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In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid

  • Authors:
    • Xinyi Li
    • Zhao Chen
    • Xiaojing Li
    • Hui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 919-926
    |
    Published online on: December 14, 2018
       https://doi.org/10.3892/mmr.2018.9767
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Abstract

An increasing number of studies have demonstrated that tumor necrosis factor‑stimulated gene‑6 (TSG‑6) has a key role in the progression of fibrosis; however, the exact effects of TSG‑6 in keloid fibroblasts (KFs) remain unknown. The aim of the current study was to investigate the role of TSG‑6 in the pathogenesis of keloids. Primary fibroblasts from 10 patients with keloid were cultured and transfected with pLVX‑Puro or pLVX‑Puro‑TSG‑6. Alterations of TSG‑6 expression were then determined by reverse transcription‑polymerase chain reaction (RT‑PCR) and regulation was observed in KFs transfected with pLVX‑Puro‑TSG‑6. Compared with the control group, transfection with pLVX‑Puro‑TSG‑6 induced growth suppression, cell apoptosis and G2/M arrest in KFs. In addition, the mitochondrial apoptosis pathway was activated in KFs transfected with pLVX‑Puro‑TSG‑6. These findings indicate that TSG‑6 is a novel regulator of keloid fibrogenesis, and thus could be used/targeted TSG‑6 as a promising treatment for keloid.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Chen Z, Li X and Wang H: In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid. Mol Med Rep 19: 919-926, 2019.
APA
Li, X., Chen, Z., Li, X., & Wang, H. (2019). In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid. Molecular Medicine Reports, 19, 919-926. https://doi.org/10.3892/mmr.2018.9767
MLA
Li, X., Chen, Z., Li, X., Wang, H."In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid". Molecular Medicine Reports 19.2 (2019): 919-926.
Chicago
Li, X., Chen, Z., Li, X., Wang, H."In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid". Molecular Medicine Reports 19, no. 2 (2019): 919-926. https://doi.org/10.3892/mmr.2018.9767
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Chen Z, Li X and Wang H: In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid. Mol Med Rep 19: 919-926, 2019.
APA
Li, X., Chen, Z., Li, X., & Wang, H. (2019). In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid. Molecular Medicine Reports, 19, 919-926. https://doi.org/10.3892/mmr.2018.9767
MLA
Li, X., Chen, Z., Li, X., Wang, H."In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid". Molecular Medicine Reports 19.2 (2019): 919-926.
Chicago
Li, X., Chen, Z., Li, X., Wang, H."In vitro analysis of the role of tumor necrosis factor‑stimulated gene‑6 in keloid". Molecular Medicine Reports 19, no. 2 (2019): 919-926. https://doi.org/10.3892/mmr.2018.9767
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