheumatol. 25:684–689. 2007.PubMed/NCBI

25 

Ruscitti P, Cipriani P, Carubbi F, Liakouli V, Zazzeroni F, Di Benedetto P, Berardicurti O, Alesse E and Giacomelli R: The role of IL-1β in the bone loss during rheumatic diseases. Mediators Inflamm. 2015:7823822015. View Article : Google Scholar : PubMed/NCBI

26 

Spiegelman BM: Transcriptional control of mitochondrial energy metabolism through the PGC1 coactivators. Novartis Found Symp. 287:60–63. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Haemmerle G, Moustafa T, Woelkart G, Büttner S, Schmidt A, van de Weijer T, Hesselink M, Jaeger D, Kienesberger PC, Zierler K, et al: ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-α and PGC-1. Nat Med. 17:1076–1085. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Chen FY, Zhou J, Guo N, Ma WG, Huang X, Wang H and Yuan ZY: Curcumin retunes cholesterol transport homeostasis and inflammation response in M1 macrophage to prevent atherosclerosis. Biochem Biophys Res Commun. 467:872–878. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Chan KL, Pillon NJ, Sivaloganathan DM, Costford SR, Liu Z, Théret M, Chazaud B and Klip A: Palmitoleate reverses high fat-induced proinflammatory macrophage polarization via AMP-activated protein kinase (AMPK). J Biol Chem. 290:16979–16988. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Ho PC, Chang KC, Chuang YS and Wei LN: Cholesterol regulation of receptor-interacting protein 140 via microRNA-33 in inflammatory cytokine production. FASEB J. 25:1758–1766. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Choulaki C, Papadaki G, Repa A, Kampouraki E, Kambas K, Ritis K, Bertsias G, Boumpas DT and Sidiropoulos P: Enhanced activity of NLRP3 inflammasome in peripheral blood cells of patients with active rheumatoid arthritis. Arthritis Res Ther. 17:2572015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xie Q, Wei M, Zhang B, Kang X, Liu D, Zheng W, Pan X, Quan Y, Liao D, Shen J, Shen J, et al: MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages. Mol Med Rep 17: 3318-3327, 2018.
APA
Xie, Q., Wei, M., Zhang, B., Kang, X., Liu, D., Zheng, W. ... Shen, J. (2018). MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages. Molecular Medicine Reports, 17, 3318-3327. https://doi.org/10.3892/mmr.2017.8224
MLA
Xie, Q., Wei, M., Zhang, B., Kang, X., Liu, D., Zheng, W., Pan, X., Quan, Y., Liao, D., Shen, J."MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages". Molecular Medicine Reports 17.2 (2018): 3318-3327.
Chicago
Xie, Q., Wei, M., Zhang, B., Kang, X., Liu, D., Zheng, W., Pan, X., Quan, Y., Liao, D., Shen, J."MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages". Molecular Medicine Reports 17, no. 2 (2018): 3318-3327. https://doi.org/10.3892/mmr.2017.8224
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Q, Wei M, Zhang B, Kang X, Liu D, Zheng W, Pan X, Quan Y, Liao D, Shen J, Shen J, et al: MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages. Mol Med Rep 17: 3318-3327, 2018.
APA
Xie, Q., Wei, M., Zhang, B., Kang, X., Liu, D., Zheng, W. ... Shen, J. (2018). MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages. Molecular Medicine Reports, 17, 3318-3327. https://doi.org/10.3892/mmr.2017.8224
MLA
Xie, Q., Wei, M., Zhang, B., Kang, X., Liu, D., Zheng, W., Pan, X., Quan, Y., Liao, D., Shen, J."MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages". Molecular Medicine Reports 17.2 (2018): 3318-3327.
Chicago
Xie, Q., Wei, M., Zhang, B., Kang, X., Liu, D., Zheng, W., Pan, X., Quan, Y., Liao, D., Shen, J."MicroRNA‑33 regulates the NLRP3 inflammasome signaling pathway in macrophages". Molecular Medicine Reports 17, no. 2 (2018): 3318-3327. https://doi.org/10.3892/mmr.2017.8224
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