Magnesium sulfate ameliorates carbon monoxide‑induced cerebral injury in male rats

Bagheri,Gholamreza; Rezaee,Ramin; Tsarouhas,Konstantinos; Docea,Anca Oana; Shahraki,Jafar; Shahriari,Malihe; Wilks,Martin F.; Jahantigh,Hosseinali; Tabrizian,Kaveh; Moghadam,Alireza Abdollahi; Bagheri,Somayeh; Spandidos,Demetrios A.; Tsatsakis,Aristidis; Hashemzaei,Mahmoud

doi:10.3892/mmr.2018.9771

Magnesium sulfate ameliorates carbon monoxide‑induced cerebral injury in male rats

Authors:
- Gholamreza Bagheri
- Ramin Rezaee
- Konstantinos Tsarouhas
- Anca Oana Docea
- Jafar Shahraki
- Malihe Shahriari
- Martin F. Wilks
- Hosseinali Jahantigh
- Kaveh Tabrizian
- Alireza Abdollahi Moghadam
- Somayeh Bagheri
- Demetrios A. Spandidos
- Aristidis Tsatsakis
- Mahmoud Hashemzaei
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Affiliations: Department of Health, Zabol University of Medical Sciences, 9861615881 Zabol, Iran, Clinical Research Unit, Faculty of Medicine, Mashhad University of Medical Sciences, 9177948564 Mashhad, Iran, Department of Cardiology, University Hospital of Larissa, 41110 Larissa, Greece, Department of Toxicology, Faculty of Pharmacy, University of Medicine and Pharmacy, 200349 Craiova, Romania, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, 9861615881 Zabol, Iran, Swiss Centre for Applied Human Toxicology, University of Basel, CH‑4055 Basel, Switzerland, Department of Pathology, Amiralmomenin Hospital, Zabol University of Medical Sciences, 9861615881 Zabol, Iran, Department of Cardiology, Emam Reza Educational Center, Mashhad University of Medical Sciences, 9137913316 Mashhad, Iran, Department of Biostatisics and Epidemiology, Faculty of Public Health, Zabol University of Medical Sciences, 9861615881 Zabol, Iran, Laboratory of Clinical Virology, Faculty of Medicine, University of Crete, 71003 Heraklion, Greece, Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, 71003 Heraklion, Greece
Published online on: December 17, 2018 https://doi.org/10.3892/mmr.2018.9771
Pages: 1032-1039
Copyright: © Bagheri et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Carbon monoxide (CO) has been shown to induce several cardiovascular abnormalities, as well as necrosis, apoptosis and oxidative stress in the brain. Magnesium sulfate (MS) has been shown to have beneficial activities against hypoxia in the brain. In the present study, the possible protective effects of MS against CO‑induced cerebral ischemia were investigated. For this purpose, 25 male Wistar rats were exposed to 3,000 ppm CO for 1 h. The animals were divided into 5 groups (n=5 in each group) as follows: The negative control group (not exposed to CO), the positive control group (CO exposed and treated with normal saline), and 3 groups of CO‑exposed rats treated with MS (75, 150 and 300 mg/kg/day) administered intraperitoneally for 5 consecutive days. On the 5th day, the animals were sacrificed and the brains were harvested for the evaluation of necrosis, apoptosis and oxidative stress. Histopathological evaluation revealed that MS reduced the number and intensity of necrotic insults. The Bax/Bcl2 ratio and malondialdehyde (MDA) levels were significantly decreased in a dose‑dependent manner in the MS‑treated rats compared to the positive control group, while a significant dose‑dependent increase in Akt expression, a pro‑survival protein, was observed. In addition, MS administration reduced pro‑apoptotic indice levels, ameliorated histological insults, favorably modulated oxidative status and increased Akt expression levels, indicating a possible neuroprotective effect in the case of CO poisoning. On the whole, the findings of this study indicate that MS may prove to be useful in protecting against CO‑induced cerebral injury.

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