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Article

Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism

  • Authors:
    • Xin Zhong
    • Wei Gao
    • Runda Wu
    • Haibo Liu
    • Junbo Ge
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, P.R. China, Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China
  • Pages: 5315-5323
    |
    Published online on: October 16, 2019
       https://doi.org/10.3892/mmr.2019.10749
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Abstract

Activation of endothelial cells is the first step of atherosclerosis. The current authors have previously reported that exosomes from mature dendritic cells (mDC‑exo) participate in endothelial inflammation and atherosclerosis through membrane tumor necrosis factor‑α mediated the nuclear factor (NF)‑κB signaling pathway. However, whether mDC‑exo shuttled microRNAs (miRNAs/miRs) play a role in endothelial inflammation remains unknown. In this study, mDC‑exo were co‑cultured with human umbilical vein endothelial cells (HUVECs) and the expression of adhesion molecules, such as vascular cell adhesion molecule‑1, intercellular adhesion molecule‑1 and E‑Selectin was investigated. Then the expression of miRNAs in DC‑exo was explored and the role of miR‑146a in endothelial inflammation was investigated. mDC‑exos were first demonstrated to increase endothelial expression of adhesion molecules through a quick activation of the NF‑κB signaling pathway. Then it was demonstrated that HUVECs resistant to a second stimulation after the first stimulation by mDC‑exo. A set of miRNAs were targeted and their expression in HUVECs stimulated with mDC‑exo was measured. Finally, it was confirmed that mDC‑exo shuttles miR‑146a into HUVECs and the shuttled miR‑146a contributes to protect HUVECs from a second stimulation through inhibiting interleukin‑1 receptor‑associated kinase. These data suggest a negative feedback loop of inflammation regulation by DC‑exo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong X, Gao W, Wu R, Liu H and Ge J: Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism. Mol Med Rep 20: 5315-5323, 2019.
APA
Zhong, X., Gao, W., Wu, R., Liu, H., & Ge, J. (2019). Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism. Molecular Medicine Reports, 20, 5315-5323. https://doi.org/10.3892/mmr.2019.10749
MLA
Zhong, X., Gao, W., Wu, R., Liu, H., Ge, J."Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism". Molecular Medicine Reports 20.6 (2019): 5315-5323.
Chicago
Zhong, X., Gao, W., Wu, R., Liu, H., Ge, J."Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism". Molecular Medicine Reports 20, no. 6 (2019): 5315-5323. https://doi.org/10.3892/mmr.2019.10749
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong X, Gao W, Wu R, Liu H and Ge J: Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism. Mol Med Rep 20: 5315-5323, 2019.
APA
Zhong, X., Gao, W., Wu, R., Liu, H., & Ge, J. (2019). Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism. Molecular Medicine Reports, 20, 5315-5323. https://doi.org/10.3892/mmr.2019.10749
MLA
Zhong, X., Gao, W., Wu, R., Liu, H., Ge, J."Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism". Molecular Medicine Reports 20.6 (2019): 5315-5323.
Chicago
Zhong, X., Gao, W., Wu, R., Liu, H., Ge, J."Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism". Molecular Medicine Reports 20, no. 6 (2019): 5315-5323. https://doi.org/10.3892/mmr.2019.10749
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