Open Access

Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease

  • Authors:
    • Chunshuang Xu
    • Guili Liu
    • Huihui Ji
    • Weihua Chen
    • Dongjun Dai
    • Zhongming Chen
    • Dongsheng Zhou
    • Lei Xu
    • Haochang Hu
    • Wei Cui
    • Lan Chang
    • Qin Zha
    • Liping Li
    • Shiwei Duan
    • Qinwen Wang
  • View Affiliations

  • Published online on: August 24, 2018     https://doi.org/10.3892/mmr.2018.9424
  • Pages: 4297-4302
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have suggested that increased opioid receptor κ1 (OPRK1) and opioid receptor δ1 (OPRD1) methylation levels are involved in Alzheimer's disease (AD). In the present study, the methylation levels of two opioid receptor genes, opioid receptor µ1 (OPRM1) and opioid related nociceptin receptor 1 (OPRL1), were analyzed for their association with AD. Gene methylation levels were measured using bisulfite pyrosequencing in DNA samples derived from blood samples of 51 AD patients and 63 controls. The results indicated that there were significantly elevated promoter methylation levels of OPRM1 and OPRL1 in AD (OPRM1: P=0.007; OPRL1: P=2.987x10‑6). Dual‑luciferase reporter gene assays demonstrated that the promoter fragments of these two genes were able to promote gene expression (OPRM1: Fold‑change=2.616, P=0.003; OPRL1: Fold change=11.395, P=0.007). In addition, receiver operating characteristic analyses further indicated that a methylation panel of four opioid receptor genes (area under the curve=0.848, sensitivity=0.723, and specificity=0.879) performed well in the prediction of AD. These results suggested that opioid receptor genes may be used as potential methylation biomarkers for the diagnosis of AD.
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November-2018
Volume 18 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu C, Liu G, Ji H, Chen W, Dai D, Chen Z, Zhou D, Xu L, Hu H, Cui W, Cui W, et al: Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease. Mol Med Rep 18: 4297-4302, 2018.
APA
Xu, C., Liu, G., Ji, H., Chen, W., Dai, D., Chen, Z. ... Wang, Q. (2018). Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease. Molecular Medicine Reports, 18, 4297-4302. https://doi.org/10.3892/mmr.2018.9424
MLA
Xu, C., Liu, G., Ji, H., Chen, W., Dai, D., Chen, Z., Zhou, D., Xu, L., Hu, H., Cui, W., Chang, L., Zha, Q., Li, L., Duan, S., Wang, Q."Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease". Molecular Medicine Reports 18.5 (2018): 4297-4302.
Chicago
Xu, C., Liu, G., Ji, H., Chen, W., Dai, D., Chen, Z., Zhou, D., Xu, L., Hu, H., Cui, W., Chang, L., Zha, Q., Li, L., Duan, S., Wang, Q."Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease". Molecular Medicine Reports 18, no. 5 (2018): 4297-4302. https://doi.org/10.3892/mmr.2018.9424