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Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation

  • Authors:
    • Yingjian Gao
    • Bo Wang
    • Conghuan Shen
    • Weiwei Xin
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Renji Hospital, South Campus, School of Medicine, Shanghai Jiaotong University, Minhang, Shanghai 201100, P.R. China, Second Department of Orthopaedics, Baoding No. 1 Central Hospital, Baoding, Hebei 300000, P.R. China, General Surgery Department, Affiliated Huashan Hospital of Fudan University, Jingan, Shanghai 200040, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5481-5488
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    Published online on: October 30, 2018
       https://doi.org/10.3892/mmr.2018.9610
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Abstract

The concept that inflammation serves a leading role in osteoclast‑induced bone loss under pathological circumstances is now widely accepted. In the present study, it was observed that lipopolysaccharides (LPSs) demonstrated a synergic effect on receptor activator of nuclear factor κ‑B ligand (RANKL)‑induced osteoclast differentiation in Raw264.7 cells, with increasing levels of multiple pro‑inflammatory cytokines including interleukin (IL)‑6, tumor necrosis factor‑α and IL‑1β. Furthermore, microRNA (miR)‑146a was highly induced by LPS and RANKL co‑stimulation during the process of osteoclast differentiation. Overexpression of miR‑146a inhibited osteoclast transformation by targeting the key regulators of nuclear factor (NF)‑κβ signaling, TNF receptor‑associated factor 6 and interleukin‑1 receptor‑associated kinase 1. The downstream activation of NF‑κβ signaling was also inhibited by transfection with a miR‑146a mimic. Altogether, the results of the present study demonstrated that miR‑146a prevents osteoclast differentiation induced by LPS and RANKL co‑stimulation, suggesting that miR‑146a may be a promising therapeutic target for treatment of inflammation mediated bone loss.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, Wang B, Shen C and Xin W: Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation. Mol Med Rep 18: 5481-5488, 2018.
APA
Gao, Y., Wang, B., Shen, C., & Xin, W. (2018). Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation. Molecular Medicine Reports, 18, 5481-5488. https://doi.org/10.3892/mmr.2018.9610
MLA
Gao, Y., Wang, B., Shen, C., Xin, W."Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation". Molecular Medicine Reports 18.6 (2018): 5481-5488.
Chicago
Gao, Y., Wang, B., Shen, C., Xin, W."Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation". Molecular Medicine Reports 18, no. 6 (2018): 5481-5488. https://doi.org/10.3892/mmr.2018.9610
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, Wang B, Shen C and Xin W: Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation. Mol Med Rep 18: 5481-5488, 2018.
APA
Gao, Y., Wang, B., Shen, C., & Xin, W. (2018). Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation. Molecular Medicine Reports, 18, 5481-5488. https://doi.org/10.3892/mmr.2018.9610
MLA
Gao, Y., Wang, B., Shen, C., Xin, W."Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation". Molecular Medicine Reports 18.6 (2018): 5481-5488.
Chicago
Gao, Y., Wang, B., Shen, C., Xin, W."Overexpression of miR‑146a blocks the effect of LPS on RANKL‑induced osteoclast differentiation". Molecular Medicine Reports 18, no. 6 (2018): 5481-5488. https://doi.org/10.3892/mmr.2018.9610
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