Role of osteoprotegerin in the regulation of dental epithelial‑mesenchymal signaling during tooth development

Gao,Xin; Zheng,Junming; Tu,Shaoqin; Cai,Bin; Zeng,Rongsheng; Xiang,Lusai

doi:10.3892/mmr.2019.10567

Role of osteoprotegerin in the regulation of dental epithelial‑mesenchymal signaling during tooth development

Authors:
- Xin Gao
- Junming Zheng
- Shaoqin Tu
- Bin Cai
- Rongsheng Zeng
- Lusai Xiang
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Affiliations: Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Research Management Office, Foshan Stomatology Hospital, School of Stomatology and Medicine, Foshan University, Foshan, Guangdong 528000, P.R. China
Published online on: August 7, 2019 https://doi.org/10.3892/mmr.2019.10567
Pages: 3035-3042
Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dental epithelial‑mesenchymal signaling is crucial for tooth development, but the detailed mechanism is not fully understood. Using microarray analysis, it was revealed that the expression of osteoprotegerin, an important factor regulating bone remodeling, significantly increased after removal of the dental epithelium. Immunohistochemical staining revealed that osteoprotegerin expression within the dental mesenchyme was quite low during the prenatal period, but significantly increased after birth. To investigate the influence of osteoprotegerin upon tooth development, first‑molar tooth germs from embryonic day 14.5 (E14.5) Chinese Kunming mice were treated with different concentrations of osteoprotegerin. It was revealed that osteoprotegerin could inhibit the expression of odontogenic markers while promoting the expression of osteogenic markers, thereby disrupting tooth morphogenesis. These findings were further supported by in vitro and in vivo cultures. Finally, quantitative reverse transcription‑polymerase chain reaction and immunofluorescence studies revealed that, after osteoprotegerin treatment, the activity of the wingless/integrated (Wnt)/β‑catenin pathway increased, indicating that increased osteoprotegerin expression in prenatal tooth development could lead to uncontrolled upregulation of the Wnt/β‑catenin pathway.

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