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Article

Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway

  • Authors:
    • Xiu‑Rong Zhang
    • Shi‑Yao Wang
    • Wen Sun
    • Chao Wei
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Maternal and Child Health Care of Shandong Province, Jinan, Shandong 250014, P.R. China, Department of Chinese Medicine, The Second Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510000, P.R. China, Department of Research, Beijing Splinger Medical Research Institute, Jinan, Shandong 250021, P.R. China, Department of Ophthalmology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
  • Pages: 3429-3436
    |
    Published online on: July 25, 2018
       https://doi.org/10.3892/mmr.2018.9318
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Abstract

Isoliquiritigenin (ISL) is a flavonoid extracted from licorice root, which is known to serve important antitumor roles in numerous types of cancers; however, its effect on gastric cancer remains to be elucidated. The present study aimed to explore the roles and underlying mechanisms of ISL in MKN28 gastric cancer cells. MKN28 cell proliferation was measured using the Cell Counting Kit‑8 (CCK8) assay. A Transwell assay was used to determine the effects of ISL on the migration and invasion of MKN28 cells. Apoptosis was assessed by flow cytometry, and the expression levels of apoptosis‑, autophagy‑ and signaling pathway‑related proteins were detected by western blot analysis. The results of the CCK8 assay demonstrated that ISL significantly inhibited the proliferation of MKN28 cells (P<0.05). Transwell assays demonstrated that the migration and invasion of MKN28 cells were significantly inhibited following treatment with ISL (P<0.05). Flow cytometric analysis indicated that ISL induced apoptosis of MKN28 cells. In addition, western blot analysis revealed that the ratio of microtubule‑associated proteins 1A/1B light chain 3B (LC3)II/LC3I was upregulated, as was Beclin 1 expression; however, p62 was downregulated following ISL pretreatment, thus suggesting that ISL triggered autophagy in MKN28 cells. In addition, the phosphorylation levels of protein kinase B (AKT) and mammalian target of rapamycin (mTOR) were significantly reduced following ISL treatment. These results indicated that ISL may influence apoptosis and autophagy in MKN28 cells by suppressing the phosphoinositide 3‑kinase/AKT/mTOR signaling pathway. In conclusion, the findings of the present study suggested that ISL may inhibit MKN28 cell proliferation, migration and invasion by inducing apoptosis and autophagy, implying potential as a therapeutic agent for gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang XR, Wang SY, Sun W and Wei C: Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway. Mol Med Rep 18: 3429-3436, 2018.
APA
Zhang, X., Wang, S., Sun, W., & Wei, C. (2018). Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway. Molecular Medicine Reports, 18, 3429-3436. https://doi.org/10.3892/mmr.2018.9318
MLA
Zhang, X., Wang, S., Sun, W., Wei, C."Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway". Molecular Medicine Reports 18.3 (2018): 3429-3436.
Chicago
Zhang, X., Wang, S., Sun, W., Wei, C."Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway". Molecular Medicine Reports 18, no. 3 (2018): 3429-3436. https://doi.org/10.3892/mmr.2018.9318
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang XR, Wang SY, Sun W and Wei C: Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway. Mol Med Rep 18: 3429-3436, 2018.
APA
Zhang, X., Wang, S., Sun, W., & Wei, C. (2018). Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway. Molecular Medicine Reports, 18, 3429-3436. https://doi.org/10.3892/mmr.2018.9318
MLA
Zhang, X., Wang, S., Sun, W., Wei, C."Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway". Molecular Medicine Reports 18.3 (2018): 3429-3436.
Chicago
Zhang, X., Wang, S., Sun, W., Wei, C."Isoliquiritigenin inhibits proliferation and metastasis of MKN28 gastric cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway". Molecular Medicine Reports 18, no. 3 (2018): 3429-3436. https://doi.org/10.3892/mmr.2018.9318
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