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Article

Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells

  • Authors:
    • Dong Qin
    • Yanrong Jiang
    • Xuemin Jin
  • View Affiliations / Copyright

    Affiliations: Henan Eye Institute, Henan Provincial Eye Hospital, People's Hospital of Henan Province, Zhengzhou, Henan 450003, P.R. China, Department of Ophthalmology, People's Hospital of Peking University, Beijing 100044, P.R. China
  • Pages: 830-836
    |
    Published online on: May 22, 2019
       https://doi.org/10.3892/mmr.2019.10277
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Abstract

Proliferative vitreoretinopathy (PVR) is a vision‑threatening disease. It is also a common complication resulting from surgery to correct rhegmatogenous retinal detachment. Proliferation and migration of retinal pigment epithelial (RPE) cells and the secretion of extracellular matrix molecules play an important role in the formation of the preretinal membrane in PVR patients. Furthermore, upregulated expression of inflammatory cytokines within the vitreous or subretinal fluid of patients experiencing vitreoretinal disorders may aggravate the inflammatory response and be involved in the development of PVR. PVR is triggered by many inflammatory cytokines and growth factors. Macrophage migration inhibitory factor (MIF), an inflammatory cytokine, is upregulated in the vitreous in PVR patients. However, there is little known concerning the connection between MIF and the expression of inflammatory cytokines, interleukin‑6 (IL‑6) and monocyte chemotactic‑1 (MCP‑1), and the fibrogenic gene, collagen I, in human RPE cells. Cell proliferation, migration, and expression of IL‑6, MCP‑1 and collagen I were assessed using an MTT assay, a Transwell assay, real‑time PCR analysis and ELISA kits. Western‑blot analysis was used to detect phosphorylation of p38 mitogen activated protein kinase (MAPK) and extracellular signal‑regulated kinase (ERK) signaling pathways. The data revealed that MIF promoted the proliferation, migration and expression of IL‑6, MCP‑1 and collagen I, and phosphorylation of p38 and ERK signaling pathways in RPE cells in vitro. These findings suggest that MIF plays a proinflammatory and profibrotic role in the development of PVR.
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Copy and paste a formatted citation
Spandidos Publications style
Qin D, Jiang Y and Jin X: Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells. Mol Med Rep 20: 830-836, 2019.
APA
Qin, D., Jiang, Y., & Jin, X. (2019). Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells. Molecular Medicine Reports, 20, 830-836. https://doi.org/10.3892/mmr.2019.10277
MLA
Qin, D., Jiang, Y., Jin, X."Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells". Molecular Medicine Reports 20.1 (2019): 830-836.
Chicago
Qin, D., Jiang, Y., Jin, X."Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells". Molecular Medicine Reports 20, no. 1 (2019): 830-836. https://doi.org/10.3892/mmr.2019.10277
Copy and paste a formatted citation
x
Spandidos Publications style
Qin D, Jiang Y and Jin X: Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells. Mol Med Rep 20: 830-836, 2019.
APA
Qin, D., Jiang, Y., & Jin, X. (2019). Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells. Molecular Medicine Reports, 20, 830-836. https://doi.org/10.3892/mmr.2019.10277
MLA
Qin, D., Jiang, Y., Jin, X."Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells". Molecular Medicine Reports 20.1 (2019): 830-836.
Chicago
Qin, D., Jiang, Y., Jin, X."Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells". Molecular Medicine Reports 20, no. 1 (2019): 830-836. https://doi.org/10.3892/mmr.2019.10277
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