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Article

Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice

  • Authors:
    • Yonglai Zhang
    • Rui Sha
    • Kaiguo Wang
    • Hao Li
    • Bo Yan
    • Naibao Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China, Department of Anesthesiology, Oncology Ward, Chinese Medicine Hospital in Shandong Province, Jinan, Shandong 250117, P.R. China
  • Pages: 6873-6880
    |
    Published online on: March 7, 2018
       https://doi.org/10.3892/mmr.2018.8700
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Abstract

Tetrahydropalmatine exerts numerous pharmacological activities, including analgesic and narcotic effects; anti-arrhythmic, blood pressure lowering and cardioprotective effects; protective effects against cerebral ischemia-reperfusion injury; inhibition of platelet aggregation; prevention of ulcerative diseases and inhibition of gastric acid secretion; antitumor effects; and beneficial effects on the withdrawal symptoms associated with drug addiction. The present study aimed to investigate the protective effects of tetrahydropalmatine against ketamine‑induced learning and memory impairment in mice. The Morris water maze test and open field test were used to analyzed learning and memory impairment in mice. ELISA kits and western blotting were used to analyze oxidative stress, inflammation factors, caspease‑3 and caspase‑9, iNOS, glial fibrillary acidic protein (GFAP), glial cell‑derived neurotrophic factor (GDNF), cytochrome c and phospholipase C (PLC)‑γ1 protein expression. The results demonstrated that tetrahydropalmatine treatment significantly decreased escape latency in the learning phase and increased the number of platform site crossings in ketamine‑induced mice. In addition, tetrahydropalmatine significantly inhibited oxidative stress, inflammation and acetylcholinesterase activity, and decreased acetylcholine levels in ketamine‑induced mice. Tetrahydropalmatine also suppressed iNOS protein expression, weakened caspase‑3 and caspase‑9 activation, inhibited nuclear factor‑κB, glial fibrillary acidic protein, cytochrome c and phospholipase C‑γ1 protein expression, and induced glial cell‑derived neurotrophic factor protein expression in ketamine‑induced mice. Taken together, these results indicated that tetrahydropalmatine may protect against ketamine‑induced learning and memory impairment in mice via antioxidative, anti‑inflammatory and anti‑apoptotic mechanisms. The present study provided an experimental basis for the clinical application of tetrahydropalmatine to reduce the severe side effects associated with ketamine therapy in future studies.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Sha R, Wang K, Li H, Yan B and Zhou N: Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice. Mol Med Rep 17: 6873-6880, 2018.
APA
Zhang, Y., Sha, R., Wang, K., Li, H., Yan, B., & Zhou, N. (2018). Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice. Molecular Medicine Reports, 17, 6873-6880. https://doi.org/10.3892/mmr.2018.8700
MLA
Zhang, Y., Sha, R., Wang, K., Li, H., Yan, B., Zhou, N."Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice". Molecular Medicine Reports 17.5 (2018): 6873-6880.
Chicago
Zhang, Y., Sha, R., Wang, K., Li, H., Yan, B., Zhou, N."Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice". Molecular Medicine Reports 17, no. 5 (2018): 6873-6880. https://doi.org/10.3892/mmr.2018.8700
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Sha R, Wang K, Li H, Yan B and Zhou N: Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice. Mol Med Rep 17: 6873-6880, 2018.
APA
Zhang, Y., Sha, R., Wang, K., Li, H., Yan, B., & Zhou, N. (2018). Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice. Molecular Medicine Reports, 17, 6873-6880. https://doi.org/10.3892/mmr.2018.8700
MLA
Zhang, Y., Sha, R., Wang, K., Li, H., Yan, B., Zhou, N."Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice". Molecular Medicine Reports 17.5 (2018): 6873-6880.
Chicago
Zhang, Y., Sha, R., Wang, K., Li, H., Yan, B., Zhou, N."Protective effects of tetrahydropalmatine against ketamine-induced learning and memory injury via antioxidative, anti-inflammatory and anti-apoptotic mechanisms in mice". Molecular Medicine Reports 17, no. 5 (2018): 6873-6880. https://doi.org/10.3892/mmr.2018.8700
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