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Article Open Access

Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury

  • Authors:
    • Shuping Li
    • Jiaxuan He
  • View Affiliations / Copyright

    Affiliations: Anesthesiology Department, Xinjiang Uygur Autonomous Region Hospital of TCM, Urumchi, Xinjiang 830000, P.R. China, Anesthesiology Department, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5353-5360
    |
    Published online on: October 24, 2018
       https://doi.org/10.3892/mmr.2018.9582
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Abstract

Pilose antler polypeptide (PAP) is an active substance isolated from the traditional Chinese medicine pilose antler, which possesses multiple biological activities. In the present study, the role and mechanism of PAP in sevoflurane (SEV)‑induced neurocyte injury was explored. Cell viability was determined by Cell Counting kit‑8 assay. Cell proliferation and apoptosis were analyzed by flow cytometry. Western blotting and reverse transcription‑quantitative polymerase chain reaction analysis were used to evaluate the protein and mRNA expression levels, respectively. The results revealed that PAP enhanced the cell viability of SEV‑treated nerve cells. In addition, through modulation of apoptosis‑associated protein expression, PAP suppressed SEV‑induced nerve cell apoptosis. Furthermore, PAP activated the p38 mitogen‑activated protein kinase (p38)/c‑Jun N‑terminal kinase (JNK) pathway in the neurocyte injury model, whereas inhibition of the p38/JNK pathway reversed the beneficial effects produced by PAP. In conclusion, PAP protected against SEV‑mediated neurocyte injury via upregulation of the p38/JNK pathway. The present findings suggested that PAP may be an effective agent for neurocyte injury.
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Copy and paste a formatted citation
Spandidos Publications style
Li S and He J: Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury. Mol Med Rep 18: 5353-5360, 2018.
APA
Li, S., & He, J. (2018). Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury. Molecular Medicine Reports, 18, 5353-5360. https://doi.org/10.3892/mmr.2018.9582
MLA
Li, S., He, J."Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury". Molecular Medicine Reports 18.6 (2018): 5353-5360.
Chicago
Li, S., He, J."Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury". Molecular Medicine Reports 18, no. 6 (2018): 5353-5360. https://doi.org/10.3892/mmr.2018.9582
Copy and paste a formatted citation
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Spandidos Publications style
Li S and He J: Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury. Mol Med Rep 18: 5353-5360, 2018.
APA
Li, S., & He, J. (2018). Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury. Molecular Medicine Reports, 18, 5353-5360. https://doi.org/10.3892/mmr.2018.9582
MLA
Li, S., He, J."Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury". Molecular Medicine Reports 18.6 (2018): 5353-5360.
Chicago
Li, S., He, J."Pilose antler polypeptide protects against sevoflurane‑mediated neurocyte injury". Molecular Medicine Reports 18, no. 6 (2018): 5353-5360. https://doi.org/10.3892/mmr.2018.9582
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