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Article Open Access

Irisin regulates the expression of BDNF and glycometabolism in diabetic rats

  • Authors:
    • Lingning Huang
    • Sunjie Yan
    • Li Luo
    • Liyong Yang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Geratology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1074-1082
    |
    Published online on: December 12, 2018
       https://doi.org/10.3892/mmr.2018.9743
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Abstract

Irisin is a proteolytic product of the fibronectin type III domain‑containing protein 5. The aim of the present study was to verify whether irisin is involved in the pathogenesis of diabetic mild cognitive impairment and elucidate the associated mechanisms. Diabetic rats were divided into four groups: Control, Model, Irisin (overexpression of irisin) and Irisin‑short hairpin (sh)‑RNA (irisin interference). The levels of irisin, brain‑derived neurotrophic factor (BDNF), glycosylated hemoglobin A1c (GHbA1c) and advanced glycated end products (AGEs) in the serum were determined using ELISA. The expression of BDNF in the hippocampal tissue was evaluated by immunohistochemical analysis. Compared with the Control group, the levels of irisin and BDNF were markedly decreased in the Model and Irisin‑shRNA groups, whereas those of GHbA1c and AGEs were markedly increased. However, the levels of irisin and BDNF in the Irisin group were significantly higher than those in the Model group, whereas the levels of GHbA1c and AGEs in the Irisin group were significantly lower. Irisin‑shRNA significantly downregulated the expression of irisin and BDNF, and upregulated the levels of GHbA1c and AGEs, compared with those in the Model group. Rat primary hippocampal nerve cells were isolated and identified by microtubule‑associated protein‑2 labeling. The vitality of primary cells from diabetic rats, evaluated using a methyl thiazolyl tetrazolium assay, was markedly decreased and further reduced following the injection of irisin‑shRNA, however, it was markedly improved following irisin treatment. The mRNA and protein levels of BDNF in the primary cells were evaluated by fluorogenic reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively, following the exposure of cells to different concentrations of glucose: 0 (control), 5.5, 15 and 25 mmol/l for 12, 24 and 48 h. The mRNA and protein expression levels of BDNF in the primary cells following exposure to glucose were significantly lower than those observed in the control. Further exposure to glucose led to a significant decrease in the expression of BDNF. In conclusion, irisin may regulate the expression of BDNF and glycometabolism in diabetic rats.
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1 

Schnaider Beeri M, Goldbourt U, Silverman JM, Noy S, Schmeidler J, Ravona-Springer R, Sverdlick A and Davidson M: Diabetes mellitus in midlife and the risk of dementia three decades later. Neurology. 63:1902–1907. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Su J, Li H and Yang W: Diabetes associated cognitive impairment: A problem should not be ignored. Chin J Endocrinol Metab. 24:476–479. 2008.

3 

Qu MH, Fang CY, Zhang XR, Zhao CZ, Mao SM and Gao ZQ: Type 2 diabetes and mild cognitive impairment. Prog Biochem Biophys. 39:791–795. 2012. View Article : Google Scholar

4 

Munshi M, Grande L, Hayes M, Ayres D, Suhl E, Capelson R, Lin S, Milberg W and Weinger K: Cognitive dysfunction is associated with poor diabetes control in older adults. Diabetes Care. 29:1794–1799. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Prada PO, Zecchin HG, Gasparetti AL, Torsoni MA, Ueno M, Hirata AE, Corezola do Amaral ME, Höer NF, Boschero AC and Saad MJ: Western diet modulates insulin signaling, c-Jun N-terminal kinase activity and insulin receptor substrate-1ser307 phosphorylation in a tissue-specific fashion. Endocrinology. 146:1576–1587. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Irie F, Fitzpatrick AL, Lopez OL, Kuller LH, Peila R, Newman AB and Launer LJ: Enhanced risk for Alzheimer disease in persons with type 2 diabetes: The Cardiovascular Health Study Cognition Study. Arch Neural. 65:89–93. 2008. View Article : Google Scholar

7 

De Matteis R, Lucertini F, Guescini M, Polidori E, Zeppa S, Stocchi V, Cinti S and Cuppini R: Exercise as a new physiological stimulus for brown adipose tissue activity. Nutr Metab Cardiovasc Dis. 23:582–590. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Timmons JA, Baar K, Davidsen PK and Atherton PJ: Is irisin a human exercise gene? Nature. 488:E9–E11. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Erden Y, Tekin S, Sandal S, Onalan EE, Tektemur A and Kirbag S: Effects of central irisin administration on the uncoupling proteins in rat brain. Neurosci Lett. 618:6–13. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Boström EA, Choi JH, Long JZ, et al: A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 481:463–468. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Huh JY, Panagiotou G, Mougios V, Brinkoetter M, Vamvini MT, Schneider BE and Mantzoros CS: FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism. 61:1725–1738. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Castillo-Quan JI: From white to brown fat through the PGC-1α-dependent myokine irisin: Implications for diabetes and obesity. Dis Model Mech. 5:293–295. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Sharma N, Castorena CM and Cartee GD: Greater insulin sensitivity in calorie restricted rats occurs with unaltered circulating levels of several important myokines and cytokines. Nutr Metab (Lond). 9:902012. View Article : Google Scholar : PubMed/NCBI

14 

Teufel A, Malik N, Mukhopadhyay M and Westphal H: Frcp1 and Frcp2, two novel fibronectin type III repeat containing genes. Gene. 297:79–83. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Ferrer-Martínez A, Ruiz-Lozano P and Chien KR: Mouse PeP: A novel peroxisomal protein linked to myoblast differentiation and development. Dev Dyn. 224:154–167. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Takayama Y: Mild cognitive impairment (MCI) and visual symptoms. Neuro-Ophthalmol Japan. 30:259–266. 2013.

18 

Failla MD, Conley YP and Wagner AK: Brain-derived neurotrophic factor (BDNF) in traumatic brain injury-related mortality: Interrelationships between genetics and acute systemic and central nervous system BDNF profiles. Neurorehabil Neural Repair. 30:83–93. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Schneider B, Prvulovic D, Oertel-Knöchel V, Knöchel C, Reinke B, Grexa M, Weber B and Hampel H: Biomarkers for major depression and its delineation from neurodegenerative disorders. Prog Neurobiol. 95:703–717. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Zou Y, Qing L, Zeng X, Shen Y, Zhong Y, Liu J, Li Q, Chen K, Lv Y, Huang D, et al: Cognitive function and plasma BDNF levels among manganese-exposed smelters. Occup Environ Med. 71:189–194. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Laske C, Stransky E, Leyhe T, Eschweiler GW, Maetzler W, Wittorf A, Soekadar S, Richartz E, Koehler N, Bartels M, et al: BDNF serum and CSF concentrations in Alzheimer's disease, normal pressure hydrocephalus and healthy controls. J Psychiatr Res. 41:387–394. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Butt ZD, Hackett JD and Volkoff H: Irisin in goldfish (Carassius auratus): Effects of irisin injections on feeding behavior and expression of appetite regulators, uncoupling proteins and lipoprotein lipase, and fasting-induced changes in FNDC5 expression. Peptides. 90:27–36. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Esposito K, Chiodini P, Maiorino MI, Capuano A, Cozzolino D, Petrizzo M, Bellastella G and Giugliano D: A nomogram to estimate the HbA1c response to different DPP-4 inhibitors in type 2 diabetes: A systematic review and meta-analysis of 98 trials with 24 163 patients. BMJ Open. 5:e0058922015. View Article : Google Scholar : PubMed/NCBI

24 

Belviranli M, Okudan N, Kabak B, Erdoğan M and Karanfilci M: The relationship between brain-derived neurotrophic factor, irisin and cognitive skills of endurance athletes. Phys Sportsmed. 44:290–296. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Geijselaers SLC, Sep SJS, Stehouwer CDA and Biessels GJ: Glucose regulation, cognition, and brain MRI in type 2 diabetes: A systematic review. Lancet Diabetes Endocrinol. 3:75–89. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Luo J, Xue H, Chen Y, Wu F, Meng G and Xu J: Effect of Ganoderma lucidum polysaccharides on expression of AGEs and AGER in myocardium of T2DM rats. Pharmacol Clin Chin Materia Medica. 27:40–43. 2011.

27 

Yurchenco PD and Schittny JC: Molecular architecture of basement membranes. FASEB J. 4:1572–1590. 1990. View Article : Google Scholar

28 

McIsaac W and Ferguson AV: Glucose concentrations modulate brain-derived neurotrophic factor responsiveness of neurones in the paraventricular nucleus of the hypothalamus. J Neuroendocrinol. 29:2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang L, Yan S, Luo L and Yang L: Irisin regulates the expression of BDNF and glycometabolism in diabetic rats. Mol Med Rep 19: 1074-1082, 2019.
APA
Huang, L., Yan, S., Luo, L., & Yang, L. (2019). Irisin regulates the expression of BDNF and glycometabolism in diabetic rats. Molecular Medicine Reports, 19, 1074-1082. https://doi.org/10.3892/mmr.2018.9743
MLA
Huang, L., Yan, S., Luo, L., Yang, L."Irisin regulates the expression of BDNF and glycometabolism in diabetic rats". Molecular Medicine Reports 19.2 (2019): 1074-1082.
Chicago
Huang, L., Yan, S., Luo, L., Yang, L."Irisin regulates the expression of BDNF and glycometabolism in diabetic rats". Molecular Medicine Reports 19, no. 2 (2019): 1074-1082. https://doi.org/10.3892/mmr.2018.9743
Copy and paste a formatted citation
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Spandidos Publications style
Huang L, Yan S, Luo L and Yang L: Irisin regulates the expression of BDNF and glycometabolism in diabetic rats. Mol Med Rep 19: 1074-1082, 2019.
APA
Huang, L., Yan, S., Luo, L., & Yang, L. (2019). Irisin regulates the expression of BDNF and glycometabolism in diabetic rats. Molecular Medicine Reports, 19, 1074-1082. https://doi.org/10.3892/mmr.2018.9743
MLA
Huang, L., Yan, S., Luo, L., Yang, L."Irisin regulates the expression of BDNF and glycometabolism in diabetic rats". Molecular Medicine Reports 19.2 (2019): 1074-1082.
Chicago
Huang, L., Yan, S., Luo, L., Yang, L."Irisin regulates the expression of BDNF and glycometabolism in diabetic rats". Molecular Medicine Reports 19, no. 2 (2019): 1074-1082. https://doi.org/10.3892/mmr.2018.9743
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