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MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9

  • Authors:
    • Xuepeng Wang
    • Xi Chen
    • Lin Sun
    • Xiaoli Bi
    • Haitao He
    • Lei Chen
    • Jinfeng Pang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Affiliated Hospital of Beihua University, Jilin City, Jilin 132000, P.R. China, Department of Neurology, Affiliated Hospital of Beihua University, Jilin City, Jilin 132000, P.R. China, Department of Production, Affiliated Hospital of Beihua University, Jilin City, Jilin 132000, P.R. China, Department of CT, Affiliated Hospital of Beihua University, Jilin City, Jilin 132000, P.R. China, Department of Cycle of Ιnternal, Affiliated Hospital of Beihua University, Jilin City, Jilin 132000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 57-64
    |
    Published online on: May 13, 2019
       https://doi.org/10.3892/mmr.2019.10233
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Abstract

The present study was designed to investigate the function of matrix metalloproteinase‑9 (MMP‑9) in human glioma cells and the potential regulatory mechanisms. Reverse transcription‑quantitative polymerase chain reaction was used to analyze the expression of MMP‑9 and microRNA‑34a (miR‑34a) in the plasma of patients with glioma and healthy volunteers. MTT and Transwell assays were used to assess cell growth and migration, respectively. Annexin‑V/propidium iodide staining was used to measure cell apoptosis. In addition, MMP‑9 expression was measured using western blot analysis. In patients with glioma, MMP‑9 expression was increased, while miR‑34a expression was suppressed, compared with the normal group. Overall survival (OS) and disease‑free survival (DFS) of patients with high MMP‑9 expression were decreased compared with those with low MMP‑9 expression. OS and DFS of patients with low miR‑34a expression were decreased compared with those with high miR‑34a expression. Downregulation of miR‑34a promoted cell growth and migration, and inhibited apoptosis in U251‑MG glioma cells. However, overexpression of miR‑34a inhibited cell growth and migration, and induced apoptosis in glioma cells. Furthermore, downregulation of miR‑34a using anti‑miR‑34a induced MMP‑9 protein expression in glioma cells; whereas, overexpression of miR‑34a suppressed MMP‑9 protein expression in glioma cells. SB‑3CT, an inhibitor of MMP‑9, attenuated the effects of miR‑34a mimic on glioma cells. Together, these results indicated that miR‑34a inhibited cell growth and migration in human glioma cells by regulating MMP‑9.
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View References

1 

Wu XL, Huang H, Huang YY, Yuan JX, Zhou X and Chen YM: Reduced Pumilio-2 expression in patients with temporal lobe epilepsy and in the lithium-pilocarpine induced epilepsy rat model. Epilepsy Behav. 50:31–39. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Lin XT, Zheng XB, Fan DJ, Yao QQ, Hu JC, Lian L, Wu XJ, Lan P and He XS: MicroRNA-143 targets ATG2B to inhibit autophagy and increase inflammatory responses in crohn's disease. Inflamm Bowel Dis. 24:781–791. 2018. View Article : Google Scholar : PubMed/NCBI

3 

He M, Zhan M, Chen W, Xu S, Long M, Shen H, Shi Y, Liu Q, Mohan M and Wang J: MiR-143-5p deficiency triggers EMT and metastasis by targeting HIF-1alpha in gallbladder cancer. Cell Physiol Biochem. 42:2078–2092. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Huang FT, Peng JF, Cheng WJ, Zhuang YY, Wang LY, Li CQ, Tang J, Chen WY, Li YH and Zhang SN: MiR-143 targeting TAK1 attenuates pancreatic ductal adenocarcinoma progression via MAPK and NF-kappaB pathway in vitro. Dig Dis Sci. 62:944–957. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Cui C and Shi X: miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma. Exp Ther Med. 14:1045–1050. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Zhou W, Pal AS, Hsu AY, Gurol T, Zhu X, Wirbisky-Hershberger SE, Freeman JL, Kasinski AL and Deng Q: MicroRNA-223 suppresses the canonical NF-kappaB pathway in basal keratinocytes to dampen neutrophilic inflammation. Cell Rep. 22:1810–1823. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Chen M, Zhang H, Shi Z, Li Y, Zhang X, Gao Z, Zhou L, Ma J, Xu Q, Guan J, et al: The MST4-MOB4 complex disrupts the MST1-MOB1 complex in the Hippo-YAP pathway and plays a pro-oncogenic role in pancreatic cancer. J Biol Chem. 293:14455–14469. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Cheng J, Jing Y, Kang D, Yang L, Li J, Yu Z, Peng Z, Li X, Wei Y, Gong Q, et al: The role of Mst1 in lymphocyte homeostasis and function. Front Immunol. 9:1492018. View Article : Google Scholar : PubMed/NCBI

10 

Huang Y, Huang H, Li M, Zhang X, Liu Y and Wang Y: MicroRNA-374c-5p regulates the invasion and migration of cervical cancer by acting on the Foxc1/snail pathway. Biomed Pharmacother. 94:1038–1047. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Jia LF, Wei SB, Mitchelson K, Gao Y, Zheng YF, Meng Z, Gan YH and Yu GY: miR-34a inhibits migration and invasion of tongue squamous cell carcinoma via targeting MMP9 and MMP14. PLoS One. 9:e1084352014. View Article : Google Scholar : PubMed/NCBI

12 

Tabouret E, Bertucci F, Pierga JY, Petit T, Levy C, Ferrero JM, Campone M, Gligorov J, Lerebours F, Roché H, et al: MMP2 and MMP9 serum levels are associated with favorable outcome in patients with inflammatory breast cancer treated with bevacizumab-based neoadjuvant chemotherapy in the BEVERLY-2 study. Oncotarget. 7:18531–18540. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Martin-Alonso A, Cohen A, Quispe-Ricalde MA, Foronda P, Benito A, Berzosa P, Valladares B and Grau GE: Differentially expressed microRNAs in experimental cerebral malaria and their involvement in endocytosis, adherens junctions, FoxO and TGF-beta signalling pathways. Sci Rep. 8:112772018. View Article : Google Scholar : PubMed/NCBI

14 

Chen HX, Xu XX, Tan BZ, Zhang Z and Zhou XD: MicroRNA-29b inhibits angiogenesis by targeting VEGFA through the MAPK/ERK and PI3K/Akt signaling pathways in endometrial carcinoma. Cell Physiol Biochem. 41:933–946. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Zhao Q, Ye M, Yang W, Wang M, Li M, Gu C, Zhao L, Zhang Z, Han W, Fan W and Meng Y: Effect of Mst1 on endometriosis apoptosis and migration: Role of drp1-related mitochondrial fission and parkin-required mitophagy. Cell Physiol Biochem. 45:1172–1190. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Yang L, Song X, Zhu J, Li M, Ji Y, Wu F, Chen Y, Cui X, Hu J, Wang L, et al: Tumor suppressor microRNA-34a inhibits cell migration and invasion by targeting MMP-2/MMP-9/FNDC3B in esophageal squamous cell carcinoma. Int J Oncol. 51:378–388. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Chen X, Sun L, Bi X, He H, Chen L and Pang J: MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9. Mol Med Rep 20: 57-64, 2019.
APA
Wang, X., Chen, X., Sun, L., Bi, X., He, H., Chen, L., & Pang, J. (2019). MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9. Molecular Medicine Reports, 20, 57-64. https://doi.org/10.3892/mmr.2019.10233
MLA
Wang, X., Chen, X., Sun, L., Bi, X., He, H., Chen, L., Pang, J."MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9". Molecular Medicine Reports 20.1 (2019): 57-64.
Chicago
Wang, X., Chen, X., Sun, L., Bi, X., He, H., Chen, L., Pang, J."MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9". Molecular Medicine Reports 20, no. 1 (2019): 57-64. https://doi.org/10.3892/mmr.2019.10233
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Chen X, Sun L, Bi X, He H, Chen L and Pang J: MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9. Mol Med Rep 20: 57-64, 2019.
APA
Wang, X., Chen, X., Sun, L., Bi, X., He, H., Chen, L., & Pang, J. (2019). MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9. Molecular Medicine Reports, 20, 57-64. https://doi.org/10.3892/mmr.2019.10233
MLA
Wang, X., Chen, X., Sun, L., Bi, X., He, H., Chen, L., Pang, J."MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9". Molecular Medicine Reports 20.1 (2019): 57-64.
Chicago
Wang, X., Chen, X., Sun, L., Bi, X., He, H., Chen, L., Pang, J."MicroRNA‑34a inhibits cell growth and migration in human glioma cells via MMP‑9". Molecular Medicine Reports 20, no. 1 (2019): 57-64. https://doi.org/10.3892/mmr.2019.10233
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