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Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway

  • Authors:
    • Chu Zhang
    • Yuchen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China, Department of Orthopedics, Wujin Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Changzhou, Jiangsu 213161, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1203-1209
    |
    Published online on: December 14, 2018
       https://doi.org/10.3892/mmr.2018.9765
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Abstract

The present study aimed to examine the roles and mechanisms of metformin in the stemness and epithelial‑mesenchymal transition (EMT) of colorectal cancer cells. The formation of spheroid cells, and the results of reverse transcription‑quantitative polymerase chain reaction and western blot analyses showed that metformin suppressed the ability to form spheroid cells and the expression of stemness markers in HCT116 colorectal cancer cells. Additionally, metformin attenuated the EMT process, characterized by a decrease of mesenchymal marker Vimentin and an increase in the expression of an epithelial marker. Mechanistically, metformin inactivated the Wnt3a/β‑catenin signaling pathway, and reactivation of Wnt3a/β‑catenin signaling attenuated the inhibition of metformin on the stemness of HCT116 colorectal cancer cells and EMT. Finally, it was revealed that metformin re‑sensitized HCT116 sphere cells to 5‑fluorouracil resistance. These results suggest that metformin can attenuate stemness and EMT in colorectal cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang C and Wang Y: Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway. Mol Med Rep 19: 1203-1209, 2019.
APA
Zhang, C., & Wang, Y. (2019). Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway. Molecular Medicine Reports, 19, 1203-1209. https://doi.org/10.3892/mmr.2018.9765
MLA
Zhang, C., Wang, Y."Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway". Molecular Medicine Reports 19.2 (2019): 1203-1209.
Chicago
Zhang, C., Wang, Y."Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway". Molecular Medicine Reports 19, no. 2 (2019): 1203-1209. https://doi.org/10.3892/mmr.2018.9765
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C and Wang Y: Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway. Mol Med Rep 19: 1203-1209, 2019.
APA
Zhang, C., & Wang, Y. (2019). Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway. Molecular Medicine Reports, 19, 1203-1209. https://doi.org/10.3892/mmr.2018.9765
MLA
Zhang, C., Wang, Y."Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway". Molecular Medicine Reports 19.2 (2019): 1203-1209.
Chicago
Zhang, C., Wang, Y."Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway". Molecular Medicine Reports 19, no. 2 (2019): 1203-1209. https://doi.org/10.3892/mmr.2018.9765
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