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Article

Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats

  • Authors:
    • Zhijian Wang
    • Shengbing Liu
    • Weiwei Pan
    • Yanjun Guo
    • Zhongfei Shen
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Jiaxing University, Jiaxing, Zhejiang 314000, P.R. China
  • Pages: 4587-4594
    |
    Published online on: September 3, 2018
       https://doi.org/10.3892/mmr.2018.9431
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Abstract

Major depression is a multifactorial disease. Emerging evidence has suggested that autophagy is involved in the pathological process of depressive disorders. Bafilomycin A1 (Baf A1), is an inhibitor of vacuolar H+‑ATPase that is frequently used at high concentrations to block late‑phase autophagy. However, whether Baf A1 has antidepressant effects remains to be elucidated. The current study aimed to evaluate the antidepressant effects of Baf A1 in rats with chronic unpredictable mild stress (CUMS) and its potential mechanism. The CUMS animal model was established. The sucrose preference test, open‑field test (OFT) and forced swim test (FST) were applied to evaluate the depressive behavior. Synaptic plasticity‑associated proteins synaptophysin and postsynaptic density protein 95 were measured by western blotting and immunofluorescence. Apoptosis‑ and autophagy‑associated proteins in addition to pro‑inflammatory cytokines, including interleukin‑1β and tumor necrosis factor‑α, were detected by western blotting, reverse transcription‑quantitative polymerase chain reaction or ELISA. A 4‑week treatment period with Baf A1 markedly ameliorated CUMS‑induced behavioral abnormalities, including increasing sucrose intake, improving locomotor activity in the OFT, and decreasing immobility time in the FST. In addition, treatment with Baf A1 restored the dysregulation of synaptic plasticity and inhibited neuroinflammation in rats exposed to CUMS. Furthermore, Baf A1 decreased the levels of apoptosis‑ and autophagy‑associated proteins induced by CUMS. The present study demonstrated that Bafilomycin A1 resulted in antidepressant effects in rats, which may be mediated by the reversal of apoptosis, autophagy and neuroinflammation in the hippocampus.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Liu S, Pan W, Guo Y and Shen Z: Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats. Mol Med Rep 18: 4587-4594, 2018.
APA
Wang, Z., Liu, S., Pan, W., Guo, Y., & Shen, Z. (2018). Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats. Molecular Medicine Reports, 18, 4587-4594. https://doi.org/10.3892/mmr.2018.9431
MLA
Wang, Z., Liu, S., Pan, W., Guo, Y., Shen, Z."Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats". Molecular Medicine Reports 18.5 (2018): 4587-4594.
Chicago
Wang, Z., Liu, S., Pan, W., Guo, Y., Shen, Z."Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats". Molecular Medicine Reports 18, no. 5 (2018): 4587-4594. https://doi.org/10.3892/mmr.2018.9431
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Liu S, Pan W, Guo Y and Shen Z: Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats. Mol Med Rep 18: 4587-4594, 2018.
APA
Wang, Z., Liu, S., Pan, W., Guo, Y., & Shen, Z. (2018). Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats. Molecular Medicine Reports, 18, 4587-4594. https://doi.org/10.3892/mmr.2018.9431
MLA
Wang, Z., Liu, S., Pan, W., Guo, Y., Shen, Z."Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats". Molecular Medicine Reports 18.5 (2018): 4587-4594.
Chicago
Wang, Z., Liu, S., Pan, W., Guo, Y., Shen, Z."Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats". Molecular Medicine Reports 18, no. 5 (2018): 4587-4594. https://doi.org/10.3892/mmr.2018.9431
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