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Article Open Access

Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing

  • Authors:
    • Zhenghua Gong
    • Jialin Tang
    • Tianxin Xiang
    • Jiayu Lin
    • Chaowen Deng
    • Yanzhong Peng
    • Jie Zheng
    • Guoxin Hu
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China, Department of Public Health, Center for Disease Control and Prevention in Jiangxi, Nanchang, Jiangxi 330029, P.R. China, Department of Infectious Diseases, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Digestive Diseases, The Eighth Affiliated Hospital of Sun Yat‑sen University, Shenzhen, Guangdong 518033, P.R. China, Department of Infectious Diseases, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China, Department of Traditional Chinese Medicine, Peking University Shenzhen Hospital, Shenzhen PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 299-307
    |
    Published online on: May 7, 2018
       https://doi.org/10.3892/mmr.2018.8986
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Abstract

Liver fibrosis occurs as a result of chronic liver lesions, which may subsequently develop into liver cirrhosis and hepatocellular carcinoma. The involvement of long noncoding RNAs (lncRNAs) in liver fibrosis is being increasingly recognized. However, the exact mechanisms and functions of the majority of lncRNAs are poorly characterized. In the present study, the hepatotoxic substance carbon tetrachloride (CCl4) was employed to induce liver fibrosis in an animal model and agenome‑wide identification of lncRNAs in fibrotic liver tissues compared with CCl4 untreated liver tissues was performed using RNA sequencing. Sprague‑Dawley rats were treated with CCl4 for 8 weeks. Histopathogical alterations were observed in liver tissues, and serum levels of alanine aminotransferase, aspartate aminotransferase, transforming growth factor‑β1 and tumor necrosis factor‑α were significantly higher, in the CCl4‑treated group compared with the CCl4 untreated group. RNA sequencing of liver tissues demonstrated that 231 lncRNAs and 1,036 mRNAs were differentially expressed between the two groups. Furthermore, bioinformatics analysis demonstrated that the differentially expressed mRNAs were predominantly enriched in ʻECM‑receptor interactionʼ, ʻPI3K‑Akt signaling pathwayʼ and ʻfocal adhesionʼ pathways, all of which are essential for liver fibrosis development. Validation of 12 significantly aberrant lncRNAs by reverse transcription‑quantitative polymerase chain reaction indicated that the expression patterns of 11 lncRNAs were consistent with the sequencing data. Furthermore, overexpression of lncRNA NR_002155.1, which was markedly downregulated in CCl4‑treated liver tissues, was demonstrated to inhibit HSC‑T6 cell proliferation in vitro. In conclusion, the present study determined the expression patterns of mRNAs and lncRNAs in fibrotic liver tissue induced by CCl4. The identified differentially expressed lncRNAs may serve as novel diagnostic biomarkers and therapeutic targets for liver fibrosis.
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1 

Duval F, Moreno-Cuevas JE, Gonzalez-Garza MT, Maldonado-Bernal C and Cruz-Vega DE: Liver fibrosis and mechanisms of the protective action of medicinal plants targeting inflammation and the immune response. Int J Inflam. 2015:9434972015. View Article : Google Scholar : PubMed/NCBI

2 

Enomoto M, Morikawa H, Tamori A and Kawada N: Noninvasive assessment of liver fibrosis in patients with chronic hepatitis B. World J Gastroenterol. 20:12031–12038. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Xuan J, Chen S, Ning B, Tolleson WH and Guo L: Development of HepG2-derived cells expressing cytochrome P450s for assessing metabolism-associated drug-induced liver toxicity. Chem Biol Interact. 255:63–73. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Huang ZG, Zhai WR, Zhang YE and Zhang XR: Study of heteroserum-induced rat liver fibrosis model and its mechanism. World J Gastroenterol. 4:206–209. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Novikova IV, Hennelly SP and Sanbonmatsu KY: Sizing up long non-coding RNAs: Do lncRNAs have secondary and tertiary structure? Bioarchitecture. 2:189–199. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Geisler S and Coller J: RNA in unexpected places: Long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol. 14:699–712. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Gibb EA, Brown CJ and Lam WL: The functional role of long non-coding RNA in human carcinomas. Mol Cancer. 10:382011. View Article : Google Scholar : PubMed/NCBI

8 

Li J, Tian H, Yang J and Gong Z: Long noncoding RNAs regulate cell growth, proliferation, and apoptosis. DNA Cell Biol. 35:459–470. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Briggs JA, Wolvetang EJ, Mattick JS, Rinn JL and Barry G: Mechanisms of long Non-coding RNAs in mammalian nervous system development, plasticity, disease, and evolution. Neuron. 88:861–877. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Schmitt AM and Chang HY: Long noncoding RNAs in cancer pathways. Cancer Cell. 29:452–463. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Roberts TC, Morris KV and Weinberg MS: Perspectives on the mechanism of transcriptional regulation by long non-coding RNAs. Epigenetics. 9:13–20. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Wang KC and Chang HY: Molecular mechanisms of long noncoding RNAs. Mol Cell. 43:904–914. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Yu F, Zheng J, Mao Y, Dong P, Li G, Lu Z, Guo C, Liu Z and Fan X: Long non-coding RNA APTR promotes the activation of hepatic stellate cells and the progression of liver fibrosis. Biochem Biophys Res Commun. 463:679–685. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Wu Y, Liu X, Zhou Q, Huang C, Meng X, Xu F and Li J: Silent information regulator 1 (SIRT1) ameliorates liver fibrosis via promoting activated stellate cell apoptosis and reversion. Toxicol Appl Pharmacol. 289:163–176. 2015. View Article : Google Scholar : PubMed/NCBI

15 

He Y, Wu YT, Huang C, Meng XM, Ma TT, Wu BM, Xu FY, Zhang L, Lv XW and Li J: Inhibitory effects of long noncoding RNA MEG3 on hepatic stellate cells activation and liver fibrogenesis. Biochim Biophys Acta. 1842:2204–2215. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Yu F, Zheng J, Mao Y, Dong P, Lu Z, Li G, Guo C, Liu Z and Fan X: Long Non-coding RNA growth Arrest-specific transcript 5 (GAS5) inhibits liver fibrogenesis through a mechanism of competing endogenous RNA. J Biol Chem. 290:28286–28298. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Kong X, Zhou W, Wan JB, Zhang Q, Ni J and Hu Y: An integrative thrombosis network: Visualization and topological analysis. Evid Based Complement Alternat Med. 2015:2653032015. View Article : Google Scholar : PubMed/NCBI

18 

National Research Council, . Guide for the Care and Use of Laboratory Animals. The National Academies Press; Washington, DC: 1996, https://www.nap.edu/catalog/5140/guide-for-the-care-and-use-of-laboratory-animals

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Trapnell C, Pachter L and Salzberg SL: TopHat: Discovering splice junctions with RNA-Seq. Bioinformatics. 25:1105–1111. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Feng J, Meyer CA, Wang Q, Liu JS, Liu Shirley X and Zhang Y: GFOLD: A generalized fold-change for ranking differentially expressed genes from RNA-seq data. Bioinformatics. 28:2782–2788. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL and Pachter L: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc. 7:562–578. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Dong S, Chen QL, Song YN, Sun Y, Wei B, Li XY, Hu YY, Liu P and Su SB: Mechanisms of CCl4-induced liver fibrosis with combined transcriptomic and proteomic analysis. J Toxicol Sci. 41:561–572. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Marques TG, Chaib E, da Fonseca JH, Lourenço AC, Silva FD, Ribeiro MA Jr, Galvão FH and D'Albuquerque LA: Review of experimental models for inducing hepatic cirrhosis by bile duct ligation and carbon tetrachloride injection. Acta Cir Bras. 27:589–594. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Sancheti S, Sancheti S and Seo SY: Ameliorative effects of 7-methylcoumarin and 7-methoxycoumarin against CCl4-induced hepatotoxicity in rats. Drug Chem Toxicol. 36:42–47. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW and Feng Y: The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci. 16:26087–26124. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Heeba GH and Mahmoud ME: Therapeutic potential of morin against liver fibrosis in rats: Modulation of oxidative stress, cytokine production and nuclear factor kappa B. Environ Toxicol Pharmacol. 37:662–671. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Duval F, Moreno-Cuevas JE, Gonzalez-Garza MT, Rodriguez-Montalvo C and Cruz-Vega DE: Protective mechanisms of medicinal plants targeting hepatic stellate cell activation and extracellular matrix deposition in liver fibrosis. Chin Med. 9:272014. View Article : Google Scholar : PubMed/NCBI

29 

Roy S, Benz F, Cardenas Vargas D, Vucur M, Gautheron J, Schneider A, Hellerbrand C, Pottier N, Alder J, Tacke F, et al: miR-30c and miR-193 are a part of the TGF-β-dependent regulatory network controlling extracellular matrix genes in liver fibrosis. J Dig Dis. 16:513–524. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Karsdal MA, Manon-Jensen T, Genovese F, Kristensen JH, Nielsen MJ, Sand JM, Hansen NU, Bay-Jensen AC, Bager CL, Krag A, et al: Novel insights into the function and dynamics of extracellular matrix in liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 308:G807–G830. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Carloni V, Romanelli RG, Pinzani M, Laffi G and Gentilini P: Focal adhesion kinase and phospholipase C gamma involvement in adhesion and migration of human hepatic stellate cells. Gastroenterology. 112:522–531. 1997. View Article : Google Scholar : PubMed/NCBI

32 

Renshaw MW, Price LS and Schwartz MA: Focal adhesion kinase mediates the integrin signaling requirement for growth factor activation of MAP kinase. J Cell Biol. 147:611–618. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Wang Y, Ma J, Chen L, Xie XL and Jiang H: Inhibition of focal adhesion kinase on hepatic Stellate-cell adhesion and migration. Am J Med Sci. 353:41–48. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Reif S, Lang A, Lindquist JN, Yata Y, Gabele E, Scanga A, Brenner DA and Rippe RA: The role of focal adhesion kinase-phosphatidylinositol 3-kinase-akt signaling in hepatic stellate cell proliferation and type I collagen expression. J Biol Chem. 278:8083–8090. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Novo E, Cannito S, Paternostro C, Bocca C, Miglietta A and Parola M: Cellular and molecular mechanisms in liver fibrogenesis. Arch Biochem Biophys. 548:20–37. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Lin X, Bai F, Nie J, Lu S, Lu C, Zhu X, Wei J, Lu Z and Huang Q: Didymin alleviates hepatic fibrosis through inhibiting ERK and PI3K/Akt pathways via regulation of raf kinase inhibitor protein. Cell Physiol Biochem. 40:1422–1432. 2016. View Article : Google Scholar : PubMed/NCBI

37 

Kim AH, Khursigara G, Sun X, Franke TF and Chao MV: Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1. Mol Cell Biol. 21:893–901. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Coffer PJ, Jin J and Woodgett JR: Protein kinase B (c-Akt): A multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 335:1–13. 1998. View Article : Google Scholar : PubMed/NCBI

39 

Ricupero DA, Poliks CF, Rishikof DC, Cuttle KA, Kuang PP and Goldstein RH: Phosphatidylinositol 3-kinase-dependent stabilization of alpha1(I) collagen mRNA in human lung fibroblasts. Am J Physiol Cell Physiol. 281:C99–C105. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gong Z, Tang J, Xiang T, Lin J, Deng C, Peng Y, Zheng J and Hu G: Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing. Mol Med Rep 18: 299-307, 2018.
APA
Gong, Z., Tang, J., Xiang, T., Lin, J., Deng, C., Peng, Y. ... Hu, G. (2018). Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing. Molecular Medicine Reports, 18, 299-307. https://doi.org/10.3892/mmr.2018.8986
MLA
Gong, Z., Tang, J., Xiang, T., Lin, J., Deng, C., Peng, Y., Zheng, J., Hu, G."Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing". Molecular Medicine Reports 18.1 (2018): 299-307.
Chicago
Gong, Z., Tang, J., Xiang, T., Lin, J., Deng, C., Peng, Y., Zheng, J., Hu, G."Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing". Molecular Medicine Reports 18, no. 1 (2018): 299-307. https://doi.org/10.3892/mmr.2018.8986
Copy and paste a formatted citation
x
Spandidos Publications style
Gong Z, Tang J, Xiang T, Lin J, Deng C, Peng Y, Zheng J and Hu G: Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing. Mol Med Rep 18: 299-307, 2018.
APA
Gong, Z., Tang, J., Xiang, T., Lin, J., Deng, C., Peng, Y. ... Hu, G. (2018). Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing. Molecular Medicine Reports, 18, 299-307. https://doi.org/10.3892/mmr.2018.8986
MLA
Gong, Z., Tang, J., Xiang, T., Lin, J., Deng, C., Peng, Y., Zheng, J., Hu, G."Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing". Molecular Medicine Reports 18.1 (2018): 299-307.
Chicago
Gong, Z., Tang, J., Xiang, T., Lin, J., Deng, C., Peng, Y., Zheng, J., Hu, G."Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing". Molecular Medicine Reports 18, no. 1 (2018): 299-307. https://doi.org/10.3892/mmr.2018.8986
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