Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth

  • Authors:
    • Hong Qian
    • Qun Huang
    • Yu‑Xiang Chen
    • Qiong Liu
    • Jing‑Xian Fang
    • Man‑Wen Ye
  • View Affiliations

  • Published online on: May 22, 2018     https://doi.org/10.3892/mmr.2018.9046
  • Pages: 1067-1073
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

As one type of adult stem cells (ASCs), human dental pulp stem cells (HDPSCs) have several properties, including high proliferation rate, self‑renewal capability, and multi‑lineage differentiation. However, the apoptotic mechanism underlying the development of dental pulp cells remains unclear. In the present study, a significant increase of apoptosis was observed in HDPSCs from the deciduous teeth compared with that from adult permanent teeth. In addition, the occurrence of cytochrome c expression and mitochondrial‑mediated apoptosis pathway activity in HDPSCs were confirmed by quantitative polymerase chain reaction, and western blotting. Although caspase‑8 and caspase‑9 showed higher expression in deciduous teeth than in adult permanent teeth, only the knockdown of caspase‑9 via RNA interference in HDPSC cells exhibited a significant reduction in apoptosis, and caspase‑3 expression and activity. All these results revealed that caspase‑9 and activated caspase‑3 predominantly regulates cell apoptosis in HDPSCs from deciduous teeth.
View Figures
View References

Related Articles

Journal Cover

July-2018
Volume 18 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Qian H, Huang Q, Chen YX, Liu Q, Fang JX and Ye MW: Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth. Mol Med Rep 18: 1067-1073, 2018
APA
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., & Ye, M. (2018). Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth. Molecular Medicine Reports, 18, 1067-1073. https://doi.org/10.3892/mmr.2018.9046
MLA
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., Ye, M."Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth". Molecular Medicine Reports 18.1 (2018): 1067-1073.
Chicago
Qian, H., Huang, Q., Chen, Y., Liu, Q., Fang, J., Ye, M."Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth". Molecular Medicine Reports 18, no. 1 (2018): 1067-1073. https://doi.org/10.3892/mmr.2018.9046