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Oxyntomodulin attenuates TNF‑α induced neuropathic pain by inhibiting the activation of the NF‑κB pathway

  • Authors:
    • Yu Zhang
    • Liyong Yuan
    • Yuebo Chen
    • Chunyan Lin
    • Guangyao Ye

  • View Affiliations

  • Published online on: October 25, 2019     https://doi.org/10.3892/mmr.2019.10770
  • Pages: 5223-5228
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Neuropathic pain is rarely diagnosed. Oxyntomodulin is peripherally and centrally distributed; however, the potential mechanisms underlying the effects of oxyntomodulin in attenuating nociception remain unclear; thus, we aimed to explore them in the present study. A neuropathic pain model in male C57BL/6 mice was induced by intrathecal injection of tumor necrosis factor‑α (TNF‑α), and the duration of nociceptive behavioral responses was measured with a stop‑watch timer within 30 min. Western blotting was used to explore the protein levels of ionized calcium binding adaptor molecule‑1 (IBA1), nuclear factor‑κB (NF‑κB) phosphorylated‑p65, interleukin (IL)‑6 and IL‑1β. We performed reverse transcription‑quantitative polymerase chain reaction and ELISA were performed to determine the mRNA and protein expression levels of IL‑6 and IL‑1β, respectively. An MTT assay was conducted to detect BV2 cell viability. Oxyntomodulin was observed to attenuate TNF‑α‑induced pain hypersensitivity in mice, as well as the expression of IBA1, NF‑κB p‑p65, IL‑6 and IL‑1β in the spinal cord. Oxyntomodulin exhibited no cytotoxicity on BV2 cells, and attenuated TNF‑α‑induced IL‑6 and IL‑1β production and release in BV2 cells and culture medium, respectively. Collectively, we proposed oxyntomodulin to attenuate TNF‑α induced neuropathic pain associated with the release of glial cytokines IL‑6 and IL‑1β via inhibiting the activation of the NF‑κB pathway.
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December-2019
Volume 20 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Zhang Y, Yuan L, Chen Y, Lin C and Ye G: Oxyntomodulin attenuates TNF‑α induced neuropathic pain by inhibiting the activation of the NF‑κB pathway. Mol Med Rep 20: 5223-5228, 2019
APA
Zhang, Y., Yuan, L., Chen, Y., Lin, C., & Ye, G. (2019). Oxyntomodulin attenuates TNF‑α induced neuropathic pain by inhibiting the activation of the NF‑κB pathway. Molecular Medicine Reports, 20, 5223-5228. https://doi.org/10.3892/mmr.2019.10770
MLA
Zhang, Y., Yuan, L., Chen, Y., Lin, C., Ye, G."Oxyntomodulin attenuates TNF‑α induced neuropathic pain by inhibiting the activation of the NF‑κB pathway". Molecular Medicine Reports 20.6 (2019): 5223-5228.
Chicago
Zhang, Y., Yuan, L., Chen, Y., Lin, C., Ye, G."Oxyntomodulin attenuates TNF‑α induced neuropathic pain by inhibiting the activation of the NF‑κB pathway". Molecular Medicine Reports 20, no. 6 (2019): 5223-5228. https://doi.org/10.3892/mmr.2019.10770