Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April-2018 Volume 17 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2018 Volume 17 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis

  • Authors:
    • Jinyue He
    • Chencheng Feng
    • Jing Sun
    • Kang Lu
    • Tongwei Chu
    • Yue Zhou
    • Yong Pan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China
  • Pages: 6130-6137
    |
    Published online on: February 12, 2018
       https://doi.org/10.3892/mmr.2018.8588
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Lumbar disc disease (LDD) is common in aged populations, and it is primarily caused by intervertebral disc degeneration (IDD). Cartilage intermediate layer protein (CILP), which is specifically expressed in intervertebral discs (IVDs), is suspected to be associated with IDD. However, it remains unclear whether CILP contributes to IDD in humans. Furthermore, the regulation of CILP in human IVDs is poorly understood, especially by mechanical stimuli, which are regarded as primary factors promoting IDD. To address these issues, the present study collected nucleus pulposus (NP) cells from patients undergoing lumbar spinal surgery for degenerative disc disease (DDD). Subsequently, CILP expression was measured in human NP cells in response to mechanical stimuli, including cyclic compressive stress and cyclic tensile strain (CTS), by reverse transcription‑quantitative polymerase chain reaction and western blotting. Aggrecan and collagen II, which are the main components of the extracellular matrix (ECM) and traditional degenerative markers for IDD, were detected following the treatment with CILP small interfering (si)RNA or recombinant human CILP (rhCILP) at various concentrations to determine whether CILP contributes to IDD by negatively regulating expression of the ECM. The results revealed that CILP expression in loaded NP cells was significantly increased compared with that in non‑loaded cells under compressive loading, and that it was markedly decreased in cells under tensile loading, in contrast with the expression of aggrecan and collagen II in response to the same stimuli. Furthermore, CILP siRNA effectively inhibited CILP expression and significantly increased the expression of aggrecan and collagen II. In addition, treatment of NP cells with a high concentration of rhCILP resulted in significantly decreased expression of aggrecan and collagen II. In conclusion, these results demonstrated for the first time, to the best of our knowledge, that in human NP cells, CILP is regulated by mechanical stress and that its expression affects ECM synthesis. Therefore, CILP represents a promising therapeutic target for preventing loss of the matrix during IDD as a novel treatment strategy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Colombini A, Lombardi G, Corsi MM and Banfi G: Pathophysiology of the human intervertebral disc. Int J Biochem Cell Biol. 40:837–842. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Daly C, Ghosh P, Jenkin G, Oehme D and Goldschlager T: A review of animal models of intervertebral disc degeneration: Pathophysiology, regeneration, and translation to the clinic. Biomed Res Int. 2016:1–14. 2016. View Article : Google Scholar

3 

Seki S, Kawaguchi Y, Chiba K, Mikami Y, Kizawa H, Oya T, Mio F, Mori M, Miyamoto Y, Masuda I, et al: A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat Genet. 37:607–612. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Yee A, Lam MP, Tam V, Chan WC, Chu IK, Cheah KS, Cheung KM and Chan D: Fibrotic-like changes in degenerate human intervertebral discs revealed by quantitative proteomic analysis. Osteoarthritis Cartilage. 24:503–513. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Wang Z, Kim JH, Higashino K, Kim SS, Wang S, Seki S, Hutton WC and Yoon ST: Cartilage intermediate layer protein (CILP) regulation in intervertebral discs. The effect of age, degeneration, and bone morphogenetic protein-2. Spine (Phila Pa 1976). 37:E203–E208. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Min SK, Nakazato K, Yamamoto Y, Gushiken K, Fujimoto H, Fujishiro H, Kobayakawa Y and Hiranuma K: Cartilage intermediate layer protein gene is associated with lumbar disc degeneration in male, but not female, collegiate athletes. Am J Sports Med. 38:2552–2557. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Min SK, Nakazato K, Ishigami H and Hiranuma K: Cartilage intermediate layer protein and asporin polymorphisms are independent risk factors of lumbar disc degeneration in male collegiate athletes. Cartilage. 5:37–42. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Wang W, Hao J, Zheng S, Xiao X, Wen Y, He A, Guo X and Zhang F: Association between cartilage intermediate layer protein and degeneration of intervertebral disc: A Meta-analysis. Spine (Phila Pa 1976). 41:E1244–E1248. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Mayer JE, Iatridis JC, Chan D, Qureshi SA, Gottesman O and Hecht AC: Genetic polymorphisms associated with intervertebral disc degeneration. Spine J. 13:299–317. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Johnson K, Farley D, Hu SI and Terkeltaub R: One of two chondrocyte-expressed isoforms of cartilage intermediate-layer protein functions as an insulin-like growth factor 1 antagonist. Arthritis Rheum. 48:1302–1314. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Yao Z, Nakamura H, Masuko-Hongo K, Suzuki-Kurokawa M, Nishioka K and Kato T: Characterisation of cartilage intermediate layer protein (CILP)-induced arthropathy in mice. Ann Rheum Dis. 63:252–258. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Seki S, Tsumaki N, Motomura H, Nogami M, Kawaguchi Y, Hori T, Suzuki K, Yahara Y, Higashimoto M, Oya T, et al: Cartilage intermediate layer protein promotes lumbar disc degeneration. Biophys Res Commun. 446:876–881. 2014. View Article : Google Scholar

13 

Huang M, Wang HQ, Zhang Q, Yan XD, Hao M and Luo ZJ: Alterations of ADAMTSs and TIMP-3 in human nucleus pulposus cells subjected to compressive load: Implications in the pathogenesis of human intervertebral disc degeneration. J Orthop Res. 30:267–273. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Emanuel KS, Vergroesen PP, Peeters M, Holewijn RM, Kingma I and Smit TH: Poroelastic behaviour of the degenerating human intervertebral disc: A ten-day study in a loaded disc culture system. Eur Cell Mater. 29:330–341. 2015. View Article : Google Scholar : PubMed/NCBI

15 

MacLean JJ, Lee CR, Alini M and Iatridis JC: Anabolic and catabolic mRNA levels of the intervertebral disc vary with the magnitude and frequency of in vivo dynamic compression. J Orthop Res. 22:1193–1200. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Gao X, Zhu Q and Gu W: Prediction of glycosaminoglycan synthesis in intervertebral disc under mechanical loading. J Biomech. 49:2655–2661. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Mori M, Nakajima M, Mikami Y, Seki S, Takigawa M, Kubo T and Ikegawa S: Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene. Biochem Biophys Res Commun. 341:121–127. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Le Maitre CL, Frain J, Millward-Sadler J, Fotheringham AP, Freemont AJ and Hoyland JA: Altered integrin mechanotransduction in human nucleus pulposus cells derived from degenerated discs. Arthritis Rheum. 60:460–469. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Iorio JA, Jakoi AM and Singla A: Biomechanics of degenerative spinal disorders. Asian Spine J. 10:377–384. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Neidlinger-Wilke C, Würtz K, Urban JP, Börm W, Arand M, Ignatius A, Wilke HJ and Claes LE: Regulation of gene expression in intervertebral disc cells by low and high hydrostatic pressure. Eur Spine J. 15 Suppl 3:S372–S378. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Li P, Gan Y, Wang H, Zhang C, Wang L, Xu Y, Song L, Li S, Li S, Ou Y and Zhou Q: Dynamic compression effects on immature nucleus pulposus: A study using a novel intelligent and mechanically active bioreactor. Int J Med Sci. 13:225–234. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Bleuel J, Zaucke F, Brüggemann GP and Niehoff A: Effects of cyclic tensile strain on chondrocyte metabolism: A systematic review. PLoS One. 10:e01198162015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
He J, Feng C, Sun J, Lu K, Chu T, Zhou Y and Pan Y: Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Mol Med Rep 17: 6130-6137, 2018.
APA
He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., & Pan, Y. (2018). Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Molecular Medicine Reports, 17, 6130-6137. https://doi.org/10.3892/mmr.2018.8588
MLA
He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., Pan, Y."Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis". Molecular Medicine Reports 17.4 (2018): 6130-6137.
Chicago
He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., Pan, Y."Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis". Molecular Medicine Reports 17, no. 4 (2018): 6130-6137. https://doi.org/10.3892/mmr.2018.8588
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Feng C, Sun J, Lu K, Chu T, Zhou Y and Pan Y: Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Mol Med Rep 17: 6130-6137, 2018.
APA
He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., & Pan, Y. (2018). Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Molecular Medicine Reports, 17, 6130-6137. https://doi.org/10.3892/mmr.2018.8588
MLA
He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., Pan, Y."Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis". Molecular Medicine Reports 17.4 (2018): 6130-6137.
Chicago
He, J., Feng, C., Sun, J., Lu, K., Chu, T., Zhou, Y., Pan, Y."Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis". Molecular Medicine Reports 17, no. 4 (2018): 6130-6137. https://doi.org/10.3892/mmr.2018.8588
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team