MicroRNA‑125a‑5p controls the proliferation, apoptosis, migration and PTEN/MEK1/2/ERK1/2 signaling pathway in MCF‑7 breast cancer cells

Liang,Zhongzeng; Pan,Qunwen; Zhang,Zhi; Huang,Chaosheng; Yan,Zeming; Zhang,Yuanqi; Li,Jianwen

doi:10.3892/mmr.2019.10704

MicroRNA‑125a‑5p controls the proliferation, apoptosis, migration and PTEN/MEK1/2/ERK1/2 signaling pathway in MCF‑7 breast cancer cells

Authors:
- Zhongzeng Liang
- Qunwen Pan
- Zhi Zhang
- Chaosheng Huang
- Zeming Yan
- Yuanqi Zhang
- Jianwen Li
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Affiliations: Department of Vascular Thyroid Breast Surgery, Institute of Neurology, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China, Guangdong Key Laboratory of Age‑Related Cardiac and Cerebral Diseases, Institute of Neurology, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China
Published online on: September 24, 2019 https://doi.org/10.3892/mmr.2019.10704
Pages: 4507-4514
Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miR)‑125a‑5p has shown the potential for suppressing tumorigenesis and development; however, the effects of miR‑125a‑5p on breast cancer cells remains unknown. The aim of this study was to evaluate the effects and underlying mechanisms of miR‑125a‑5p in MCF‑7 breast cancer cells. MCF‑7 cells were transfected with miR‑125a‑5p mimic or miR‑125a‑5p small interfering RNA to produce miR‑125a‑5p overexpressing/knockdown cells. Cell proliferation was assessed by an MTT assay, and cell migration ability was determined by an in vitro scratch assay. Hoechst 33258 staining and flow cytometry were performed to assess the effects of miR‑125a‑5p on MCF‑7 apoptosis. Western blotting and reverse transcription‑quantitative polymerase chain reaction were used for measuring phosphatase and tensin homolog (PTEN), phosphorylated (p)‑mitogen‑activated protein kinase kinase (MEK1/2)/MEK1/2, p‑ERK1/2/ERK1/2, B‑cell lymphoma‑2 (Bcl‑2), cleaved caspase‑3, and miR‑125a‑5p expression. miR‑125a‑5p overexpression inhibited the proliferation and migration, but promoted the apoptosis of MCF‑7 cells. These effects were associated with increases in PTEN and cleaved caspase‑3 expression, and decreases in p‑MEK1/2/MEK1/2, p‑ERK1/2/ERK1/2, and Bcl‑2. Silencing of miR‑125a‑5p exhibited opposing effects on MCF‑7 cells. These observations suggested that miR‑125a‑5p participates in the regulation of multiple functions of MCF‑7 cells by promoting the expression of PTEN tumor suppressor genes, activating MEK1/2/ERK1/2 signaling, and regulating caspase‑3/Bcl‑2 signaling. Thus, it may be a suitable target for breast cancer gene therapy.

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