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Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells

  • Authors:
    • Jingjing Qi
    • Dan Li
    • Guoping Shi
    • Xuefang Zhang
    • Yuchen Pan
    • Huan Dou
    • Tingting Wang
    • Genhong Yao
    • Yayi Hou
  • View Affiliations / Copyright

    Affiliations: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, Jiangsu 210093, P.R. China
    Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1131-1138
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    Published online on: June 6, 2019
       https://doi.org/10.3892/mmr.2019.10352
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Abstract

Interleukin (IL)‑12 modulates the generation and function of various immune cells and plays a vital role in the pathogenesis of Sjögren's syndrome (SS). Myeloid‑derived suppressor cells (MDSCs) are involved in autoimmune diseases by regulating various immune responses. However, it has not been confirmed whether inflammatory IL‑12 participates in the progression of SS via regulating MSDCs. In the present study, the plasma levels of IL‑12 were detected by ELISA in SS‑like non‑obese diabetic (NOD) mice. The mice were treated by intraperitoneal injection of IL‑12 and anti‑IL‑12 antibody, respectively, and then the salivary flow rate was detected. The pathology of submandibular glands was evaluated in tissue sections stained with hematoxylin and eosin. The proportion of MDSCs was assessed by flow cytometry. The results showed that plasma IL‑12 was significantly increased in the SS‑like NOD mice comparing with that noted in the control mice. The exogenous IL‑12 exacerbated SS‑like symptoms of NOD mice and promoted the generation of both bone marrow (BM) and splenic MDSCs in the SS‑like NOD mice. Of note, anti‑IL‑12 alleviated SS‑like symptoms of NOD mice and inhibited the generation of BM and splenic MDSCs. Moreover, the generation of MDSCs was crippled in the IL‑12‑deficient C57BL/6 (Il‑12‑/‑ B6) mice. Our findings suggest that aggravation of SS‑like symptoms by IL‑12 in NOD mice may be attributed to its promotion of MDSC development.
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Copy and paste a formatted citation
Spandidos Publications style
Qi J, Li D, Shi G, Zhang X, Pan Y, Dou H, Wang T, Yao G and Hou Y: Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells. Mol Med Rep 20: 1131-1138, 2019.
APA
Qi, J., Li, D., Shi, G., Zhang, X., Pan, Y., Dou, H. ... Hou, Y. (2019). Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells. Molecular Medicine Reports, 20, 1131-1138. https://doi.org/10.3892/mmr.2019.10352
MLA
Qi, J., Li, D., Shi, G., Zhang, X., Pan, Y., Dou, H., Wang, T., Yao, G., Hou, Y."Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells". Molecular Medicine Reports 20.2 (2019): 1131-1138.
Chicago
Qi, J., Li, D., Shi, G., Zhang, X., Pan, Y., Dou, H., Wang, T., Yao, G., Hou, Y."Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells". Molecular Medicine Reports 20, no. 2 (2019): 1131-1138. https://doi.org/10.3892/mmr.2019.10352
Copy and paste a formatted citation
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Spandidos Publications style
Qi J, Li D, Shi G, Zhang X, Pan Y, Dou H, Wang T, Yao G and Hou Y: Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells. Mol Med Rep 20: 1131-1138, 2019.
APA
Qi, J., Li, D., Shi, G., Zhang, X., Pan, Y., Dou, H. ... Hou, Y. (2019). Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells. Molecular Medicine Reports, 20, 1131-1138. https://doi.org/10.3892/mmr.2019.10352
MLA
Qi, J., Li, D., Shi, G., Zhang, X., Pan, Y., Dou, H., Wang, T., Yao, G., Hou, Y."Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells". Molecular Medicine Reports 20.2 (2019): 1131-1138.
Chicago
Qi, J., Li, D., Shi, G., Zhang, X., Pan, Y., Dou, H., Wang, T., Yao, G., Hou, Y."Interleukin‑12 exacerbates Sjögren's syndrome through induction of myeloid‑derived suppressor cells". Molecular Medicine Reports 20, no. 2 (2019): 1131-1138. https://doi.org/10.3892/mmr.2019.10352
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