Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2018 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2018 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels

  • Authors:
    • Shoudong Chai
    • Yang Yu
    • Haiming Li
    • Mingxin Gao
    • Bo Li
    • Chengxiong Gu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, P.R. China
  • Pages: 4643-4649
    |
    Published online on: September 17, 2018
       https://doi.org/10.3892/mmr.2018.9492
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Vein graft remains the most broadly applied vascular material in coronary artery bypass surgery. However, the restenosis rate of the vein bridge following angioplasty is high. The present study investigated the effect of medical adhesive on vascular intimal hyperplasia, in addition to the signal transduction mechanism. A total of 36 New Zealand white rabbits were divided into three groups at random, including the normal group, the surgery group and the medical adhesive spray group. Following surgery for transplantation of the left external jugular vein to the ipsilateral common carotid artery for 4 weeks, the thickness and area of the intima and media of the vessel were measured on formalin‑fixed, paraffin wax‑embedded pathological sections using hematoxylin‑eosin staining, and alterations in the expression of proliferating cell nuclear antigen (PCNA), platelet endothelial cell adhesion molecule 1 (PECAM‑1), vascular cell adhesion protein 1 (VCAM‑1), extracellular signal‑regulated kinase (ERK)1/2, and endothelial nitric oxide synthase (eNOS) were detected by immunohistochemical staining, reverse transcription‑quantitative polymerase chain reaction analysis and western blotting. The levels of intimal hyperplasia in the medical adhesive spray group were markedly decreased compared with the surgery group. Consistently, PCNA, PECAM‑1 and VCAM‑1 were underexpressed in the medical adhesive spray group compared with the surgery group. ERK1/2 and eNOS were underexpressed in the medical adhesive spray group compared with the surgery group. Therefore, the application of medical adhesive may inhibit intimal hyperplasia, which may be associated with the restriction of the over‑distension of the vein graft by downregulating the ERK1/2 and eNOS levels, reducing injury to the vascular intima and inhibiting the signaling pathway involved in intimal hyperplasia.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Shoab SS, Lowry D and Tiwari A: Effect of treated length in endovenous laser ablation of great saphenous vein on early outcomes. J Vasc Surg Venous Lymphat Disord. 4:416–421. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Fattoum M, Kennel S, Knez P, Schmitz-Rixen T, Khout H and Tenholt MH: Lower extremity arterial revascularization using conditioned small-diameter great saphenous vein. J Vasc Surg. 64:819–823. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Lehoux S and Jones EA: Shear stress, arterial identity and atherosclerosis. Thromb Haemost. 115:467–473. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Heo KS, Fujiwara K and Abe J: Shear stress and atherosclerosis. Mol Cells. 37:435–440. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Cunningham KS and Gotlieb AI: The role of shear stress in the pathogenesis of atherosclerosis. Lab Invest. 85:9–23. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Zeng Y and Liu J: Role of glypican-1 in endothelial NOS activation under various steady shear stress magnitudes. Exp Cell Res. 348:184–189. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Zeng Y, Waters M, Andrews A, Honarmandi P, Ebong EE, Rizzo V and Tarbell JM: Fluid shear stress induces the clustering of heparan sulfate via mobility of glypican-1 in lipid rafts. Am J Physiol Heart Circ Physiol. 305:H811–H820. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Dunn J, Simmons R, Thabet S and Jo H: The role of epigenetics in the endothelial cell shear stress response and atherosclerosis. Int J Biochem Cell Biol. 67:167–176. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Liu JX, Yan ZP, Zhang YY, Wu J, Liu XH and Zeng Y: Hemodynamic shear stress regulates the transcriptional expression of heparan sulfate proteoglycans in human umbilical vein endothelial cell. Cell Mol Biol (Noisy-le-grand). 62:1–34. 2016.

10 

Nigro P, Abe J and Berk BC: Flow shear stress and atherosclerosis: A matter of site specificity. Antioxid Redox Signal. 15:1405–1414. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Vogel V: Mechanotransduction involving multimodular proteins: Converting force into biochemical signals. Annu Rev Biophys Biomol Struct. 35:459–488. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Boehm EM, Powers KT, Kondratick CM, Spies M, Houtman JC and Washington MT: The proliferating cell nuclear antigen (PCNA)-interacting protein (PIP) motif of DNA polymerase eta mediates its interaction with the C-terminal domain of Rev1. J Biol Chem. 291:8735–8744. 2016. View Article : Google Scholar : PubMed/NCBI

13 

MacNeill SA: PCNA-binding proteins in the archaea: Novel functionality beyond the conserved core. Curr Genet. 62:527–532. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Krejcy K, Schwarzacher S, Ferber W, Plesch C, Cybulsky MI and Weidinger FF: Expression of VCAM-1 in rabbit iliac arteries is associated with vasodilator dysfunction of regenerated endothelium following balloon injury. Atherosclerosis. 122:59–67. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Liu D, Ding Z, Wu M, Xu W, Qian M, Du Q, Zhang L, Cui Y, Zheng J, Chang H, et al: The apolipoprotein A-I mimetic peptide, D-4F, alleviates ox-LDL-induced oxidative stress and promotes endothelial repair through the eNOS/HO-1 pathway. J Mol Cell Cardiol. 105:77–88. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Zhou J, Chen L, Fan Y, Jiang J and Wan J: Atorvastatin increases endothelial progenitor cells in balloon-injured mouse carotid artery. Can J Physiol Pharmacol. 92:369–374. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Zeng Y, Yao X, Chen L, Yan Z, Liu J, Zhang Y, Feng T, Wu J and Liu X: Sphingosine-1-phosphate induced epithelial-mesenchymal transition of hepatocellular carcinoma via an MMP-7/syndecan-1/TGF-β autocrine loop. Oncotarget. 7:63324–63337. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Kawasoe Y, Tsurimoto T, Nakagawa T, Masukata H and Takahashi TS: MutSalpha maintains the mismatch repair capability by inhibiting PCNA unloading. Elife. 5:e151552016. View Article : Google Scholar : PubMed/NCBI

19 

Juríková M, Danihel L', Polák Š and Varga I: Ki67, PCNA, and MCM proteins: Markers of proliferation in the diagnosis of breast cancer. Acta Histochem. 118:544–552. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Huang F, Abmayr SM and Workman JL: Limiting PCNA-unloading at the G1/S transition. Cell Cycle. 153001–3002. 2016.

21 

Boehm EM, Spies M and Washington MT: PCNA tool belts and polymerase bridges form during translesion synthesis. Nucleic Acids Res. 44:8250–8260. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Álvarez V, Viñas L, Gallego-Sánchez A, Andrés S, Sacristán MP and Bueno A: Orderly progression through S-phase requires dynamic ubiquitylation and deubiquitylation of PCNA. Sci Rep. 6:255132016. View Article : Google Scholar : PubMed/NCBI

23 

Yokoyama R, Hirakawa T, Hayashi S, Sakamoto T and Matsunaga S: Dynamics of plant DNA replication based on PCNA visualization. Sci Rep. 6:296572016. View Article : Google Scholar : PubMed/NCBI

24 

Akimoto S, Mitsumata M, Sasaguri T and Yoshida Y: Laminar shear stress inhibits vascular endothelial cell proliferation by inducing cyclin-dependent kinase inhibitor p21(Sdi1/Cip1/Waf1). Circ Res. 86:185–190. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Zhang Y, Liao B, Li M, Cheng M, Fu Y, Liu Q, Chen Q, Liu H, Fang Y, Zhang G and Yu F: Shear stress regulates endothelial cell function through SRB1-eNOS signaling pathway. Cardiovasc Ther. 34:308–313. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Feng YM, Chen XH and Zhang X: Roles of PECAM-1 in cell function and disease progression. Eur Rev Med Pharmacol Sci. 20:4082–4088. 2016.PubMed/NCBI

27 

Paddock C, Zhou D, Lertkiatmongkol P, Newman PJ and Zhu J: Structural basis for PECAM-1 homophilic binding. Blood. 127:1052–1061. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Gu P, Theiss A, Han J and Feagins LA: Increased cell adhesion molecules, PECAM-1, ICAM-3, or VCAM-1, predict increased risk for flare in patients with quiescent inflammatory bowel disease. J Clin Gastroenterol. 51:522–527. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Jiang L, Lin L, Li R, Yuan C, Xu M, Huang JH and Huang M: Dimer conformation of soluble PECAM-1, an endothelial marker. Int J Biochem Cell Biol. 77:102–108. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Chistiakov DA, Orekhov AN and Bobryshev YV: Endothelial PECAM-1 and its function in vascular physiology and atherogenic pathology. Exp Mol Pathol. 100:409–415. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Wang Z, Lei L, Cai XJ, Chen LY, Yuan M, Yang G, Huang P and Wang X: A preliminary study of pamidronic acid downregulation of angiogenic factors IGF-1/PECAM-1 expression in circulating level in bone metastatic breast cancer patients. Onco Targets Ther. 9:3147–3152. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Lertkiatmongkol P, Paddock C, Newman DK, Zhu J, Thomas MJ and Newman PJ: The role of sialylated glycans in human PECAM-1-mediated trans-homophilic interactions and endothelial cell barrier function. J Biol Chem. 291:26216–26225. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chai S, Yu Y, Li H, Gao M, Li B and Gu C: Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels. Mol Med Rep 18: 4643-4649, 2018.
APA
Chai, S., Yu, Y., Li, H., Gao, M., Li , B., & Gu, C. (2018). Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels. Molecular Medicine Reports, 18, 4643-4649. https://doi.org/10.3892/mmr.2018.9492
MLA
Chai, S., Yu, Y., Li, H., Gao, M., Li , B., Gu, C."Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels". Molecular Medicine Reports 18.5 (2018): 4643-4649.
Chicago
Chai, S., Yu, Y., Li, H., Gao, M., Li , B., Gu, C."Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels". Molecular Medicine Reports 18, no. 5 (2018): 4643-4649. https://doi.org/10.3892/mmr.2018.9492
Copy and paste a formatted citation
x
Spandidos Publications style
Chai S, Yu Y, Li H, Gao M, Li B and Gu C: Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels. Mol Med Rep 18: 4643-4649, 2018.
APA
Chai, S., Yu, Y., Li, H., Gao, M., Li , B., & Gu, C. (2018). Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels. Molecular Medicine Reports, 18, 4643-4649. https://doi.org/10.3892/mmr.2018.9492
MLA
Chai, S., Yu, Y., Li, H., Gao, M., Li , B., Gu, C."Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels". Molecular Medicine Reports 18.5 (2018): 4643-4649.
Chicago
Chai, S., Yu, Y., Li, H., Gao, M., Li , B., Gu, C."Application of medical adhesive inhibits intimal hyperplasia involving the downregulation of ERK1/2 and eNOS levels". Molecular Medicine Reports 18, no. 5 (2018): 4643-4649. https://doi.org/10.3892/mmr.2018.9492
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team