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Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells

  • Authors:
    • Zongwen Liu
    • Ting Chai
    • Yan Zhang
    • Alan Chu
    • Bing Liang
    • Xing Guo
    • Zhenjiang Guo
    • Rui Song
    • Ge Hou
    • Jinjin Yuan
    • Yaohe Liu
    • Yuhao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Pharmacology, School of Basic Medical of Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Department of Obstetrics and Gynecology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2817-2826
    |
    Published online on: December 8, 2017
       https://doi.org/10.3892/mmr.2017.8228
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Abstract

The present study aimed to detect the mutation characteristics of mitochondrial DNA (mtDNA) in Eca109 of Ec9706 cells, and to investigate their association with the nuclear genome (nDNA), thus providing a basis for gene targeting therapies for esophageal squamous cell carcinoma (ESCC). In vitro‑cultured Ec9706 and Eca109 cells were analyzed the changes of single‑nucleotide polymorphisms (SNPs), insertions/deletions (INDELs), copy number varia­tion, and structure variation (SV) of their genome by high‑throughput sequencing. The loci with SV on chromosome 1‑12 of the two ESCC cell lines were ≥5% of the mtDNA, but SV on chromosome 13‑22, X and Y was ≤3%; >40% of loci exhibited gain or loss; intergenic loci with INDEL changes and SNP features accounted for the majority of mutations. The affected genes encoded proteins including nDNA‑encoding intra‑mitochondrial‑transporting proteins, ATP energy generation‑associated proteins and mitochondrial electron respiratory chain proteins, and these proteins were all nucleus‑encoded mitochondrial proteins. The transcription, duplication, and translation of the abnormally expressed mtDNA in Ec9706 and Eca109 cells were closely associated with disorders of nuclear DNA products.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Chai T, Zhang Y, Chu A, Liang B, Guo X, Guo Z, Song R, Hou G, Yuan J, Yuan J, et al: Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells. Mol Med Rep 17: 2817-2826, 2018.
APA
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X. ... Zhang, Y. (2018). Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells. Molecular Medicine Reports, 17, 2817-2826. https://doi.org/10.3892/mmr.2017.8228
MLA
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X., Guo, Z., Song, R., Hou, G., Yuan, J., Liu, Y., Zhang, Y."Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells". Molecular Medicine Reports 17.2 (2018): 2817-2826.
Chicago
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X., Guo, Z., Song, R., Hou, G., Yuan, J., Liu, Y., Zhang, Y."Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells". Molecular Medicine Reports 17, no. 2 (2018): 2817-2826. https://doi.org/10.3892/mmr.2017.8228
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Chai T, Zhang Y, Chu A, Liang B, Guo X, Guo Z, Song R, Hou G, Yuan J, Yuan J, et al: Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells. Mol Med Rep 17: 2817-2826, 2018.
APA
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X. ... Zhang, Y. (2018). Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells. Molecular Medicine Reports, 17, 2817-2826. https://doi.org/10.3892/mmr.2017.8228
MLA
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X., Guo, Z., Song, R., Hou, G., Yuan, J., Liu, Y., Zhang, Y."Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells". Molecular Medicine Reports 17.2 (2018): 2817-2826.
Chicago
Liu, Z., Chai, T., Zhang, Y., Chu, A., Liang, B., Guo, X., Guo, Z., Song, R., Hou, G., Yuan, J., Liu, Y., Zhang, Y."Identification of abnormal nuclear and mitochondrial genes in esophageal cancer cells". Molecular Medicine Reports 17, no. 2 (2018): 2817-2826. https://doi.org/10.3892/mmr.2017.8228
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