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Article

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

  • Authors:
    • Chunlin Yin
    • Xianxian Zheng
    • Heping Xiang
    • He Li
    • Ming Gao
    • Xiangling Meng
    • Kai Yang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Clinical Laboratory, Hefei Binhu Hospital, Hefei, Anhui 230601, P.R. China, Department of Emergency Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Clinical Laboratory, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China
  • Pages: 1966-1976
    |
    Published online on: June 26, 2019
       https://doi.org/10.3892/mmr.2019.10430
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Abstract

Cisplatin, one of the most commonly used drugs in combination chemotherapy, is an effective anti‑tumor agent widely used for diverse tumor types. MicroRNAs (miRNAs/miRs) are involved in the occurrence, development, diagnosis and treatment of cancer. Therefore, the aim of the current study was to explore whether cisplatin exerts anticancer effects by causing differential expression of miRNAs in human gastric cancer cells. The human gastric cancer cell line NCI‑N87 was cultured with a certain dose of cisplatin and high‑throughput sequencing combined with reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to detect cisplatin‑regulated miRNAs. miRNAs upregulated and downregulated following cisplatin exposure were analyzed. High‑throughput sequencing revealed 33 upregulated and 16 downregulated miRNAs. A total of five significantly upregulated and five significantly downregulated miRNAs were identified by RT‑qPCR. The expression levels of hsa‑miR‑1246 and hsa‑miR‑892b were consistent with the results obtained from high‑throughput sequencing. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway clustering of cisplatin‑regulated miRNAs revealed that the miRNAs regulated genes involved in several biological processes and signaling pathways. The results obtained in the current study suggested that cisplatin may exert an important anticancer effect in gastric cancer via complex biological processes and signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Yin C, Zheng X, Xiang H, Li H, Gao M, Meng X and Yang K: Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line. Mol Med Rep 20: 1966-1976, 2019.
APA
Yin, C., Zheng, X., Xiang, H., Li, H., Gao, M., Meng, X., & Yang, K. (2019). Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line. Molecular Medicine Reports, 20, 1966-1976. https://doi.org/10.3892/mmr.2019.10430
MLA
Yin, C., Zheng, X., Xiang, H., Li, H., Gao, M., Meng, X., Yang, K."Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line". Molecular Medicine Reports 20.2 (2019): 1966-1976.
Chicago
Yin, C., Zheng, X., Xiang, H., Li, H., Gao, M., Meng, X., Yang, K."Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line". Molecular Medicine Reports 20, no. 2 (2019): 1966-1976. https://doi.org/10.3892/mmr.2019.10430
Copy and paste a formatted citation
x
Spandidos Publications style
Yin C, Zheng X, Xiang H, Li H, Gao M, Meng X and Yang K: Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line. Mol Med Rep 20: 1966-1976, 2019.
APA
Yin, C., Zheng, X., Xiang, H., Li, H., Gao, M., Meng, X., & Yang, K. (2019). Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line. Molecular Medicine Reports, 20, 1966-1976. https://doi.org/10.3892/mmr.2019.10430
MLA
Yin, C., Zheng, X., Xiang, H., Li, H., Gao, M., Meng, X., Yang, K."Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line". Molecular Medicine Reports 20.2 (2019): 1966-1976.
Chicago
Yin, C., Zheng, X., Xiang, H., Li, H., Gao, M., Meng, X., Yang, K."Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line". Molecular Medicine Reports 20, no. 2 (2019): 1966-1976. https://doi.org/10.3892/mmr.2019.10430
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