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(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways

  • Authors:
    • Yadong Liu
    • Tao Yang
    • Ting Chen
    • Jun Hao
    • Yu Gai
    • Weiguo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Joint Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Joint Surgery and Sports Medicine, Tianjin Union Medical Center, Tianjin 300121, P.R. China, Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Pathology, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 116033, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 461-467
    |
    Published online on: November 23, 2018
       https://doi.org/10.3892/mmr.2018.9690
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Abstract

Lonicera japonica has been used in traditional Chinese medicine as an important medicinal plant, with the ability to inhibit osteoclast development and bone loss. However, it is not clear which active ingredient exerts these effects. (R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside (DAG) is an active constituent isolated from Lonicera japonica. In the present study, the ST2 bone marrow stromal cell line was treated by DAG at different concentrations and the osteoblastic differentiation was explored by ELISA assay, Von Kossa staining, Alizarin Red S staining, reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The results revealed that DAG promoted osteoblastic differentiation, as evidenced by increasing mineralization and alkaline phosphatase (ALP) activity, as well as the expression of genes encoding bone differentiation markers, including Alp, osteopontin (Opn) and osteocalcin (Ocn). In addition, DAG upregulated the gene expression of bone morphogenetic protein (Bmp)‑2, Bmp4, Wnt family member (Wnt)‑1, Wnt3 and runt‑related transcription factor 2 (Runx2), as well as the protein expression of phosphorylated‑mothers against decapentaplegic homolog (Smad) 1, Smad5 Smad8, β‑catenin and Runx2 in ST2 cells. The osteogenic effects induced by DAG were attenuated by the BMP antagonist Noggin and the WNT signaling pathway inhibitor Dickkopf related protein‑1. The data indicated that DAG promoted the osteoblastic differentiation of ST2 cells, at least partially through regulating the BMP/WNT signaling pathways. This provides scientific rationale for the development of DAG as a treatment for bone loss‑associated diseases, such as osteoporosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Yang T, Chen T, Hao J, Gai Y and Zhang W: (R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways. Mol Med Rep 19: 461-467, 2019.
APA
Liu, Y., Yang, T., Chen, T., Hao, J., Gai, Y., & Zhang, W. (2019). (R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways. Molecular Medicine Reports, 19, 461-467. https://doi.org/10.3892/mmr.2018.9690
MLA
Liu, Y., Yang, T., Chen, T., Hao, J., Gai, Y., Zhang, W."(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways". Molecular Medicine Reports 19.1 (2019): 461-467.
Chicago
Liu, Y., Yang, T., Chen, T., Hao, J., Gai, Y., Zhang, W."(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways". Molecular Medicine Reports 19, no. 1 (2019): 461-467. https://doi.org/10.3892/mmr.2018.9690
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Yang T, Chen T, Hao J, Gai Y and Zhang W: (R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways. Mol Med Rep 19: 461-467, 2019.
APA
Liu, Y., Yang, T., Chen, T., Hao, J., Gai, Y., & Zhang, W. (2019). (R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways. Molecular Medicine Reports, 19, 461-467. https://doi.org/10.3892/mmr.2018.9690
MLA
Liu, Y., Yang, T., Chen, T., Hao, J., Gai, Y., Zhang, W."(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways". Molecular Medicine Reports 19.1 (2019): 461-467.
Chicago
Liu, Y., Yang, T., Chen, T., Hao, J., Gai, Y., Zhang, W."(R)‑dehydroxyabscisic alcohol β‑D‑apiofuranosyl‑(1ˮ→6ʼ)‑β‑D‑glucopyranoside enhances the osteoblastic differentiation of ST2 cells via the BMP/WNT pathways". Molecular Medicine Reports 19, no. 1 (2019): 461-467. https://doi.org/10.3892/mmr.2018.9690
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