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Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis

  • Authors:
    • Shangping Zhao
    • Jinming Yang
    • Ting Liu
    • Juanxian Zeng
    • Liangliang Mi
    • Kaimin Xiang
  • View Affiliations / Copyright

    Affiliations: ICU, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Gastrointestinal Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of General Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5345-5352
    |
    Published online on: October 25, 2018
       https://doi.org/10.3892/mmr.2018.9595
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Abstract

Severe acute pancreatitis (SAP) starts as a local inflammation of pancreatic tissue that induces the development of multiple extra‑pancreatic organ dysfunction; however, the underlying mechanisms remain unclear. The present study was designed to evaluate the effect of dexamethasone (DXM) on pancreatic damage and to investigate the role of high‑mobility group box‑1 (HMGB1) and nuclear factor‑κB (NF‑κBp65) in the development of SAP in animal and cell models. For the in vivo experiment, 35 Sprague‑Dawley rats were randomly assigned to three groups: The sham‑operation control group, the SAP group and the DXM treatment group. Histological analysis revealed that, when DXM was infused into SAP rats, edema formation and structural alterations with necrosis were reduced, and the number of apoptotic cells was markedly reduced. In addition, compared with the SAP group, the expression level of HMGB1 was significantly decreased in the nucleus and the expression level of NF‑κBp65 was significantly decreased in the cytoplasm from rats treated with DXM. In vitro, DXM was able to suppress the apoptosis and cell death induced by caerulein (CAE), and DXM could suppress the expression of NF‑κBp65 and HMGB1 induced by CAE, as demonstrated by western blotting and immunofluorescence analysis. Therefore, these results provide an experimental basis for investigating the underlying therapeutic mechanisms of DXM treatment for SAP.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao S, Yang J, Liu T, Zeng J, Mi L and Xiang K: Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis. Mol Med Rep 18: 5345-5352, 2018.
APA
Zhao, S., Yang, J., Liu, T., Zeng, J., Mi, L., & Xiang, K. (2018). Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis. Molecular Medicine Reports, 18, 5345-5352. https://doi.org/10.3892/mmr.2018.9595
MLA
Zhao, S., Yang, J., Liu, T., Zeng, J., Mi, L., Xiang, K."Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis". Molecular Medicine Reports 18.6 (2018): 5345-5352.
Chicago
Zhao, S., Yang, J., Liu, T., Zeng, J., Mi, L., Xiang, K."Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis". Molecular Medicine Reports 18, no. 6 (2018): 5345-5352. https://doi.org/10.3892/mmr.2018.9595
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao S, Yang J, Liu T, Zeng J, Mi L and Xiang K: Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis. Mol Med Rep 18: 5345-5352, 2018.
APA
Zhao, S., Yang, J., Liu, T., Zeng, J., Mi, L., & Xiang, K. (2018). Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis. Molecular Medicine Reports, 18, 5345-5352. https://doi.org/10.3892/mmr.2018.9595
MLA
Zhao, S., Yang, J., Liu, T., Zeng, J., Mi, L., Xiang, K."Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis". Molecular Medicine Reports 18.6 (2018): 5345-5352.
Chicago
Zhao, S., Yang, J., Liu, T., Zeng, J., Mi, L., Xiang, K."Dexamethasone inhibits NF‑кBp65 and HMGB1 expression in the pancreas of rats with severe acute pancreatitis". Molecular Medicine Reports 18, no. 6 (2018): 5345-5352. https://doi.org/10.3892/mmr.2018.9595
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