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Article

Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth

  • Authors:
    • Hong Qian
    • Qun Huang
    • Yu‑Xiang Chen
    • Qiong Liu
    • Jing‑Xian Fang
    • Man‑Wen Ye
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Dentistry, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Dentistry, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511442, P.R. China
  • Pages: 1067-1073
    |
    Published online on: May 22, 2018
       https://doi.org/10.3892/mmr.2018.9046
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Abstract

As one type of adult stem cells (ASCs), human dental pulp stem cells (HDPSCs) have several properties, including high proliferation rate, self‑renewal capability, and multi‑lineage differentiation. However, the apoptotic mechanism underlying the development of dental pulp cells remains unclear. In the present study, a significant increase of apoptosis was observed in HDPSCs from the deciduous teeth compared with that from adult permanent teeth. In addition, the occurrence of cytochrome c expression and mitochondrial‑mediated apoptosis pathway activity in HDPSCs were confirmed by quantitative polymerase chain reaction, and western blotting. Although caspase‑8 and caspase‑9 showed higher expression in deciduous teeth than in adult permanent teeth, only the knockdown of caspase‑9 via RNA interference in HDPSC cells exhibited a significant reduction in apoptosis, and caspase‑3 expression and activity. All these results revealed that caspase‑9 and activated caspase‑3 predominantly regulates cell apoptosis in HDPSCs from deciduous teeth.
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Copy and paste a formatted citation
Spandidos Publications style
Qian H, Huang Q, Chen YX, Liu Q, Fang JX and Ye MW: Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth. Mol Med Rep 18: 1067-1073, 2018.
APA
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., & Ye, M. (2018). Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth. Molecular Medicine Reports, 18, 1067-1073. https://doi.org/10.3892/mmr.2018.9046
MLA
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., Ye, M."Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth". Molecular Medicine Reports 18.1 (2018): 1067-1073.
Chicago
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., Ye, M."Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth". Molecular Medicine Reports 18, no. 1 (2018): 1067-1073. https://doi.org/10.3892/mmr.2018.9046
Copy and paste a formatted citation
x
Spandidos Publications style
Qian H, Huang Q, Chen YX, Liu Q, Fang JX and Ye MW: Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth. Mol Med Rep 18: 1067-1073, 2018.
APA
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., & Ye, M. (2018). Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth. Molecular Medicine Reports, 18, 1067-1073. https://doi.org/10.3892/mmr.2018.9046
MLA
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., Ye, M."Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth". Molecular Medicine Reports 18.1 (2018): 1067-1073.
Chicago
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., Ye, M."Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth". Molecular Medicine Reports 18, no. 1 (2018): 1067-1073. https://doi.org/10.3892/mmr.2018.9046
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