miR‑127 aggravates myocardial failure by promoting the TGF‑β1/Smad3 signaling Retraction in /10.3892/mmr.2019.10442

Xu,Hainian; Li,Fengmei

doi:10.3892/mmr.2018.9514

miR‑127 aggravates myocardial failure by promoting the TGF‑β1/Smad3 signaling

Retraction in: /10.3892/mmr.2019.10442

Authors:
- Hainian Xu
- Fengmei Li
View Affiliations

Affiliations: Department of Cardiovascular Internal Medicine, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Internal Medicine, Weifang Zuoshan Central Hospital, Weifang, Shandong 261041, P.R. China
Published online on: September 27, 2018 https://doi.org/10.3892/mmr.2018.9514
Pages: 4839-4846
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Myocardial failure has a negative impact on the quality of human life. MicroRNA (miR) expression abnormalities lead to the development of many pathological conditions, including myocardial failure, and therefore the present study primarily focused on the investigation of the functions of miR‑127 in the development of myocardial failure. The miR‑127 expression levels in serum samples from patients with myocardial failure were examined. Oil red O staining was used to analyze the characteristics of the myocardium of the patients. Immunohistochemistry was used to detect fatty acid synthase (FASN), stearoyl‑CoA desaturase‑1 (SCD1) and mitochondrial brown fat uncoupling protein 1 (UCP1) protein expression in the myocardium of the patients. Furthermore, C57BL/6J (B6) mice were induced with 15 mg/kg of doxorubicin. Echocardiography was used to detect the histopathological alterations of the myocardial cells by comparison of the myocardial tissues from the myocardial failure animal model and normal C57BL/6 mice. Reverse transcription‑quantitative polymerase chain reaction was used to detect the expression levels of miR‑127 following different induction periods and immunohistochemistry was used to detect the expression of transforming growth factor‑β1 (TGF‑β1) and mothers against decapentaplegic homolog 3 (Smad3). Immunofluorescence was used to detect the expression alterations TGF‑β1/Smad3 when miR‑127 overexpression or inhibition was established. The results of the present study indicated that myocardial failure resulted in an upregulated expression of miR‑127 and severe fat accumulation. FASN, SCD1 and UCP1 were highly expressed in the myocardial failure group compared with the control. Abdominal artery contraction and the ejection fraction were significantly reduced in the DOX‑induced B6 mice. The cardiomyocytes became hypertrophic, and left ventricular systolic pressure and left ventricular maximum ejection pressure were altered following DOX induction in B6 mice. The results confirmed that miR‑127 regulates the expression of TGF‑β1/Smad3. The potential pathological mechanism of the effect of miR‑127 may be based on the upregulation of the TGF‑β1/Smad3 signaling pathway.

View Figures

View References

1	Wan G, Ji L, Xia W, Cheng L and Zhang Y: Screening genes associated with elevated neutrophiltolymphocyte ratio in chronic heart failure. Mol Med Rep. 18:1415–1422. 2018.PubMed/NCBI
2	Kannel WB: Incidence and epidemiology of heart failure. Heart Fail Rev. 5:167–173. 2000. View Article : Google Scholar : PubMed/NCBI
3	Neubauer S: The failing heart-an engine out of fuel. N Engl J Med. 356:1140–1151. 2007. View Article : Google Scholar : PubMed/NCBI
4	Gulsin GS, Shetye A, Khoo J, Swarbrick DJ, Levelt E, Lai FY, Squire IB, Arnold JR and McCann GP: Does stress perfusion imaging improve the diagnostic accuracy of late gadolinium enhanced cardiac magnetic resonance for establishing the etiology of heart failure? BMC Cardiovasc Disord. 17:982017. View Article : Google Scholar : PubMed/NCBI
5	Ingwall JS and Weiss RG: Is the failing heart energy starved? On using chemical energy to support cardiac function. Circ Res. 95:135–145. 2004. View Article : Google Scholar : PubMed/NCBI
6	Ashrafian H, Frenneaux MP and Opie LH: Metabolic mechanisms in heart failure. Circulation. 116:434–448. 2007. View Article : Google Scholar : PubMed/NCBI
7	Murakami A, Nagao K, Juni N, Hara Y and Umeda M: An N-terminal di-proline motif is essential for fatty acid-dependent degradation of Δ9-desaturase in Drosophila. J Biol Chem. 292:19976–19986. 2017. View Article : Google Scholar : PubMed/NCBI
8	Dobrzyn A and Ntambi JM: The role of stearoyl-CoA desaturase in body weight regulation. Trends Cardiovasc Med. 14:77–81. 2004. View Article : Google Scholar : PubMed/NCBI
9	Liu G, Lynch JK, Freeman J, Liu B, Xin Z, Zhao H, Serby MD, Kym PR, Suhar TS, Smith HT, et al: Discovery of potent, selective, orally bioavailable stearoyl-CoA desaturase 1 inhibitors. J Med Chem. 50:3086–3100. 2007. View Article : Google Scholar : PubMed/NCBI
10	Hess D, Chisholm JW and Igal RA: Inhibition of stearoylCoA desaturase activity blocks cell cycle progression and induces programmed cell death in lung cancer cells. PLoS One. 5:e113942010. View Article : Google Scholar : PubMed/NCBI
11	Theocharisa S, Margeli A and Kouraklis G: Peroxisome proliferator activated receptor-gamma ligands as potent antineoplastic agents. Curr Med Chem Anticancer Agents. 3:239–251. 2003. View Article : Google Scholar : PubMed/NCBI
12	Wei JJ, Wu X, Peng Y, Shi G, Basturk O, Yang X, Daniels G, Osman I, Ouyang J, Hernando E, et al: Regulation of HMGA1 expression by microRNA-296 affects prostate cancer growth and invasion. Clin Cancer Res. 17:1297–1305. 2011. View Article : Google Scholar : PubMed/NCBI
13	Zhang Z, Wan F, Zhuang Q, Zhang Y and Xu Z: Suppression of miR-127 protects PC-12 cells from LPS-induced inflammatory injury by downregulation of PDCD4. Biomed Pharmacother. 96:1154–1162. 2017. View Article : Google Scholar : PubMed/NCBI
14	Ciebiera M, Wlodarczyk M, Wrzosek M, Męczekalski B, Nowicka G, Łukaszuk K, Ciebiera M, Słabuszewska-Jóźwiak A and Jakiel G: Role of transforming growth factor β in uterine fibroid biology. Int J Mol Sci. 18:pii: E2435. 2017. View Article : Google Scholar
15	Yoshida K, Murata M, Yamaguchi T and Matsuzaki K: TGF-β/Smad signaling during hepatic fibro-carcinogenesis (review). Int J Oncol. 45:1363–1371. 2014. View Article : Google Scholar : PubMed/NCBI
16	Kamato D, Burch ML, Piva TJ, Rezaei HB, Rostam MA, Xu S, Zheng W, Little PJ and Osman N: Transforming growth factor-β signalling: Role and consequences of Smad linker region phosphorylation. Cell Signal. 25:2017–2024. 2013. View Article : Google Scholar : PubMed/NCBI
17	Wei Q, Liu Q, Ren C, Liu J, Cai W, Zhu M, Jin H, He M and Yu J: Effects of bradykinin on TGFbeta1induced epithelialmesenchymal transition in ARPE19 cells. Mol Med Rep. 17:5878–5886. 2018.PubMed/NCBI
18	Liu ZY, Qiu HO, Yuan XJ, Ni YY, Sun JJ, Jing W and Fan YZ: Suppression of lymphangiogenesis in human lymphatic endothelial cells by simultaneously blocking VEGF-C and VEGF-D/VEGFR-3 with norcantharidin. Int J Oncol. 41:1762–1772. 2012. View Article : Google Scholar : PubMed/NCBI
19	Lequerica-Fernandez P, Astudillo A and de Vicente JC: Expression of vascular endothelial growth factor in salivary gland carcinomas correlates with lymph node metastasis. Anticancer Res. 27:3661–3666. 2007.PubMed/NCBI
20	Panagiotaki KN, Sideratou Z, Vlahopoulos SA, Paravatou-Petsotas M, Zachariadis M, Khoury N, Zoumpourlis V and Tsiourvas D: A Triphenylphosphonium-Functionalized Mitochondriotropic nanocarrier for efficient Co-Delivery of doxorubicin and chloroquine and enhanced antineoplastic activity. Pharmaceuticals (Basel). 10:pii: E91. 2017. View Article : Google Scholar : PubMed/NCBI
21	Chen X, Guo Z, Wang P and Xu M: Erythropoietin modulates imbalance of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in doxorubicin-induced cardiotoxicity. Heart Lung Circ. 23:772–777. 2014. View Article : Google Scholar : PubMed/NCBI
22	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
23	Adult Acute Myeloid Leukemia Treatment (PDQ(R)): Patient VersionPDQ Cancer Information Summaries. Bethesda (MD): 2002
24	Magadum A, Ding Y, He L, Kim T, Vasudevarao MD, Long Q, Yang K, Wickramasinghe N, Renikunta HV, Dubois N, et al: Live cell screening platform identifies PPARδ as a regulator of cardiomyocyte proliferation and cardiac repair. Cell Res. 27:1002–1019. 2017. View Article : Google Scholar : PubMed/NCBI
25	Hu J, Cheng P, Huang GY, Cai GW, Lian FZ, Wang XY and Gao S: Effects of Xin-Ji-Er-Kang on heart failure induced by myocardial infarction: Role of inflammation, oxidative stress and endothelial dysfunction. Phytomedicine. 42:245–257. 2018. View Article : Google Scholar : PubMed/NCBI
26	Andrés-Manzano MJ, Andrés V and Dorado B: Oil Red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root. Methods Mol Biol. 1339:85–99. 2015. View Article : Google Scholar : PubMed/NCBI
27	Tedesco FS, Dellavalle A, Diaz-Manera J, Messina G and Cossu G: Repairing skeletal muscle: Regenerative potential of skeletal muscle stem cells. J Clin Invest. 120:11–19. 2010. View Article : Google Scholar : PubMed/NCBI
28	Charge SB and Rudnicki MA: Cellular and molecular regulation of muscle regeneration. Physiol Rev. 84:209–238. 2004. View Article : Google Scholar : PubMed/NCBI
29	Huan L, Bao C, Chen D, Li Y, Lian J, Ding J, Huang S, Liang L and He X: MicroRNA-127-5p targets the biliverdin reductase B/nuclear factor-kB pathway to suppress cell growth in hepatocellular carcinoma cells. Cancer Sci. 107:258–266. 2016. View Article : Google Scholar : PubMed/NCBI
30	Ying H, Kang Y, Zhang H, Zhao D, Xia J, Lu Z, Wang H, Xu F and Shi L: MiR-127 modulates macrophage polarization and promotes lung inflammation and injury by activating the JNK pathway. J Immunol. 194:1239–1251. 2015. View Article : Google Scholar : PubMed/NCBI
31	D'Arpino MC, Fuchs AG, Sánchez SS and Honoré SM: Extracellular matrix remodeling and TGF-β1/Smad signaling in diabetic colon mucosa. Cell Biol Int. 42:443–456. 2018. View Article : Google Scholar : PubMed/NCBI
32	Gressner AM and Weiskirchen R: Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGF-beta as major players and therapeutic targets. J Cell Mol Med. 10:76–99. 2006. View Article : Google Scholar : PubMed/NCBI
33	Liu X, Hu H and Yin JQ: Therapeutic strategies against TGF-beta signaling pathway in hepatic fibrosis. Liver Int. 26:8–22. 2006. View Article : Google Scholar : PubMed/NCBI